Functional characterisation of genes involved in pyridine alkaloid biosynthesis in tobacco

Suvi T. Häkkinen, S. Tilleman, S. Swiatek, V. De Sutter, Heiko Rischer, I. Vanhoutte, H. Van Onckelen, P. Hilson, D. Inzé, Kirsi-Marja Oksman-Caldentey (Corresponding Author), A. Goossens

    Research output: Contribution to journalArticleScientificpeer-review

    46 Citations (Scopus)


    Although secondary metabolism in Nicotiana tabacum (L.) (tobacco) is rather well studied, many molecular aspects of the biosynthetic pathways and their regulation remain to be disclosed, even for prominent compounds such as nicotine and other pyridine alkaloids. To identify players in tobacco pyridine alkaloid biosynthesis a functional screen was performed, starting from a tobacco gene collection established previously by means of combined transcript profiling and metabolite analysis. First, full-length cDNA clones were isolated for 34 genes, corresponding to tobacco transcript tag sequences putatively associated with pyridine alkaloid metabolism. Full-length open reading frames were transferred to pCaMV35S-steered overexpression vectors. The effects of plant transformation with these expression cassettes on the accumulation of nicotine and other pyridine alkaloids were assessed in transgenic tobacco Bright-Yellow 2 (BY-2) cell suspensions and hairy root cultures. This screen identified potential catalysers of tobacco pyridine metabolism, amongst which a lysine decarboxylase-like gene and a GH3-like enzyme. Overexpression of the GH3-like enzyme, presumably involved in auxin homeostasis and designated NtNEG1 (Nicotiana tabacum Nicotine-Enhancing GH3 enzyme 1), increased nicotine levels in BY-2 hairy roots significantly. This study shows how functional genomics-based identification of genes potentially involved in biosynthetic pathways followed by systematic functional assays in plant cells can be used at large-scale to decipher plant metabolic networks at the molecular level.
    Original languageEnglish
    Pages (from-to)2773-2785
    Issue number22-24
    Publication statusPublished - 2007
    MoE publication typeNot Eligible
    Event50 Years of the Phytochemical Society of Europe. Highlights in the Evolution of Phytochemistry - Cambridge, United Kingdom
    Duration: 11 Apr 200714 Apr 2007


    • nicotiana tabacum
    • solanaceae
    • tobacco
    • functional genomics
    • pyridine alkaloids
    • nicotine
    • lysine decarboxylase
    • GH3-like enzyme
    • jasmonate
    • auxin conjugates
    • cDNA-AFLP
    • metabolites
    • metabolomics


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