Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models

Hannu Välimäki, Jarmo Verho, Joose Kreutzer, Dhanesh Kattipparambil Rajan, Tomi Ryynänen, Mari Pekkanen-Mattila, Antti Ahola, Kirsi Tappura, Pasi Kallio, Jukka Lekkala

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)

Abstract

This paper presents a phase fluorimetric sensor for the monitoring of the oxygen concentration in in vitro cell models. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (platinum(II) octaethylporphyrinketone) embedded in a thin polystyrene film. In order to optimize the optical read-out scheme of the sensor, we carried out electromagnetic simulations of a fluorescently doped polystyrene film deposited on a glass-water interface. The simulation results showed highly anisotropic angular emission distribution with the maximum irradiance being at super critical angles, which attracts tailored optical designs to maximize the fluorescence collection efficiency. For this purpose, we applied an efficient optical read-out scheme based on an in-contact parabolic lens. The use of parabolic lens also facilitates confocal total internal reflection excitation from the substrate side. This makes the excitation effective and insensitive to biofouling or other optical changes in the sensing surface and, more importantly, greatly reduces the amount of excitation power radiated into the cell culture chamber. Experimental results show that when applied together with phase fluorimetric lifetime sensing, this optical scheme allows one to use thin films (
Original languageEnglish
Pages (from-to)738-746
Number of pages9
JournalSensors and Actuators B: Chemical
Volume249
DOIs
Publication statusPublished - 1 Jan 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Oxygen sensors
Polystyrenes
Lenses
sensors
polystyrene
Sensors
oxygen
cells
lenses
Oxygen
excitation
Biofouling
Optical design
Platinum
Fluorescent Dyes
Cell culture
irradiance
platinum
Dyes
simulation

Keywords

  • fluorimetric oxygen sensor
  • thin film fluorescence
  • enhanced optical read-out
  • in vitro cell models
  • PtOEPK
  • cardiac cells

Cite this

Välimäki, H., Verho, J., Kreutzer, J., Kattipparambil Rajan, D., Ryynänen, T., Pekkanen-Mattila, M., ... Lekkala, J. (2017). Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models. Sensors and Actuators B: Chemical, 249, 738-746. https://doi.org/10.1016/j.snb.2017.04.182
Välimäki, Hannu ; Verho, Jarmo ; Kreutzer, Joose ; Kattipparambil Rajan, Dhanesh ; Ryynänen, Tomi ; Pekkanen-Mattila, Mari ; Ahola, Antti ; Tappura, Kirsi ; Kallio, Pasi ; Lekkala, Jukka. / Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models. In: Sensors and Actuators B: Chemical. 2017 ; Vol. 249. pp. 738-746.
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Välimäki, H, Verho, J, Kreutzer, J, Kattipparambil Rajan, D, Ryynänen, T, Pekkanen-Mattila, M, Ahola, A, Tappura, K, Kallio, P & Lekkala, J 2017, 'Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models', Sensors and Actuators B: Chemical, vol. 249, pp. 738-746. https://doi.org/10.1016/j.snb.2017.04.182

Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models. / Välimäki, Hannu; Verho, Jarmo; Kreutzer, Joose; Kattipparambil Rajan, Dhanesh; Ryynänen, Tomi; Pekkanen-Mattila, Mari; Ahola, Antti; Tappura, Kirsi; Kallio, Pasi; Lekkala, Jukka.

In: Sensors and Actuators B: Chemical, Vol. 249, 01.01.2017, p. 738-746.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Välimäki, Hannu

AU - Verho, Jarmo

AU - Kreutzer, Joose

AU - Kattipparambil Rajan, Dhanesh

AU - Ryynänen, Tomi

AU - Pekkanen-Mattila, Mari

AU - Ahola, Antti

AU - Tappura, Kirsi

AU - Kallio, Pasi

AU - Lekkala, Jukka

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N2 - This paper presents a phase fluorimetric sensor for the monitoring of the oxygen concentration in in vitro cell models. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (platinum(II) octaethylporphyrinketone) embedded in a thin polystyrene film. In order to optimize the optical read-out scheme of the sensor, we carried out electromagnetic simulations of a fluorescently doped polystyrene film deposited on a glass-water interface. The simulation results showed highly anisotropic angular emission distribution with the maximum irradiance being at super critical angles, which attracts tailored optical designs to maximize the fluorescence collection efficiency. For this purpose, we applied an efficient optical read-out scheme based on an in-contact parabolic lens. The use of parabolic lens also facilitates confocal total internal reflection excitation from the substrate side. This makes the excitation effective and insensitive to biofouling or other optical changes in the sensing surface and, more importantly, greatly reduces the amount of excitation power radiated into the cell culture chamber. Experimental results show that when applied together with phase fluorimetric lifetime sensing, this optical scheme allows one to use thin films (

AB - This paper presents a phase fluorimetric sensor for the monitoring of the oxygen concentration in in vitro cell models. The sensing surface of the sensor consists of oxygen sensitive fluorescent dyes (platinum(II) octaethylporphyrinketone) embedded in a thin polystyrene film. In order to optimize the optical read-out scheme of the sensor, we carried out electromagnetic simulations of a fluorescently doped polystyrene film deposited on a glass-water interface. The simulation results showed highly anisotropic angular emission distribution with the maximum irradiance being at super critical angles, which attracts tailored optical designs to maximize the fluorescence collection efficiency. For this purpose, we applied an efficient optical read-out scheme based on an in-contact parabolic lens. The use of parabolic lens also facilitates confocal total internal reflection excitation from the substrate side. This makes the excitation effective and insensitive to biofouling or other optical changes in the sensing surface and, more importantly, greatly reduces the amount of excitation power radiated into the cell culture chamber. Experimental results show that when applied together with phase fluorimetric lifetime sensing, this optical scheme allows one to use thin films (

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JF - Sensors and Actuators B: Chemical

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Välimäki H, Verho J, Kreutzer J, Kattipparambil Rajan D, Ryynänen T, Pekkanen-Mattila M et al. Fluorimetric oxygen sensor with an efficient optical read-out for in vitro cell models. Sensors and Actuators B: Chemical. 2017 Jan 1;249:738-746. https://doi.org/10.1016/j.snb.2017.04.182