Fluorimetric oxygen sensor for in vitro cell models

Hannu Välimäki, Joose Kreutzer, Jarmo Verho, Kirsi Tappura, Jukka Lekkala

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025%), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.
Original languageEnglish
Pages (from-to)610-613
JournalProcedia Engineering
Volume168
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed
Event30th Anniversary Eurosensors Conference, EUROSENSORS 2016 - Budapest, Hungary
Duration: 4 Sep 20167 Sep 2016
https://www.sciencedirect.com/journal/procedia-engineering/vol/168

Fingerprint

Oxygen sensors
Dyes
Oxygen
Polystyrenes
Sensors
Dissolved oxygen
Cytotoxicity
Photodiodes
Microfluidics
Optical microscopy
Light emitting diodes
Lenses
Signal to noise ratio
Fluorescence
Thin films
Monitoring
Polymers

Keywords

  • oxygen sensor
  • cell models
  • fluorimetric sensor
  • PtOEPK
  • thin polystryne films

Cite this

Välimäki, Hannu ; Kreutzer, Joose ; Verho, Jarmo ; Tappura, Kirsi ; Lekkala, Jukka. / Fluorimetric oxygen sensor for in vitro cell models. In: Procedia Engineering. 2016 ; Vol. 168. pp. 610-613.
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title = "Fluorimetric oxygen sensor for in vitro cell models",
abstract = "A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025{\%}), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.",
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Välimäki, H, Kreutzer, J, Verho, J, Tappura, K & Lekkala, J 2016, 'Fluorimetric oxygen sensor for in vitro cell models', Procedia Engineering, vol. 168, pp. 610-613. https://doi.org/10.1016/j.proeng.2016.11.226

Fluorimetric oxygen sensor for in vitro cell models. / Välimäki, Hannu; Kreutzer, Joose; Verho, Jarmo; Tappura, Kirsi; Lekkala, Jukka.

In: Procedia Engineering, Vol. 168, 2016, p. 610-613.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Fluorimetric oxygen sensor for in vitro cell models

AU - Välimäki, Hannu

AU - Kreutzer, Joose

AU - Verho, Jarmo

AU - Tappura, Kirsi

AU - Lekkala, Jukka

PY - 2016

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N2 - A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025%), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.

AB - A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025%), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.

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