TY - JOUR
T1 - Fluorescence study of the effect of the oxidized phospholipids on amyloid fibril formation by the apolipoprotein A-I N-terminal fragment
AU - Vus, Kateryna
AU - Girych, Mykhailo
AU - Trusova, Valeriya
AU - Gorbenko, Galyna
AU - Kinnunen, Paavo
AU - Mizuguchi, Chiharu
AU - Saito, Hiroyuki
PY - 2017/11
Y1 - 2017/11
N2 - The effects of the oxidized phospholipids (oxPLs) on amyloid fibril formation by the apolipoprotein A-I variant 1-83/G26R have been investigated using Thioflavin T fluorescence assay. All types of the PoxnoPC assemblies (dispersions, micelles and lipid bilayer vesicles) induced retardation of amyloid nucleation and elongation and the enhancement of the 1-83/G26R fibrillization, although PazePC micelles completely prevented protein aggregation at low protein-to-lipid molar ratios. The ability of PazePC to inhibit 1-83/G26R aggregation was explained by the protein-lipid electrostatic interactions, which either stabilize the α-helical structure of the membrane-associated 1-83/G26R or facilitate the protein solubilization by the detergent micelles.
AB - The effects of the oxidized phospholipids (oxPLs) on amyloid fibril formation by the apolipoprotein A-I variant 1-83/G26R have been investigated using Thioflavin T fluorescence assay. All types of the PoxnoPC assemblies (dispersions, micelles and lipid bilayer vesicles) induced retardation of amyloid nucleation and elongation and the enhancement of the 1-83/G26R fibrillization, although PazePC micelles completely prevented protein aggregation at low protein-to-lipid molar ratios. The ability of PazePC to inhibit 1-83/G26R aggregation was explained by the protein-lipid electrostatic interactions, which either stabilize the α-helical structure of the membrane-associated 1-83/G26R or facilitate the protein solubilization by the detergent micelles.
UR - http://www.scopus.com/inward/record.url?scp=85029880935&partnerID=8YFLogxK
U2 - 10.1016/j.cplett.2017.09.037
DO - 10.1016/j.cplett.2017.09.037
M3 - Article
SN - 0009-2614
VL - 688
SP - 1
EP - 6
JO - Chemical Physics Letters
JF - Chemical Physics Letters
ER -