Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

Luis M. Bimbo (Corresponding Author), Ermei Mäkilä, Janne Raula, Timo Laaksonen, Päivi Laaksonen, Katharina Strommer, Esko I. Kauppinen, Jarno Salonen, Markus Linder, Jouni Hirvonen, Hélder A. Santos (Corresponding Author)

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    Abstract

    Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.
    Original languageEnglish
    Pages (from-to)9089-9099
    Number of pages11
    JournalBiomaterials
    Volume32
    Issue number34
    DOIs
    Publication statusPublished - 2011
    MoE publication typeA1 Journal article-refereed

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    Keywords

    • Silicon
    • microparticle
    • hydrophobin
    • biocompatibility
    • drug release

    Cite this

    Bimbo, L. M., Mäkilä, E., Raula, J., Laaksonen, T., Laaksonen, P., Strommer, K., Kauppinen, E. I., Salonen, J., Linder, M., Hirvonen, J., & Santos, H. A. (2011). Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. Biomaterials, 32(34), 9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011