Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard; Ritva Verho; John Londesborough; Merja Penttilä

doi:10.1021/bk-2006-0921.ch014

Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard, Ritva Verho, John Londesborough, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Professional

1 Citation (Scopus)

Abstract

Recombinant Saccharomyces cerevisiae, able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields. Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol. The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. S. cerevisiae is unable to ferment pentoses but has been engineered to do so; however rates and yields are low. The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor. For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme. For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

Original language	English
Title of host publication	Feedstocks for the Future
Subtitle of host publication	Renewables for the Production of Chemicals and Materials
Editors	Joseph J. Bozell, Martin K. Patel
Place of Publication	Washington, D.C.
Publisher	American Chemical Society ACS
Chapter	14
Pages	184-192
ISBN (Electronic)	978-0-8412-2039-3
ISBN (Print)	978-0-8412-3934-0
DOIs	https://doi.org/10.1021/bk-2006-0921.ch014
Publication status	Published - 1 Dec 2006
MoE publication type	D2 Article in professional manuals or guides or professional information systems or text book material

Publication series

Series	ACS Symposium Series
Volume	921
ISSN	0097-6156

Keywords

Saccharomyces cerevisiae

Access to Document

10.1021/bk-2006-0921.ch014

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abstract = "Recombinant Saccharomyces cerevisiae, able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields. Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol. The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. S. cerevisiae is unable to ferment pentoses but has been engineered to do so; however rates and yields are low. The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor. For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme. For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.",

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Richard, P, Verho, R, Londesborough, J & Penttilä, M 2006, Genetic engineering of S. cerevisiae for pentose utilization. in JJ Bozell & MK Patel (eds), Feedstocks for the Future: Renewables for the Production of Chemicals and Materials. American Chemical Society ACS, Washington, D.C., ACS Symposium Series, vol. 921, pp. 184-192. https://doi.org/10.1021/bk-2006-0921.ch014

Genetic engineering of S. cerevisiae for pentose utilization. / Richard, Peter; Verho, Ritva; Londesborough, John et al.
Feedstocks for the Future: Renewables for the Production of Chemicals and Materials. ed. / Joseph J. Bozell; Martin K. Patel. Washington, D.C.: American Chemical Society ACS, 2006. p. 184-192 (ACS Symposium Series, Vol. 921).

Research output: Chapter in Book/Report/Conference proceeding › Chapter or book article › Professional

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AU - Verho, Ritva

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Genetic engineering of S. cerevisiae for pentose utilization

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