Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

Maija Vuoriluoto, Hannes Orelma (Corresponding Author), Meri Lundahl, Maryam Borghei, Orlando J. Rojas (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    14 Citations (Scopus)

    Abstract

    We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

    Original languageEnglish
    Pages (from-to)1803-1813
    Number of pages11
    JournalBiomacromolecules
    Volume18
    Issue number6
    DOIs
    Publication statusPublished - 24 Apr 2017
    MoE publication typeNot Eligible

    Fingerprint

    Hemoglobin
    Cellulose
    Hemoglobins
    Antibodies
    Quartz
    Fluorescein-5-isothiocyanate
    Hydrogel
    Surface plasmon resonance
    Bioactivity
    Hydrogels
    Amines
    Esters
    Microscopic examination
    Tensile strength
    Copolymers
    Crystals
    Fibers
    Lasers
    benzophenone

    Keywords

    • antibodies
    • bins
    • nanofibers
    • organic polymers
    • spinning (fibers)
    • surface plasmon resonance
    • tensile strength

    Cite this

    Vuoriluoto, Maija ; Orelma, Hannes ; Lundahl, Meri ; Borghei, Maryam ; Rojas, Orlando J. / Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils. In: Biomacromolecules. 2017 ; Vol. 18, No. 6. pp. 1803-1813.
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    title = "Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils",
    abstract = "We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80{\%} retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5{\%} of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.",
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    author = "Maija Vuoriluoto and Hannes Orelma and Meri Lundahl and Maryam Borghei and Rojas, {Orlando J.}",
    year = "2017",
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    language = "English",
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    Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils. / Vuoriluoto, Maija; Orelma, Hannes (Corresponding Author); Lundahl, Meri; Borghei, Maryam; Rojas, Orlando J. (Corresponding Author).

    In: Biomacromolecules, Vol. 18, No. 6, 24.04.2017, p. 1803-1813.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

    AU - Vuoriluoto, Maija

    AU - Orelma, Hannes

    AU - Lundahl, Meri

    AU - Borghei, Maryam

    AU - Rojas, Orlando J.

    PY - 2017/4/24

    Y1 - 2017/4/24

    N2 - We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

    AB - We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

    KW - antibodies

    KW - bins

    KW - nanofibers

    KW - organic polymers

    KW - spinning (fibers)

    KW - surface plasmon resonance

    KW - tensile strength

    UR - http://www.scopus.com/inward/record.url?scp=85020713311&partnerID=8YFLogxK

    U2 - 10.1021/acs.biomac.7b00256

    DO - 10.1021/acs.biomac.7b00256

    M3 - Article

    VL - 18

    SP - 1803

    EP - 1813

    JO - Biomacromolecules

    JF - Biomacromolecules

    SN - 1525-7797

    IS - 6

    ER -