Film formation and surface properties of enzymatically crosslinked casein films

Helka Juvonen (Corresponding Author), Maria Smolander, Harry Boer, Jaakko Pere, Johanna Buchert, Jouko Peltonen

Research output: Contribution to journalArticleScientificpeer-review

26 Citations (Scopus)

Abstract

The use of renewable materials as barrier material is currently intensively investigated. Biopolymers such as polysaccharides, lipids, and proteins have been studied as barrier materials. Protein‐based films often possess good gas barrier properties, but because of their hydrophilic nature the gas barrier properties are sensitive to humidity. The improvement of the properties of sodium caseinate barrier films in potential packaging applications was studied by investigating the effects of enzymatic treatment and plasticizer on the film properties. Oxidoreductases Trametes hirsuta laccase (ThL) and Trichoderma reesei tyrosinase (TrTyr) were compared with transglutaminase for crosslinking of the sodium caseinate molecules in the films and coatings. All of the studied enzymes were able to crosslink sodium caseinate. Film solubility tests, protein electrophoresis, contact angle measurements, and atomic force microscopy studies showed that TrTyr treatment results in sodium caseinate films and coatings with better overall properties compared to treatment with ThL.
Original languageEnglish
Pages (from-to)2205-2213
Number of pages9
JournalJournal of Applied Polymer Science
Volume119
Issue number4
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Casein
Caseins
Surface properties
Sodium
Laccase
Monophenol Monooxygenase
Gases
Proteins
Plasticizers
Coatings
Biopolymers
Transglutaminases
Polysaccharides
Angle measurement
Electrophoresis
Crosslinking
Lipids
Contact angle
Atomic force microscopy
Atmospheric humidity

Keywords

  • enzymes
  • crosslinking
  • proteins
  • films
  • atomic force microscopy (AFM)

Cite this

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title = "Film formation and surface properties of enzymatically crosslinked casein films",
abstract = "The use of renewable materials as barrier material is currently intensively investigated. Biopolymers such as polysaccharides, lipids, and proteins have been studied as barrier materials. Protein‐based films often possess good gas barrier properties, but because of their hydrophilic nature the gas barrier properties are sensitive to humidity. The improvement of the properties of sodium caseinate barrier films in potential packaging applications was studied by investigating the effects of enzymatic treatment and plasticizer on the film properties. Oxidoreductases Trametes hirsuta laccase (ThL) and Trichoderma reesei tyrosinase (TrTyr) were compared with transglutaminase for crosslinking of the sodium caseinate molecules in the films and coatings. All of the studied enzymes were able to crosslink sodium caseinate. Film solubility tests, protein electrophoresis, contact angle measurements, and atomic force microscopy studies showed that TrTyr treatment results in sodium caseinate films and coatings with better overall properties compared to treatment with ThL.",
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Film formation and surface properties of enzymatically crosslinked casein films. / Juvonen, Helka (Corresponding Author); Smolander, Maria; Boer, Harry; Pere, Jaakko; Buchert, Johanna; Peltonen, Jouko.

In: Journal of Applied Polymer Science, Vol. 119, No. 4, 2011, p. 2205-2213.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Film formation and surface properties of enzymatically crosslinked casein films

AU - Juvonen, Helka

AU - Smolander, Maria

AU - Boer, Harry

AU - Pere, Jaakko

AU - Buchert, Johanna

AU - Peltonen, Jouko

PY - 2011

Y1 - 2011

N2 - The use of renewable materials as barrier material is currently intensively investigated. Biopolymers such as polysaccharides, lipids, and proteins have been studied as barrier materials. Protein‐based films often possess good gas barrier properties, but because of their hydrophilic nature the gas barrier properties are sensitive to humidity. The improvement of the properties of sodium caseinate barrier films in potential packaging applications was studied by investigating the effects of enzymatic treatment and plasticizer on the film properties. Oxidoreductases Trametes hirsuta laccase (ThL) and Trichoderma reesei tyrosinase (TrTyr) were compared with transglutaminase for crosslinking of the sodium caseinate molecules in the films and coatings. All of the studied enzymes were able to crosslink sodium caseinate. Film solubility tests, protein electrophoresis, contact angle measurements, and atomic force microscopy studies showed that TrTyr treatment results in sodium caseinate films and coatings with better overall properties compared to treatment with ThL.

AB - The use of renewable materials as barrier material is currently intensively investigated. Biopolymers such as polysaccharides, lipids, and proteins have been studied as barrier materials. Protein‐based films often possess good gas barrier properties, but because of their hydrophilic nature the gas barrier properties are sensitive to humidity. The improvement of the properties of sodium caseinate barrier films in potential packaging applications was studied by investigating the effects of enzymatic treatment and plasticizer on the film properties. Oxidoreductases Trametes hirsuta laccase (ThL) and Trichoderma reesei tyrosinase (TrTyr) were compared with transglutaminase for crosslinking of the sodium caseinate molecules in the films and coatings. All of the studied enzymes were able to crosslink sodium caseinate. Film solubility tests, protein electrophoresis, contact angle measurements, and atomic force microscopy studies showed that TrTyr treatment results in sodium caseinate films and coatings with better overall properties compared to treatment with ThL.

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KW - crosslinking

KW - proteins

KW - films

KW - atomic force microscopy (AFM)

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SN - 0021-8995

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