Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation

Martin Nirschl; Anja Blüher; Christiane Erler; Beate Katzschner; Inger Vikholm-Lundin; Sanna Auer; Janos Vörös; Wolfgang Pompe; Matthias Schreiter; Michael Mertig

doi:10.1016/j.sna.2009.02.021

Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation

Martin Nirschl
, Anja Blüher
, Christiane Erler
, Beate Katzschner
, Inger Vikholm-Lundin
, Sanna Auer
, Janos Vörös
, Wolfgang Pompe
, Matthias Schreiter
, Michael Mertig^*

^*Corresponding author for this work

VTT Technical Research Centre of Finland

Siemens AG
Swiss Federal Institute of Technology in Zurich (ETH Zürich)
Technische Universität Dresden (TUD)

Research output: Contribution to journal › Article › Scientific › peer-review

78 Citations (Scopus)

Abstract

We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm²/ng and a minimum detectable mass of ∼1 ng/cm² were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.

Original language	English
Pages (from-to)	180-184
Journal	Sensors and Actuators A: Physical
Volume	156
Issue number	1
DOIs	https://doi.org/10.1016/j.sna.2009.02.021
Publication status	Published - 2009
MoE publication type	A1 Journal article-refereed

Keywords

Film bulk acoustic resonator
Shear mode operation
Biosensors
DNA hybridisation
Bacterial surface layer proteins

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10.1016/j.sna.2009.02.021

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title = "Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation",

abstract = "We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm²/ng and a minimum detectable mass of ∼1 ng/cm² were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.",

keywords = "Film bulk acoustic resonator, Shear mode operation, Biosensors, DNA hybridisation, Bacterial surface layer proteins",

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TY - JOUR

T1 - Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation

AU - Nirschl, Martin

AU - Blüher, Anja

AU - Erler, Christiane

AU - Katzschner, Beate

AU - Vikholm-Lundin, Inger

AU - Auer, Sanna

AU - Vörös, Janos

AU - Pompe, Wolfgang

AU - Schreiter, Matthias

AU - Mertig, Michael

PY - 2009

Y1 - 2009

N2 - We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm²/ng and a minimum detectable mass of ∼1 ng/cm² were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.

AB - We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of ∼2 kHz cm²/ng and a minimum detectable mass of ∼1 ng/cm² were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated.

KW - Film bulk acoustic resonator

KW - Shear mode operation

KW - Biosensors

KW - DNA hybridisation

KW - Bacterial surface layer proteins

U2 - 10.1016/j.sna.2009.02.021

DO - 10.1016/j.sna.2009.02.021

M3 - Article

SN - 0924-4247

VL - 156

SP - 180

EP - 184

JO - Sensors and Actuators A: Physical

JF - Sensors and Actuators A: Physical

IS - 1

ER -

Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation

Abstract

Keywords

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