Abstract
Fungi are a highly diverse group of microbial species that possess a plethora of biotechnologically useful metabolic and physiological properties. Important enablers for fungal biology studies and their biotechnological use are well-performing gene expression tools. Different types of gene expression tools exist; however, typically they are at best only functional in one or a few closely related species. This has hampered research and development of industrially relevant production systems. Here, we review operational principles and concepts of fungal gene expression tools. We present an overview on tools that utilize endogenous fungal promoters and modified hybrid expression systems composed of engineered promoters and transcription factors. Finally, we review synthetic expression tools that are functional across a broad range of fungal species.
| Original language | English |
|---|---|
| Pages (from-to) | 141-149 |
| Journal | Current Opinion in Biotechnology |
| Volume | 59 |
| DOIs | |
| Publication status | Published - Oct 2019 |
| MoE publication type | A2 Review article in a scientific journal |
Funding
This material is based upon work supported by the Finnish Funding Agency for Technology and Innovation (TEKES) (Living factories project), and the Academy of Finland (MODUBIO – project 260957; SynBioPol – project 272550).