Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation

Martin Nirschl, Anja Blüher, Christiane Erler, Beate Katzschner, Inger Vikholm-Lundin, Sanna Auer, Janos Vörös, Wolfgang Pompe, Matthias Schreiter, Michael Mertig (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

64 Citations (Scopus)

Abstract

We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm2/ng and a minimum detectable mass of ∼1 ng/cm2 were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.
Original languageEnglish
Pages (from-to)180-184
JournalSensors and Actuators A: Physical
Volume156
Issue number1
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fingerprint

Acoustic resonators
DNA
deoxyribonucleic acid
resonators
proteins
Proteins
Adsorption
acoustics
Viscoelasticity
Gold
Labels
adsorption
viscoelasticity
Electrodes
Kinetics
surface layers
dissipation
gold
shear
electrodes

Keywords

  • Film bulk acoustic resonator
  • Shear mode operation
  • Biosensors
  • DNA hybridisation
  • Bacterial surface layer proteins

Cite this

Nirschl, Martin ; Blüher, Anja ; Erler, Christiane ; Katzschner, Beate ; Vikholm-Lundin, Inger ; Auer, Sanna ; Vörös, Janos ; Pompe, Wolfgang ; Schreiter, Matthias ; Mertig, Michael. / Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation. In: Sensors and Actuators A: Physical. 2009 ; Vol. 156, No. 1. pp. 180-184.
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abstract = "We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm2/ng and a minimum detectable mass of ∼1 ng/cm2 were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.",
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Nirschl, M, Blüher, A, Erler, C, Katzschner, B, Vikholm-Lundin, I, Auer, S, Vörös, J, Pompe, W, Schreiter, M & Mertig, M 2009, 'Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation', Sensors and Actuators A: Physical, vol. 156, no. 1, pp. 180-184. https://doi.org/10.1016/j.sna.2009.02.021

Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation. / Nirschl, Martin; Blüher, Anja; Erler, Christiane; Katzschner, Beate; Vikholm-Lundin, Inger; Auer, Sanna; Vörös, Janos; Pompe, Wolfgang; Schreiter, Matthias; Mertig, Michael (Corresponding Author).

In: Sensors and Actuators A: Physical, Vol. 156, No. 1, 2009, p. 180-184.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Nirschl, Martin

AU - Blüher, Anja

AU - Erler, Christiane

AU - Katzschner, Beate

AU - Vikholm-Lundin, Inger

AU - Auer, Sanna

AU - Vörös, Janos

AU - Pompe, Wolfgang

AU - Schreiter, Matthias

AU - Mertig, Michael

PY - 2009

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KW - Shear mode operation

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KW - Bacterial surface layer proteins

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