Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard, Ritva Verho, John Londesborough, Merja Penttilä

    Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

    1 Citation (Scopus)

    Abstract

    Recombinant Saccharomyces cerevisiae, able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields. Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol. The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. S. cerevisiae is unable to ferment pentoses but has been engineered to do so; however rates and yields are low. The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor. For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme. For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

    Original languageEnglish
    Title of host publicationFeedstocks for the Future
    Subtitle of host publicationRenewables for the Production of Chemicals and Materials
    EditorsJoseph J. Bozell, Martin K. Patel
    Place of PublicationWashington, D.C.
    PublisherAmerican Chemical Society ACS
    Chapter14
    Pages184-192
    ISBN (Electronic)978-0-8412-2039-3
    ISBN (Print)978-0-8412-3934-0
    DOIs
    Publication statusPublished - 1 Dec 2006
    MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

    Publication series

    SeriesACS Symposium Series
    Volume921
    ISSN0097-6156

    Keywords

    • Saccharomyces cerevisiae

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