Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 610-613 |
| Journal | Procedia Engineering |
| Volume | 168 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | A1 Journal article-refereed |
| Event | 30th Anniversary Eurosensors Conference, EUROSENSORS 2016 - Budapest, Hungary Duration: 4 Sep 2016 → 7 Sep 2016 https://www.sciencedirect.com/journal/procedia-engineering/vol/168 |
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Keywords
- oxygen sensor
- cell models
- fluorimetric sensor
- PtOEPK
- thin polystryne films
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Fluorimetric oxygen sensor for in vitro cell models. / Välimäki, Hannu; Kreutzer, Joose; Verho, Jarmo; Tappura, Kirsi; Lekkala, Jukka.
In: Procedia Engineering, Vol. 168, 2016, p. 610-613.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Fluorimetric oxygen sensor for in vitro cell models
AU - Välimäki, Hannu
AU - Kreutzer, Joose
AU - Verho, Jarmo
AU - Tappura, Kirsi
AU - Lekkala, Jukka
PY - 2016
Y1 - 2016
N2 - A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025%), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.
AB - A phase fluorimetric sensor targeted for the monitoring of dissolved oxygen concentration in microfluidic in vitro cell models is presented. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (PtOEPK) embedded in a thin polystyrene film. The simulated fluorescence emission characteristics show highly anisotropic distribution, and an efficient optical read-out based on a parabolic lens is presented. Experimental results show that the applied sensing scheme allows one to use thin films (<500 nm), dilute dye-polymer ratios (0.025%), low power LED excitation (< 1 mW), a simple phase locked photodiode read-out and yet achieve over 40 dB signal-to-noise ratio at 10 Hz data rate at physiologically relevant oxygen concentrations. These features are important in in vitro cell studies, as the potential cytotoxicity of the dyes and the sensing method (i.e. production of singlet oxygen) are reduced with low dye content and excitation power. In addition, thin and dilute polystyrene films are highly transparent and facilitate optical microscopy.
KW - oxygen sensor
KW - cell models
KW - fluorimetric sensor
KW - PtOEPK
KW - thin polystryne films
U2 - 10.1016/j.proeng.2016.11.226
DO - 10.1016/j.proeng.2016.11.226
M3 - Article
VL - 168
SP - 610
EP - 613
JO - Procedia Engineering
JF - Procedia Engineering
SN - 1877-7058
ER -