Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

Luis M. Bimbo (Corresponding Author), Ermei Mäkilä, Janne Raula, Timo Laaksonen, Päivi Laaksonen, Katharina Strommer, Esko I. Kauppinen, Jarno Salonen, Markus Linder, Jouni Hirvonen, Hélder A. Santos (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

50 Citations (Scopus)

Abstract

Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.
Original languageEnglish
Pages (from-to)9089-9099
Number of pages11
JournalBiomaterials
Volume32
Issue number34
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fingerprint

Porous silicon
Silicon
Hydrophobicity
Proteins
Biocompatibility
Permeation
Coatings
Dissolution
Hydrophobic and Hydrophilic Interactions
Pharmaceutical Preparations
HT29 Cells
Fungal Proteins
Caco-2 Cells
Surface Properties
Monolayers
Assays

Keywords

  • Silicon
  • microparticle
  • hydrophobin
  • biocompatibility
  • drug release

Cite this

Bimbo, L. M., Mäkilä, E., Raula, J., Laaksonen, T., Laaksonen, P., Strommer, K., ... Santos, H. A. (2011). Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. Biomaterials, 32(34), 9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011
Bimbo, Luis M. ; Mäkilä, Ermei ; Raula, Janne ; Laaksonen, Timo ; Laaksonen, Päivi ; Strommer, Katharina ; Kauppinen, Esko I. ; Salonen, Jarno ; Linder, Markus ; Hirvonen, Jouni ; Santos, Hélder A. / Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. In: Biomaterials. 2011 ; Vol. 32, No. 34. pp. 9089-9099.
@article{07bef525fae343d5a59022068d6faa4d,
title = "Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles",
abstract = "Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.",
keywords = "Silicon, microparticle, hydrophobin, biocompatibility, drug release",
author = "Bimbo, {Luis M.} and Ermei M{\"a}kil{\"a} and Janne Raula and Timo Laaksonen and P{\"a}ivi Laaksonen and Katharina Strommer and Kauppinen, {Esko I.} and Jarno Salonen and Markus Linder and Jouni Hirvonen and Santos, {H{\'e}lder A.}",
year = "2011",
doi = "10.1016/j.biomaterials.2011.08.011",
language = "English",
volume = "32",
pages = "9089--9099",
journal = "Biomaterials",
issn = "0142-9612",
publisher = "Elsevier",
number = "34",

}

Bimbo, LM, Mäkilä, E, Raula, J, Laaksonen, T, Laaksonen, P, Strommer, K, Kauppinen, EI, Salonen, J, Linder, M, Hirvonen, J & Santos, HA 2011, 'Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles', Biomaterials, vol. 32, no. 34, pp. 9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011

Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. / Bimbo, Luis M. (Corresponding Author); Mäkilä, Ermei; Raula, Janne; Laaksonen, Timo; Laaksonen, Päivi; Strommer, Katharina; Kauppinen, Esko I.; Salonen, Jarno; Linder, Markus; Hirvonen, Jouni; Santos, Hélder A. (Corresponding Author).

In: Biomaterials, Vol. 32, No. 34, 2011, p. 9089-9099.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

AU - Bimbo, Luis M.

AU - Mäkilä, Ermei

AU - Raula, Janne

AU - Laaksonen, Timo

AU - Laaksonen, Päivi

AU - Strommer, Katharina

AU - Kauppinen, Esko I.

AU - Salonen, Jarno

AU - Linder, Markus

AU - Hirvonen, Jouni

AU - Santos, Hélder A.

PY - 2011

Y1 - 2011

N2 - Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

AB - Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

KW - Silicon

KW - microparticle

KW - hydrophobin

KW - biocompatibility

KW - drug release

U2 - 10.1016/j.biomaterials.2011.08.011

DO - 10.1016/j.biomaterials.2011.08.011

M3 - Article

VL - 32

SP - 9089

EP - 9099

JO - Biomaterials

JF - Biomaterials

SN - 0142-9612

IS - 34

ER -

Bimbo LM, Mäkilä E, Raula J, Laaksonen T, Laaksonen P, Strommer K et al. Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. Biomaterials. 2011;32(34):9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011