GC/MS analysis of microbial metabolites from flavanols in an in vitro human colon model

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers or in condensed forms as proanthocyanidins (PA). Pure (+)-catechin, (-)-epicatechin and isolated PA fractions with different chain lengths were fermented with pooled human fecal microbiota in strictly anaerobic conditions. GC/MS analytical method was developed for the analysis of microbial metabolites. We will describe a GC/MS analytical method and the preceding sample preparation steps for the analysis of 11 microbial metabolites of (+)–catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. The sample preparation steps include sample extraction (wet or dry) with ethyl acetate and silylation with MSTFA. The fermentation and calibration samples are run in SIM mode and quantified using an internal standard. Different extraction methods and chromatographical methods are compared.
Original languageEnglish
Title of host publication17th International Mass Spectrometry Conference
Publication statusPublished - 2006
Event17th International Mass Spectrometry Conference - Prague, Czech Republic
Duration: 27 Aug 20061 Sep 2006

Conference

Conference17th International Mass Spectrometry Conference
CountryCzech Republic
CityPrague
Period27/08/061/09/06

Fingerprint

Catechin
Metabolites
Proanthocyanidins
Stilbenes
Lignans
Tannins
Phytochemicals
Chain length
Flavonoids
Fermentation
Monomers
Calibration
Derivatives

Keywords

  • acids
  • mass spectrometry
  • gas chromatography
  • metabolism
  • metabolites
  • phenols

Cite this

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title = "GC/MS analysis of microbial metabolites from flavanols in an in vitro human colon model",
abstract = "Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers or in condensed forms as proanthocyanidins (PA). Pure (+)-catechin, (-)-epicatechin and isolated PA fractions with different chain lengths were fermented with pooled human fecal microbiota in strictly anaerobic conditions. GC/MS analytical method was developed for the analysis of microbial metabolites. We will describe a GC/MS analytical method and the preceding sample preparation steps for the analysis of 11 microbial metabolites of (+)–catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. The sample preparation steps include sample extraction (wet or dry) with ethyl acetate and silylation with MSTFA. The fermentation and calibration samples are run in SIM mode and quantified using an internal standard. Different extraction methods and chromatographical methods are compared.",
keywords = "acids, mass spectrometry, gas chromatography, metabolism, metabolites, phenols",
author = "Ismo Mattila and Tuulikki Sepp{\"a}nen-Laakso and Anna-Marja Aura",
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booktitle = "17th International Mass Spectrometry Conference",

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Mattila, I, Seppänen-Laakso, T & Aura, A-M 2006, GC/MS analysis of microbial metabolites from flavanols in an in vitro human colon model. in 17th International Mass Spectrometry Conference. 17th International Mass Spectrometry Conference, Prague, Czech Republic, 27/08/06.

GC/MS analysis of microbial metabolites from flavanols in an in vitro human colon model. / Mattila, Ismo; Seppänen-Laakso, Tuulikki; Aura, Anna-Marja.

17th International Mass Spectrometry Conference. 2006.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - GC/MS analysis of microbial metabolites from flavanols in an in vitro human colon model

AU - Mattila, Ismo

AU - Seppänen-Laakso, Tuulikki

AU - Aura, Anna-Marja

PY - 2006

Y1 - 2006

N2 - Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers or in condensed forms as proanthocyanidins (PA). Pure (+)-catechin, (-)-epicatechin and isolated PA fractions with different chain lengths were fermented with pooled human fecal microbiota in strictly anaerobic conditions. GC/MS analytical method was developed for the analysis of microbial metabolites. We will describe a GC/MS analytical method and the preceding sample preparation steps for the analysis of 11 microbial metabolites of (+)–catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. The sample preparation steps include sample extraction (wet or dry) with ethyl acetate and silylation with MSTFA. The fermentation and calibration samples are run in SIM mode and quantified using an internal standard. Different extraction methods and chromatographical methods are compared.

AB - Dietary phenolic compounds are plant derived secondary metabolites, which can be divided into flavonoids, phenolic acids, stilbenes, tannins and lignans. Catechins can be found as monomers or in condensed forms as proanthocyanidins (PA). Pure (+)-catechin, (-)-epicatechin and isolated PA fractions with different chain lengths were fermented with pooled human fecal microbiota in strictly anaerobic conditions. GC/MS analytical method was developed for the analysis of microbial metabolites. We will describe a GC/MS analytical method and the preceding sample preparation steps for the analysis of 11 microbial metabolites of (+)–catechin and (-)-epicatechin. The compounds include e.g. hydroxyphenyl propionic and acetic acid derivatives. The sample preparation steps include sample extraction (wet or dry) with ethyl acetate and silylation with MSTFA. The fermentation and calibration samples are run in SIM mode and quantified using an internal standard. Different extraction methods and chromatographical methods are compared.

KW - acids

KW - mass spectrometry

KW - gas chromatography

KW - metabolism

KW - metabolites

KW - phenols

M3 - Conference abstract in proceedings

BT - 17th International Mass Spectrometry Conference

ER -