Gene expression engineering in fungi

Research output: Contribution to journalReview ArticleScientificpeer-review

Abstract

Fungi are a highly diverse group of microbial species that possess a plethora of biotechnologically useful metabolic and physiological properties. Important enablers for fungal biology studies and their biotechnological use are well-performing gene expression tools. Different types of gene expression tools exist; however, typically they are at best only functional in one or a few closely related species. This has hampered research and development of industrially relevant production systems. Here, we review operational principles and concepts of fungal gene expression tools. We present an overview on tools that utilize endogenous fungal promoters and modified hybrid expression systems composed of engineered promoters and transcription factors. Finally, we review synthetic expression tools that are functional across a broad range of fungal species.
Original languageEnglish
Pages (from-to)141-149
Number of pages9
JournalCurrent Opinion in Biotechnology
Volume59
DOIs
Publication statusPublished - Oct 2019
MoE publication typeA2 Review article in a scientific journal

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Fungi
Gene expression
Gene Expression
Fungal Genes
Transcription Factors
Transcription factors
Research

Cite this

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title = "Gene expression engineering in fungi",
abstract = "Fungi are a highly diverse group of microbial species that possess a plethora of biotechnologically useful metabolic and physiological properties. Important enablers for fungal biology studies and their biotechnological use are well-performing gene expression tools. Different types of gene expression tools exist; however, typically they are at best only functional in one or a few closely related species. This has hampered research and development of industrially relevant production systems. Here, we review operational principles and concepts of fungal gene expression tools. We present an overview on tools that utilize endogenous fungal promoters and modified hybrid expression systems composed of engineered promoters and transcription factors. Finally, we review synthetic expression tools that are functional across a broad range of fungal species.",
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Gene expression engineering in fungi. / Mojzita, Dominik; Rantasalo, Anssi; Jäntti, Jussi (Corresponding Author).

In: Current Opinion in Biotechnology, Vol. 59, 10.2019, p. 141-149.

Research output: Contribution to journalReview ArticleScientificpeer-review

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