Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

Maija Vuoriluoto, Hannes Orelma (Corresponding Author), Meri Lundahl, Maryam Borghei, Orlando J. Rojas (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

13 Citations (Scopus)

Abstract

We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

Original languageEnglish
Pages (from-to)1803-1813
Number of pages11
JournalBiomacromolecules
Volume18
Issue number6
DOIs
Publication statusPublished - 24 Apr 2017
MoE publication typeNot Eligible

Fingerprint

Hemoglobin
Cellulose
Hemoglobins
Antibodies
Quartz
Fluorescein-5-isothiocyanate
Hydrogel
Surface plasmon resonance
Bioactivity
Hydrogels
Amines
Esters
Microscopic examination
Tensile strength
Copolymers
Crystals
Fibers
Lasers
benzophenone

Keywords

  • antibodies
  • bins
  • nanofibers
  • organic polymers
  • spinning (fibers)
  • surface plasmon resonance
  • tensile strength

Cite this

Vuoriluoto, Maija ; Orelma, Hannes ; Lundahl, Meri ; Borghei, Maryam ; Rojas, Orlando J. / Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils. In: Biomacromolecules. 2017 ; Vol. 18, No. 6. pp. 1803-1813.
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abstract = "We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80{\%} retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5{\%} of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.",
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Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils. / Vuoriluoto, Maija; Orelma, Hannes (Corresponding Author); Lundahl, Meri; Borghei, Maryam; Rojas, Orlando J. (Corresponding Author).

In: Biomacromolecules, Vol. 18, No. 6, 24.04.2017, p. 1803-1813.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Filaments with Affinity Binding and Wet Strength Can Be Achieved by Spinning Bifunctional Cellulose Nanofibrils

AU - Vuoriluoto, Maija

AU - Orelma, Hannes

AU - Lundahl, Meri

AU - Borghei, Maryam

AU - Rojas, Orlando J.

PY - 2017/4/24

Y1 - 2017/4/24

N2 - We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

AB - We demonstrate benzophenone (BP) conjugation via amine-reactive esters onto oxidized cellulosic fibers that were used as precursors, after microfluidization, of photoactive cellulose nanofibrils (CNF). From these fibrils, cellulose I filaments were synthesized by hydrogel spinning in an antisolvent followed by fast biradical UV cross-linking. As a result, the wet BP-CNF filaments retained extensively the original dry strength (a remarkable 80% retention). Thus, the principal limitation of these emerging materials was overcome (the wet tensile strength is typically <0.5% of the value measured in dry conditions). Subsequently, antihuman hemoglobin (anti-Hb) antibodies were conjugated onto residual surface carboxyl groups, making the filaments bifunctional for their active groups and properties (wet strength and bioactivity). Optical (surface plasmon resonance) and electroacoustic (quartz crystal microgravimetry) measurements conducted with the bifunctional CNF indicated effective anti-Hb conjugation (2.4 mg m -2), endowing an excellent sensitivity toward Hb targets (1.7 ± 0.12 mg m -2) and negligible nonspecific binding. Thus, the anti-Hb biointerface was deployed on filaments that captured Hb efficiently from aqueous matrices (confocal laser microscopy of FITC-labeled antibodies). Significantly, the anti-Hb biointerface was suitable for regeneration, while its sensitivity and selectivity in affinity binding can be tailored by application of blocking copolymers. The developed bifunctional filaments based on nanocellulose offer great promise in detection and affinity binding built upon 1D systems, which can be engineered into other structures for rational use of material and space.

KW - antibodies

KW - bins

KW - nanofibers

KW - organic polymers

KW - spinning (fibers)

KW - surface plasmon resonance

KW - tensile strength

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U2 - 10.1021/acs.biomac.7b00256

DO - 10.1021/acs.biomac.7b00256

M3 - Article

VL - 18

SP - 1803

EP - 1813

JO - Biomacromolecules

JF - Biomacromolecules

SN - 1525-7797

IS - 6

ER -