Generic method for attaching biomolecules via avidin-biotin complexes immobilized on films of regenerated and nanofibrillar cellulose

Hannes Orelma, Leena Sisko Johansson, Ilari Filpponen, Orlando J. Rojas, Janne Laine

Research output: Contribution to journalArticleScientificpeer-review

46 Citations (Scopus)

Abstract

We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated and nanofibrillar cellulose. The dynamics and extent of biomolecular attachment were monitored in situ by quartz crystal microbalance microgravimetry and ex situ via surface analyses with atomic force microscopy and X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) or TEMPO-oxidation significantly increases physisorption of avidins, which can be then covalently conjugated by using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride/N-hydroxysuccinimide (EDS/NHS) coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA) and antihuman immunoglobulin G (Biotin-anti-hIgG). Finally, we show that cellulose substrates carrying immobilized anti-hIgG are effective in detecting human immunoglobulin G (hIgG) from fluid matrices.

Original languageEnglish
Pages (from-to)2802-2810
JournalBiomacromolecules
Volume13
Issue number9
DOIs
Publication statusPublished - 10 Sep 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Avidin
Biomolecules
Biotin
Cellulose
Immunoglobulin G
Ethyldimethylaminopropyl Carbodiimide
Physisorption
Quartz crystal microbalances
Bovine Serum Albumin
Complexation
Energy dispersive spectroscopy
Atomic force microscopy
X ray photoelectron spectroscopy
Adsorption
Oxidation
Molecules
Fluids
Substrates

Cite this

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title = "Generic method for attaching biomolecules via avidin-biotin complexes immobilized on films of regenerated and nanofibrillar cellulose",
abstract = "We investigated the adsorption and chemical conjugation of avidin and its deglycosylated form, neutravidin, on films of regenerated and nanofibrillar cellulose. The dynamics and extent of biomolecular attachment were monitored in situ by quartz crystal microbalance microgravimetry and ex situ via surface analyses with atomic force microscopy and X-ray photoelectron spectroscopy. The installation of carboxyl groups on cellulose after modification with carboxymethylated cellulose (CMC) or TEMPO-oxidation significantly increases physisorption of avidins, which can be then covalently conjugated by using 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride/N-hydroxysuccinimide (EDS/NHS) coupling chemistries. The developed cellulose-avidin biointerfaces are able to scavenge biotinylated molecules from solution as demonstrated by successful surface complexation of biotinylated bovine serum albumin (Biotin-BSA) and antihuman immunoglobulin G (Biotin-anti-hIgG). Finally, we show that cellulose substrates carrying immobilized anti-hIgG are effective in detecting human immunoglobulin G (hIgG) from fluid matrices.",
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Generic method for attaching biomolecules via avidin-biotin complexes immobilized on films of regenerated and nanofibrillar cellulose. / Orelma, Hannes; Johansson, Leena Sisko; Filpponen, Ilari; Rojas, Orlando J.; Laine, Janne.

In: Biomacromolecules, Vol. 13, No. 9, 10.09.2012, p. 2802-2810.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Rojas, Orlando J.

AU - Laine, Janne

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