Gene expression engineering in fungi

Dominik Mojzita; Anssi Rantasalo; Jussi Jäntti

doi:10.1016/j.copbio.2019.04.007

Gene expression engineering in fungi

Dominik Mojzita, Anssi Rantasalo, Jussi Jäntti (Corresponding Author)

BA3401 Production host engineering

Research output: Contribution to journal › Review Article › Scientific › peer-review

1 Citation (Scopus)

Abstract

Fungi are a highly diverse group of microbial species that possess a plethora of biotechnologically useful metabolic and physiological properties. Important enablers for fungal biology studies and their biotechnological use are well-performing gene expression tools. Different types of gene expression tools exist; however, typically they are at best only functional in one or a few closely related species. This has hampered research and development of industrially relevant production systems. Here, we review operational principles and concepts of fungal gene expression tools. We present an overview on tools that utilize endogenous fungal promoters and modified hybrid expression systems composed of engineered promoters and transcription factors. Finally, we review synthetic expression tools that are functional across a broad range of fungal species.

Original language	English
Pages (from-to)	141-149
Number of pages	9
Journal	Current Opinion in Biotechnology
Volume	59
DOIs	https://doi.org/10.1016/j.copbio.2019.04.007
Publication status	Published - Oct 2019
MoE publication type	A2 Review article in a scientific journal

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Mojzita, D., Rantasalo, A., & Jäntti, J. (2019). Gene expression engineering in fungi. Current Opinion in Biotechnology, 59, 141-149. https://doi.org/10.1016/j.copbio.2019.04.007

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