Fibronectin 1 is a potential biomarker for radioresistance in head and neck squamous cell carcinoma

F. Jerhammer (Corresponding Author), R. Ceder, S. Garvin, R. Grénman, Roland Grafström, K. Roberg

Research output: Contribution to journalArticleScientificpeer-review

37 Citations (Scopus)

Abstract

Radiotherapy remains the backbone of head and neck cancer therapy but response is sometimes impeded by tumor radioresistance. Identifying predictive biomarkers of radiotherapy response is a crucial step towards personalized therapy. The aim of this study was to explore gene expression data in search of biomarkers predictive of the response to radiotherapy in head and neck squamous cell carcinoma (HNSCC).

Microarray analysis was performed on five cell lines with various intrinsic radiosensitivity, selected from a panel of 29 HNSCC cell lines. The bioinformatics approach included Gene Ontology (GO) enrichment profiling and Ingenuity Pathway Analysis (IPA). The GO-analysis detected 16 deregulated categories from which development, receptor activity, and extracellular region represented the largest groups. Fourteen hub genes (CEBPA, CEBPB, CTNNB1, FN1, MYC, MYCN, PLAU, SDC4, SERPINE1, SP1, TAF4B, THBS1, TP53 and VLDLR) were identified from the IPA network analysis. The hub genes in the highest ranked network, (FN1, SERPINE1, THBS1 and VLDLR) were further subjected to qPCR analysis in the complete panel of 29 cell lines. Of these genes, high FN1 expression associated to high intrinsic radiosensitivity (p=0.047).

In conclusion, gene ontologies and hub genes of importance for intrinsic radiosensitivity were defined. The overall results suggest that FN1 should be explored as a potential novel biomarker for radioresistance.

Original languageEnglish
Pages (from-to)1244-1251
Number of pages8
JournalCancer Biology and Therapy
Volume10
Issue number12
DOIs
Publication statusPublished - 2010
MoE publication typeA1 Journal article-refereed

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Fibronectins
Gene Ontology
Radiation Tolerance
Biomarkers
Radiotherapy
Cell Line
Genes
Microarray Analysis
Head and Neck Neoplasms
Computational Biology
Gene Expression
Carcinoma, squamous cell of head and neck
Therapeutics
Neoplasms

Cite this

Jerhammer, F., Ceder, R., Garvin, S., Grénman, R., Grafström, R., & Roberg, K. (2010). Fibronectin 1 is a potential biomarker for radioresistance in head and neck squamous cell carcinoma. Cancer Biology and Therapy, 10(12), 1244-1251. https://doi.org/10.4161/cbt.10.12.13432
Jerhammer, F. ; Ceder, R. ; Garvin, S. ; Grénman, R. ; Grafström, Roland ; Roberg, K. / Fibronectin 1 is a potential biomarker for radioresistance in head and neck squamous cell carcinoma. In: Cancer Biology and Therapy. 2010 ; Vol. 10, No. 12. pp. 1244-1251.
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Jerhammer, F, Ceder, R, Garvin, S, Grénman, R, Grafström, R & Roberg, K 2010, 'Fibronectin 1 is a potential biomarker for radioresistance in head and neck squamous cell carcinoma', Cancer Biology and Therapy, vol. 10, no. 12, pp. 1244-1251. https://doi.org/10.4161/cbt.10.12.13432

Fibronectin 1 is a potential biomarker for radioresistance in head and neck squamous cell carcinoma. / Jerhammer, F. (Corresponding Author); Ceder, R.; Garvin, S.; Grénman, R.; Grafström, Roland; Roberg, K.

In: Cancer Biology and Therapy, Vol. 10, No. 12, 2010, p. 1244-1251.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Ceder, R.

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AU - Grafström, Roland

AU - Roberg, K.

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AB - Radiotherapy remains the backbone of head and neck cancer therapy but response is sometimes impeded by tumor radioresistance. Identifying predictive biomarkers of radiotherapy response is a crucial step towards personalized therapy. The aim of this study was to explore gene expression data in search of biomarkers predictive of the response to radiotherapy in head and neck squamous cell carcinoma (HNSCC).Microarray analysis was performed on five cell lines with various intrinsic radiosensitivity, selected from a panel of 29 HNSCC cell lines. The bioinformatics approach included Gene Ontology (GO) enrichment profiling and Ingenuity Pathway Analysis (IPA). The GO-analysis detected 16 deregulated categories from which development, receptor activity, and extracellular region represented the largest groups. Fourteen hub genes (CEBPA, CEBPB, CTNNB1, FN1, MYC, MYCN, PLAU, SDC4, SERPINE1, SP1, TAF4B, THBS1, TP53 and VLDLR) were identified from the IPA network analysis. The hub genes in the highest ranked network, (FN1, SERPINE1, THBS1 and VLDLR) were further subjected to qPCR analysis in the complete panel of 29 cell lines. Of these genes, high FN1 expression associated to high intrinsic radiosensitivity (p=0.047).In conclusion, gene ontologies and hub genes of importance for intrinsic radiosensitivity were defined. The overall results suggest that FN1 should be explored as a potential novel biomarker for radioresistance.

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SN - 1538-4047

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