Gene amplifications in osteosarcoma: CGH microarray analysis

Jassu Atiye, Maija Wolf, Sippy Kaur, Outi Monni, Tom Bohling, Aarne Kivioja, Eva Tas, Massimo Serra, Maija Tarkkanen, Sakari Knuutila (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high‐resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes. Nineteen of the 22 cases had amplifications that on average spanned more than 1 Mb and contained more than 10 genes. Numerous regions of gain and loss were identified, and their boundaries were defined at high resolution. Novel amplicons were found at 14q11, 17q25, and 22q11–q13. Earlier‐known large amplified regions were detected at 12q11–q15, 8q24, 6p12–p13, and 17p11–p13 in 8, 6, 5, and 4 of the 22 samples, respectively. Amplification of 12q was observed more frequently (36% of the cases) than previously reported. Previously known small amplicons at 1p34–p36, 1q21, 19q13, and 21q22 were seen in at least three cases. Our results implicate TOM1L2 and CYP27B1 as having roles as novel targets for the 17p and 12q amplicons, respectively. Details (www.helsinki.fi/cmg) of the amplified genes in each amplicon provide valuable raw data for further in silico studies.
Original languageEnglish
Pages (from-to)158-163
JournalGenes, Chromosomes and Cancer
Volume42
Issue number2
DOIs
Publication statusPublished - 2005
MoE publication typeA1 Journal article-refereed

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Gene Amplification
Osteosarcoma
Microarray Analysis
25-Hydroxyvitamin D3 1-alpha-Hydroxylase
Genes
Comparative Genomic Hybridization
Gene Dosage
Oligonucleotide Array Sequence Analysis
Computer Simulation
Complementary DNA
Clone Cells

Cite this

Atiye, J., Wolf, M., Kaur, S., Monni, O., Bohling, T., Kivioja, A., ... Knuutila, S. (2005). Gene amplifications in osteosarcoma: CGH microarray analysis. Genes, Chromosomes and Cancer, 42(2), 158-163. https://doi.org/10.1002/gcc.20120
Atiye, Jassu ; Wolf, Maija ; Kaur, Sippy ; Monni, Outi ; Bohling, Tom ; Kivioja, Aarne ; Tas, Eva ; Serra, Massimo ; Tarkkanen, Maija ; Knuutila, Sakari. / Gene amplifications in osteosarcoma : CGH microarray analysis. In: Genes, Chromosomes and Cancer. 2005 ; Vol. 42, No. 2. pp. 158-163.
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Atiye, J, Wolf, M, Kaur, S, Monni, O, Bohling, T, Kivioja, A, Tas, E, Serra, M, Tarkkanen, M & Knuutila, S 2005, 'Gene amplifications in osteosarcoma: CGH microarray analysis', Genes, Chromosomes and Cancer, vol. 42, no. 2, pp. 158-163. https://doi.org/10.1002/gcc.20120

Gene amplifications in osteosarcoma : CGH microarray analysis. / Atiye, Jassu; Wolf, Maija; Kaur, Sippy; Monni, Outi; Bohling, Tom; Kivioja, Aarne; Tas, Eva; Serra, Massimo; Tarkkanen, Maija; Knuutila, Sakari (Corresponding Author).

In: Genes, Chromosomes and Cancer, Vol. 42, No. 2, 2005, p. 158-163.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - Gene amplifications in osteosarcoma

T2 - CGH microarray analysis

AU - Atiye, Jassu

AU - Wolf, Maija

AU - Kaur, Sippy

AU - Monni, Outi

AU - Bohling, Tom

AU - Kivioja, Aarne

AU - Tas, Eva

AU - Serra, Massimo

AU - Tarkkanen, Maija

AU - Knuutila, Sakari

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N2 - Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high‐resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes. Nineteen of the 22 cases had amplifications that on average spanned more than 1 Mb and contained more than 10 genes. Numerous regions of gain and loss were identified, and their boundaries were defined at high resolution. Novel amplicons were found at 14q11, 17q25, and 22q11–q13. Earlier‐known large amplified regions were detected at 12q11–q15, 8q24, 6p12–p13, and 17p11–p13 in 8, 6, 5, and 4 of the 22 samples, respectively. Amplification of 12q was observed more frequently (36% of the cases) than previously reported. Previously known small amplicons at 1p34–p36, 1q21, 19q13, and 21q22 were seen in at least three cases. Our results implicate TOM1L2 and CYP27B1 as having roles as novel targets for the 17p and 12q amplicons, respectively. Details (www.helsinki.fi/cmg) of the amplified genes in each amplicon provide valuable raw data for further in silico studies.

AB - Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high‐resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes. Nineteen of the 22 cases had amplifications that on average spanned more than 1 Mb and contained more than 10 genes. Numerous regions of gain and loss were identified, and their boundaries were defined at high resolution. Novel amplicons were found at 14q11, 17q25, and 22q11–q13. Earlier‐known large amplified regions were detected at 12q11–q15, 8q24, 6p12–p13, and 17p11–p13 in 8, 6, 5, and 4 of the 22 samples, respectively. Amplification of 12q was observed more frequently (36% of the cases) than previously reported. Previously known small amplicons at 1p34–p36, 1q21, 19q13, and 21q22 were seen in at least three cases. Our results implicate TOM1L2 and CYP27B1 as having roles as novel targets for the 17p and 12q amplicons, respectively. Details (www.helsinki.fi/cmg) of the amplified genes in each amplicon provide valuable raw data for further in silico studies.

U2 - 10.1002/gcc.20120

DO - 10.1002/gcc.20120

M3 - Article

VL - 42

SP - 158

EP - 163

JO - Genes, Chromosomes and Cancer

JF - Genes, Chromosomes and Cancer

SN - 1045-2257

IS - 2

ER -

Atiye J, Wolf M, Kaur S, Monni O, Bohling T, Kivioja A et al. Gene amplifications in osteosarcoma: CGH microarray analysis. Genes, Chromosomes and Cancer. 2005;42(2):158-163. https://doi.org/10.1002/gcc.20120