Frozen-dough baking potential of psychrotolerant Saccharomyces species and derived hybrids

Frederico Magalhaes (Corresponding Author), Alex Calton, Raija-Liisa Heiniö, Brian Gibson

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)


Despite Saccharomyces cerevisiae being a synonym for baker’s yeast, the species does not perform well in all baking-related conditions. In particular, dough fermentation, or proofing, is compromised by the species’ sensitivity to the low and freezing temperatures that are often used in modern bakeries. Here, screening trials that included representatives of all known Saccharomyces species, showed that S. cerevisiae was generally the most sensitive member of the genus with respect to cold and freezing conditions. We hypothesized therefore that the superior cold tolerance of the non-S. cerevisiae yeast would enable their use as frozen-dough baking strains. To test this, the different yeast species were incorporated into doughs, flash frozen and kept in a frozen state for 14 days. During the proofing stage, dough development was lower in doughs that had been frozen, relative to fresh doughs. This reduction in fermentation performance was however most pronounced with S. cerevisiae. The psychrotolerant yeasts S. eubayanus, S. jurei and S. arboricola showed a strong capacity for post-freeze proofing in terms of dough development and duration of lag phase prior to fermentation. The superior proofing power of these species resulted in breads that were significantly softer and less dense than those prepared with S. cerevisiae. A sensory panel could distinguish the S. cerevisiae and non-S. cerevisiae breads based on their physical properties, but aroma and taste were unaffected by the species employed. To further improve frozen dough baking properties, S. eubayanus, S. jurei and S. arboricola were crossed with baker’s yeast through rare mating, and hybrids with improved proofing capacities in both fresh and frozen doughs relative to the parents were created. The use of S. jurei and S. arboricola in baking represents the first potential technological application of these species.
Original languageEnglish
Article number103640
Number of pages9
JournalFood Microbiology
Publication statusPublished - Apr 2021
MoE publication typeA1 Journal article-refereed


  • baking
  • frozen dough
  • saccharomyces eubayanus
  • saccharomyces jurei
  • saccharomyces arboricola
  • hybridization


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