Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard, Ritva Verho, John Londesborough, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingChapter or book articleProfessional

1 Citation (Scopus)


Recombinant Saccharomyces cerevisiae, able to ferment the pentoses D-xylose and L-arabinose, was modified for improved fermentation rates and yields. Pentose fermentation is relevant when low cost raw materials such as plant hydrolysates are fermented to ethanol. The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. S. cerevisiae is unable to ferment pentoses but has been engineered to do so; however rates and yields are low. The imbalance of redox cofactors (excess NADP and NADH are produced) is considered a major limiting factor. For the L-arabinose fermentation we identified an NADH-dependent L-xylulose reductase replacing the previously known NADPH-dependent enzyme. For D-xylose fermentation we introduced an NADP-dependent glyceraldehyde 3-phospate dehydrogenase to regenerate NADPH.

Original languageEnglish
Title of host publicationFeedstocks for the Future
Subtitle of host publicationRenewables for the Production of Chemicals and Materials
EditorsJoseph J. Bozell, Martin K. Patel
Place of PublicationWashington, D.C.
PublisherAmerican Chemical Society ACS
ISBN (Electronic)978-0-8412-2039-3
ISBN (Print)978-0-8412-3934-0
Publication statusPublished - 1 Dec 2006
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

Publication series

SeriesACS Symposium Series


  • Saccharomyces cerevisiae

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    Richard, P., Verho, R., Londesborough, J., & Penttilä, M. (2006). Genetic engineering of S. cerevisiae for pentose utilization. In J. J. Bozell, & M. K. Patel (Eds.), Feedstocks for the Future: Renewables for the Production of Chemicals and Materials (pp. 184-192). American Chemical Society ACS. ACS Symposium Series, Vol.. 921