Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses

Kristine Kleivi, Guro E. Lind, Chieu B. Diep, Gunn I. Meling, Lin T. Brandal, Jahn M. Nesland, Ola Myklebost, Torleiv O. Rognum, Karl-Erik Giercksky, Rolf I. Skotheim, Ragnhild A. Lothe

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Abstract

Background Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses by use of DNA microarrays. Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel. Results Transcriptome profiles of colorectal cancer metastases independent of tumor site, as well as separate profiles associated with primary carcinomas, liver metastases, or peritoneal carcinomatoses, were assessed by use of Bayesian statistics. Gains of chromosome arm 5p are common in peritoneal carcinomatoses and several candidate genes (including PTGER4, SKP2, and ZNF622) mapping to this region were overexpressed in the tumors. Expression signatures stratified on TP53 mutation status were identified across all tumors regardless of stage. Furthermore, the gene expression levels for the in vivo tumors were compared with an in vitro model consisting of cell lines representing all three tumor stages established from one patient. Conclusion By statistical analysis of gene expression data from primary colorectal carcinomas, liver metastases, and carcinomatoses, we are able to identify genetic patterns associated with the different stages of tumorigenesis.
Original languageEnglish
Article number2
Number of pages16
JournalMolecular Cancer
Volume6
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Transcriptome
Colorectal Neoplasms
Neoplasm Metastasis
Carcinoma
Liver
Gene Expression
Neoplasms
Oligonucleotide Array Sequence Analysis
Carcinogenesis
Chromosomes
Cell Line
Mutation
Genes

Cite this

Kleivi, K., Lind, G. E., Diep, C. B., Meling, G. I., Brandal, L. T., Nesland, J. M., ... Lothe, R. A. (2007). Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses. Molecular Cancer, 6, [2]. https://doi.org/10.1186/1476-4598-6-2
Kleivi, Kristine ; Lind, Guro E. ; Diep, Chieu B. ; Meling, Gunn I. ; Brandal, Lin T. ; Nesland, Jahn M. ; Myklebost, Ola ; Rognum, Torleiv O. ; Giercksky, Karl-Erik ; Skotheim, Rolf I. ; Lothe, Ragnhild A. / Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses. In: Molecular Cancer. 2007 ; Vol. 6.
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title = "Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses",
abstract = "Background Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses by use of DNA microarrays. Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel. Results Transcriptome profiles of colorectal cancer metastases independent of tumor site, as well as separate profiles associated with primary carcinomas, liver metastases, or peritoneal carcinomatoses, were assessed by use of Bayesian statistics. Gains of chromosome arm 5p are common in peritoneal carcinomatoses and several candidate genes (including PTGER4, SKP2, and ZNF622) mapping to this region were overexpressed in the tumors. Expression signatures stratified on TP53 mutation status were identified across all tumors regardless of stage. Furthermore, the gene expression levels for the in vivo tumors were compared with an in vitro model consisting of cell lines representing all three tumor stages established from one patient. Conclusion By statistical analysis of gene expression data from primary colorectal carcinomas, liver metastases, and carcinomatoses, we are able to identify genetic patterns associated with the different stages of tumorigenesis.",
author = "Kristine Kleivi and Lind, {Guro E.} and Diep, {Chieu B.} and Meling, {Gunn I.} and Brandal, {Lin T.} and Nesland, {Jahn M.} and Ola Myklebost and Rognum, {Torleiv O.} and Karl-Erik Giercksky and Skotheim, {Rolf I.} and Lothe, {Ragnhild A.}",
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language = "English",
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Kleivi, K, Lind, GE, Diep, CB, Meling, GI, Brandal, LT, Nesland, JM, Myklebost, O, Rognum, TO, Giercksky, K-E, Skotheim, RI & Lothe, RA 2007, 'Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses', Molecular Cancer, vol. 6, 2. https://doi.org/10.1186/1476-4598-6-2

Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses. / Kleivi, Kristine; Lind, Guro E.; Diep, Chieu B.; Meling, Gunn I.; Brandal, Lin T.; Nesland, Jahn M.; Myklebost, Ola; Rognum, Torleiv O.; Giercksky, Karl-Erik; Skotheim, Rolf I.; Lothe, Ragnhild A.

In: Molecular Cancer, Vol. 6, 2, 2007.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Gene expression profiles of primary colorectal carcinomas, liver metastases, and carcinomatoses

AU - Kleivi, Kristine

AU - Lind, Guro E.

AU - Diep, Chieu B.

AU - Meling, Gunn I.

AU - Brandal, Lin T.

AU - Nesland, Jahn M.

AU - Myklebost, Ola

AU - Rognum, Torleiv O.

AU - Giercksky, Karl-Erik

AU - Skotheim, Rolf I.

AU - Lothe, Ragnhild A.

PY - 2007

Y1 - 2007

N2 - Background Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses by use of DNA microarrays. Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel. Results Transcriptome profiles of colorectal cancer metastases independent of tumor site, as well as separate profiles associated with primary carcinomas, liver metastases, or peritoneal carcinomatoses, were assessed by use of Bayesian statistics. Gains of chromosome arm 5p are common in peritoneal carcinomatoses and several candidate genes (including PTGER4, SKP2, and ZNF622) mapping to this region were overexpressed in the tumors. Expression signatures stratified on TP53 mutation status were identified across all tumors regardless of stage. Furthermore, the gene expression levels for the in vivo tumors were compared with an in vitro model consisting of cell lines representing all three tumor stages established from one patient. Conclusion By statistical analysis of gene expression data from primary colorectal carcinomas, liver metastases, and carcinomatoses, we are able to identify genetic patterns associated with the different stages of tumorigenesis.

AB - Background Despite the fact that metastases are the leading cause of colorectal cancer deaths, little is known about the underlying molecular changes in these advanced disease stages. Few have studied the overall gene expression levels in metastases from colorectal carcinomas, and so far, none has investigated the peritoneal carcinomatoses by use of DNA microarrays. Therefore, the aim of the present study is to investigate and compare the gene expression patterns of primary carcinomas (n = 18), liver metastases (n = 4), and carcinomatoses (n = 4), relative to normal samples from the large bowel. Results Transcriptome profiles of colorectal cancer metastases independent of tumor site, as well as separate profiles associated with primary carcinomas, liver metastases, or peritoneal carcinomatoses, were assessed by use of Bayesian statistics. Gains of chromosome arm 5p are common in peritoneal carcinomatoses and several candidate genes (including PTGER4, SKP2, and ZNF622) mapping to this region were overexpressed in the tumors. Expression signatures stratified on TP53 mutation status were identified across all tumors regardless of stage. Furthermore, the gene expression levels for the in vivo tumors were compared with an in vitro model consisting of cell lines representing all three tumor stages established from one patient. Conclusion By statistical analysis of gene expression data from primary colorectal carcinomas, liver metastases, and carcinomatoses, we are able to identify genetic patterns associated with the different stages of tumorigenesis.

U2 - 10.1186/1476-4598-6-2

DO - 10.1186/1476-4598-6-2

M3 - Article

VL - 6

JO - Molecular Cancer

JF - Molecular Cancer

SN - 1476-4598

M1 - 2

ER -