Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods

Rami Mannila; Timo Pulli; Heikki Saari; Kirsi Tappura; Jussi Tuppurainen; Hannu Välimäki; Aimo Niskanen

doi:10.1117/12.728282

Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods

Rami Mannila, Timo Pulli, Heikki Saari, Kirsi Tappura, Jussi Tuppurainen, Hannu Välimäki, Aimo Niskanen

Labsystems Diagnostics Oy

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

1 Citation (Scopus)

Abstract

VTT Technical Research Centre of Finland has developed two reader prototypes for immunodiagnostic tests. VTT has also developed a one-step, homogeneous noncompetitive immunoassay for small analytes using recombinant antibodies and morphine as the model analyte. VTT developed reader for lateral flow test. Lateral flow test is a strip, which has a sample area and a detection area. In the sample area there are antibodies attached to gold or fluorescence particles, which are captured into the detection area, if a sample has a desired analyte. The concentration of the measured sample is then calculated from the fluorescence detection or color change. The second developed prototype reader is based on Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET). In this reader samples are put on microwell array. There are two fluorophores in each of the wells and emission of both fluorophores is measured. The sample concentration is calculated from these emission signals. The optimization of homogenous FRET assays for morphine was included to this project. The first results obtained with the TR-FRET reader prototype show that the sensitivity of the current morphine test is clearly adequate.

Original language	English
Title of host publication	Diagnostic Optical Spectroscopy in Biomedicine IV
Publisher	International Society for Optics and Photonics SPIE
ISBN (Electronic)	978-0-8194-6772-0
ISBN (Print)	978-0-8194-6771-3
DOIs	https://doi.org/10.1117/12.728282 https://doi.org/10.1364/ECBO.2007.6628_27
Publication status	Published - 2007
MoE publication type	A4 Article in a conference publication
Event	Diagnostic Optical Spectroscopy in Biomedicine IV - Munich, Germany Duration: 19 Jun 2007 → 21 Jun 2007

Publication series

Series	Proceedings of SPIE
Volume	6628
ISSN	0277-786X

Conference

Conference	Diagnostic Optical Spectroscopy in Biomedicine IV
Country/Territory	Germany
City	Munich
Period	19/06/07 → 21/06/07

Keywords

Bioanalytical systems
Fluorescence resonance energy transfer
Immunoassay
Test reader for fluorescence detection
Time-resolved fluorescence

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Mannila, R., Pulli, T., Saari, H., Tappura, K., Tuppurainen, J., Välimäki, H., & Niskanen, A. (2007). Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods. In Diagnostic Optical Spectroscopy in Biomedicine IV Article 066280Q International Society for Optics and Photonics SPIE. https://doi.org/10.1117/12.728282, https://doi.org/10.1364/ECBO.2007.6628_27

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title = "Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods",

abstract = "VTT Technical Research Centre of Finland has developed two reader prototypes for immunodiagnostic tests. VTT has also developed a one-step, homogeneous noncompetitive immunoassay for small analytes using recombinant antibodies and morphine as the model analyte. VTT developed reader for lateral flow test. Lateral flow test is a strip, which has a sample area and a detection area. In the sample area there are antibodies attached to gold or fluorescence particles, which are captured into the detection area, if a sample has a desired analyte. The concentration of the measured sample is then calculated from the fluorescence detection or color change. The second developed prototype reader is based on Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET). In this reader samples are put on microwell array. There are two fluorophores in each of the wells and emission of both fluorophores is measured. The sample concentration is calculated from these emission signals. The optimization of homogenous FRET assays for morphine was included to this project. The first results obtained with the TR-FRET reader prototype show that the sensitivity of the current morphine test is clearly adequate.",

keywords = "Bioanalytical systems, Fluorescence resonance energy transfer, Immunoassay, Test reader for fluorescence detection, Time-resolved fluorescence",

author = "Rami Mannila and Timo Pulli and Heikki Saari and Kirsi Tappura and Jussi Tuppurainen and Hannu V{\"a}lim{\"a}ki and Aimo Niskanen",

year = "2007",

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language = "English",

isbn = "978-0-8194-6771-3",

series = "Proceedings of SPIE",

publisher = "International Society for Optics and Photonics SPIE",

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note = "Diagnostic Optical Spectroscopy in Biomedicine IV ; Conference date: 19-06-2007 Through 21-06-2007",

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Mannila, R , Pulli, T , Saari, H , Tappura, K, Tuppurainen, J, Välimäki, H & Niskanen, A 2007, Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods. in Diagnostic Optical Spectroscopy in Biomedicine IV., 066280Q, International Society for Optics and Photonics SPIE, Proceedings of SPIE, vol. 6628, Diagnostic Optical Spectroscopy in Biomedicine IV, Munich, Bavaria, Germany, 19/06/07. https://doi.org/10.1117/12.728282, https://doi.org/10.1364/ECBO.2007.6628_27

Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods. / Mannila, Rami ; Pulli, Timo ; Saari, Heikki et al.
Diagnostic Optical Spectroscopy in Biomedicine IV. International Society for Optics and Photonics SPIE, 2007. 066280Q (Proceedings of SPIE, Vol. 6628).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

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AU - Pulli, Timo

AU - Saari, Heikki

AU - Tappura, Kirsi

AU - Tuppurainen, Jussi

AU - Välimäki, Hannu

AU - Niskanen, Aimo

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AB - VTT Technical Research Centre of Finland has developed two reader prototypes for immunodiagnostic tests. VTT has also developed a one-step, homogeneous noncompetitive immunoassay for small analytes using recombinant antibodies and morphine as the model analyte. VTT developed reader for lateral flow test. Lateral flow test is a strip, which has a sample area and a detection area. In the sample area there are antibodies attached to gold or fluorescence particles, which are captured into the detection area, if a sample has a desired analyte. The concentration of the measured sample is then calculated from the fluorescence detection or color change. The second developed prototype reader is based on Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET). In this reader samples are put on microwell array. There are two fluorophores in each of the wells and emission of both fluorophores is measured. The sample concentration is calculated from these emission signals. The optimization of homogenous FRET assays for morphine was included to this project. The first results obtained with the TR-FRET reader prototype show that the sensitivity of the current morphine test is clearly adequate.

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KW - Fluorescence resonance energy transfer

KW - Immunoassay

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T3 - Proceedings of SPIE

BT - Diagnostic Optical Spectroscopy in Biomedicine IV

PB - International Society for Optics and Photonics SPIE

T2 - Diagnostic Optical Spectroscopy in Biomedicine IV

Y2 - 19 June 2007 through 21 June 2007

ER -

Mannila R , Pulli T , Saari H , Tappura K, Tuppurainen J, Välimäki H et al. Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods. In Diagnostic Optical Spectroscopy in Biomedicine IV. International Society for Optics and Photonics SPIE. 2007. 066280Q. (Proceedings of SPIE, Vol. 6628). doi: 10.1117/12.728282, 10.1364/ECBO.2007.6628_27

Fluorescence-based fast diagnostics platform for the direct and indirect immunodiagnostic analysis methods

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Keywords

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