Abstract
This paper presents a phase fluorimetric sensor for the
monitoring of the oxygen concentration in in vitro cell
models. The sensing surface of the sensor consists of
oxygen sensitive fluorescent dyes (platinum(II)
octaethylporphyrinketone) embedded in a thin polystyrene
film. In order to optimize the optical read-out scheme of
the sensor, we carried out electromagnetic simulations of
a fluorescently doped polystyrene film deposited on a
glass-water interface. The simulation results showed
highly anisotropic angular emission distribution with the
maximum irradiance being at super critical angles, which
attracts tailored optical designs to maximize the
fluorescence collection efficiency. For this purpose, we
applied an efficient optical read-out scheme based on an
in-contact parabolic lens. The use of parabolic lens also
facilitates confocal total internal reflection excitation
from the substrate side. This makes the excitation
effective and insensitive to biofouling or other optical
changes in the sensing surface and, more importantly,
greatly reduces the amount of excitation power radiated
into the cell culture chamber. Experimental results show
that when applied together with phase fluorimetric
lifetime sensing, this optical scheme allows one to use
thin films (
Original language | English |
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Pages (from-to) | 738-746 |
Number of pages | 9 |
Journal | Sensors and Actuators B: Chemical |
Volume | 249 |
DOIs | |
Publication status | Published - 1 Jan 2017 |
MoE publication type | A1 Journal article-refereed |
Keywords
- fluorimetric oxygen sensor
- thin film fluorescence
- enhanced optical read-out
- in vitro cell models
- PtOEPK
- cardiac cells
- OtaNano