Formation of protein-oligosaccharide conjugates by laccase and tyrosinase

Emilia Selinheimo (Corresponding Author), Piritta Lampila, Maija-Liisa Mattinen, Johanna Buchert

Research output: Contribution to journalArticleScientificpeer-review

101 Citations (Scopus)

Abstract

Proteins and certain carbohydrates contain phenolic moieties, which are potential sites for modification of the function of the biopolymers. In this study, the capability of two different fungal oxidative enzymes, laccase from Trametes hirsuta (ThL) and tyrosinase from Trichoderma reesei (TrT), to catalyze formation of hetero-cross-linking between tyrosine side chains of α-casein and phenolic acids of hydrolyzed oat spelt xylan (hOSX) was studied. Formation of reaction products was followed by size exclusion chromatography (SEC), fluorescence spectroscopy, and SDS-PAGE, using specific staining methods for proteins and protein−carbohydrate conjugates. ThL and TrT were observed to differ significantly in their ability to catalyze the formation of protein−carbohydrate conjugates or the linking of the small molecular weight phenolic compounds to α-casein. The efficiency of these enzymes to directly cross-link protein also differed notably. TrT was able to cross-link α-casein more efficiently than ThL. ThL-catalyzed casein cross-linking was significantly enhanced by ferulic acid, p-coumaric acid, and also hOSX. The main reaction products by ThL appeared to be phenolic acid-bridged α-caseins. Indications of hetero-cross-link formation between α-casein and hOSX by both oxidative enzymes could be visualized by glycoprotein-specific staining in the SDS-PAGE analysis, although ThL was observed to be more effective in the heteroconjugate formation than TrT.
Original languageEnglish
Pages (from-to)3118-3128
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number9
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Keywords

  • Laccase
  • tyrosinase
  • protein
  • xylan
  • phenolic acids
  • cross-linking

Fingerprint Dive into the research topics of 'Formation of protein-oligosaccharide conjugates by laccase and tyrosinase'. Together they form a unique fingerprint.

Cite this