Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

Luis M. Bimbo (Corresponding Author), Ermei Mäkilä, Janne Raula, Timo Laaksonen, Päivi Laaksonen, Katharina Strommer, Esko I. Kauppinen, Jarno Salonen, Markus Linder, Jouni Hirvonen, Hélder A. Santos (Corresponding Author)

    Research output: Contribution to journalArticleScientificpeer-review

    52 Citations (Scopus)

    Abstract

    Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.
    Original languageEnglish
    Pages (from-to)9089-9099
    Number of pages11
    JournalBiomaterials
    Volume32
    Issue number34
    DOIs
    Publication statusPublished - 2011
    MoE publication typeA1 Journal article-refereed

    Fingerprint

    Porous silicon
    Silicon
    Hydrophobicity
    Proteins
    Biocompatibility
    Permeation
    Coatings
    Dissolution
    Hydrophobic and Hydrophilic Interactions
    Pharmaceutical Preparations
    HT29 Cells
    Fungal Proteins
    Caco-2 Cells
    Surface Properties
    Monolayers
    Assays

    Keywords

    • Silicon
    • microparticle
    • hydrophobin
    • biocompatibility
    • drug release

    Cite this

    Bimbo, L. M., Mäkilä, E., Raula, J., Laaksonen, T., Laaksonen, P., Strommer, K., ... Santos, H. A. (2011). Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. Biomaterials, 32(34), 9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011
    Bimbo, Luis M. ; Mäkilä, Ermei ; Raula, Janne ; Laaksonen, Timo ; Laaksonen, Päivi ; Strommer, Katharina ; Kauppinen, Esko I. ; Salonen, Jarno ; Linder, Markus ; Hirvonen, Jouni ; Santos, Hélder A. / Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. In: Biomaterials. 2011 ; Vol. 32, No. 34. pp. 9089-9099.
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    abstract = "Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.",
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    Bimbo, LM, Mäkilä, E, Raula, J, Laaksonen, T, Laaksonen, P, Strommer, K, Kauppinen, EI, Salonen, J, Linder, M, Hirvonen, J & Santos, HA 2011, 'Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles', Biomaterials, vol. 32, no. 34, pp. 9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011

    Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. / Bimbo, Luis M. (Corresponding Author); Mäkilä, Ermei; Raula, Janne; Laaksonen, Timo; Laaksonen, Päivi; Strommer, Katharina; Kauppinen, Esko I.; Salonen, Jarno; Linder, Markus; Hirvonen, Jouni; Santos, Hélder A. (Corresponding Author).

    In: Biomaterials, Vol. 32, No. 34, 2011, p. 9089-9099.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles

    AU - Bimbo, Luis M.

    AU - Mäkilä, Ermei

    AU - Raula, Janne

    AU - Laaksonen, Timo

    AU - Laaksonen, Päivi

    AU - Strommer, Katharina

    AU - Kauppinen, Esko I.

    AU - Salonen, Jarno

    AU - Linder, Markus

    AU - Hirvonen, Jouni

    AU - Santos, Hélder A.

    PY - 2011

    Y1 - 2011

    N2 - Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

    AB - Porous silicon (PSi) particles have been widely used in modulating the dissolution rate of various types of drugs loaded within its mesopores. This material can be surface treated in order to vary its hydrophobicity and several other properties, such as drug loading degree and release rate. Hydrophobins are a family of self-assembling proteins of fungal origin which have the ability to form layers on hydrophobic materials. This type of protein layer can modify the characteristics and control the binding properties of the surface on which it assembles. In this study, we have developed a procedure to coat thermally hydrocarbonized-PSi microparticles with hydrophobin II (HFBII) in order to modify the particles’ hydrophobicity and to improve their biocompatibility, while maintaining intact the advantageous drug releasing properties of the PSi. The HFBII content adsorbed onto the particles was successfully quantified by a protein assay. Drug dissolution and permeation across Caco-2 cell monolayers were also conducted, together with viability studies in AGS, Caco-2 and HT-29 cells. The characterization and coating stability assessment showed that the HFBII-coating desorbs partially from the particles’ surface as the pH increases. The HFBII coating also improved the biocompatibility of the particles without compromising the enhanced drug permeation or release.

    KW - Silicon

    KW - microparticle

    KW - hydrophobin

    KW - biocompatibility

    KW - drug release

    U2 - 10.1016/j.biomaterials.2011.08.011

    DO - 10.1016/j.biomaterials.2011.08.011

    M3 - Article

    VL - 32

    SP - 9089

    EP - 9099

    JO - Biomaterials

    JF - Biomaterials

    SN - 0142-9612

    IS - 34

    ER -

    Bimbo LM, Mäkilä E, Raula J, Laaksonen T, Laaksonen P, Strommer K et al. Functional hydrophobin-coating of thermally hydrocarbonized porous silicon microparticles. Biomaterials. 2011;32(34):9089-9099. https://doi.org/10.1016/j.biomaterials.2011.08.011