Genetic engineering of S. cerevisiae for pentose utilization

Peter Richard, Ritva Verho, Mikko Putkonen, John Londesborough, Merja Penttilä

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

Abstract

The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. The pentose catabolic pathways are relevant for microorganisms living on decaying plant material and also in biotechnology when cheap raw materials such as plant hydrolysates are fermented to ethanol. We recently identified the two missing genes in the fungal pathway for L-arabinose catabolism. The functional overexpression of all the genes of the pathway in S cerevisiae led to growth on L-arabinose and ethanol production under anaerobic conditions however at very low rates. Pentose, i.e. D-xylose and L-arabinose fermentation to ethanol with recombinant S. cerevisiae is slow and has low yield. One reason is that the catabolism of these pentoses through the corresponding fungal pathways creates an imbalance of redox cofactors. The process, although redox neutral, requires NADPH which must be regenerated in a separate process. To facilitate the NADPH regeneration, the recently discovered gene GDP1 coding for a fungal NADP GAPDH was expressed in a S. cerevisiae strain with the D-xylose pathway. Glucose 6-phosphate dehydrogenase is the main path for NADPH regeneration, however it causes futile CO2 production and creates a redox imbalance on the pathway for anaerobic fermentation to ethanol. The deletion of the corresponding gene, zwf1, in combination with overexpression of GDP1 could stimulate D-xylose fermentation with respect to rate and yield; i.e. less CO2 and xylitol were produced. Through redox engineering a yeast strain, which was mainly producing xylitol and CO2 from D-xylose, was converted to a strain producing mainly ethanol.
Original languageEnglish
Title of host publication1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003
Subtitle of host publicationAbstract Book
PublisherElsevier
Pages9-10
Publication statusPublished - 2003
Event1st FEMS Congress of European Microbiologists - Ljubljana, Slovenia
Duration: 29 Jun 20033 Jul 2003

Conference

Conference1st FEMS Congress of European Microbiologists
CountrySlovenia
CityLjubljana
Period29/06/033/07/03

Fingerprint

pentoses
xylose
genetic engineering
arabinose
NADP (coenzyme)
ethanol
xylitol
fermentation
metabolism
genes
gene deletion
glucose-6-phosphate 1-dehydrogenase
ethanol production
hydrolysates
anaerobic conditions
biotechnology
raw materials
engineering
yeasts
sugars

Cite this

Richard, P., Verho, R., Putkonen, M., Londesborough, J., & Penttilä, M. (2003). Genetic engineering of S. cerevisiae for pentose utilization. In 1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003: Abstract Book (pp. 9-10). Elsevier.
Richard, Peter ; Verho, Ritva ; Putkonen, Mikko ; Londesborough, John ; Penttilä, Merja. / Genetic engineering of S. cerevisiae for pentose utilization. 1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003: Abstract Book. Elsevier, 2003. pp. 9-10
@inbook{27aa679f2a1b4263bd22723c9a17df52,
title = "Genetic engineering of S. cerevisiae for pentose utilization",
abstract = "The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. The pentose catabolic pathways are relevant for microorganisms living on decaying plant material and also in biotechnology when cheap raw materials such as plant hydrolysates are fermented to ethanol. We recently identified the two missing genes in the fungal pathway for L-arabinose catabolism. The functional overexpression of all the genes of the pathway in S cerevisiae led to growth on L-arabinose and ethanol production under anaerobic conditions however at very low rates. Pentose, i.e. D-xylose and L-arabinose fermentation to ethanol with recombinant S. cerevisiae is slow and has low yield. One reason is that the catabolism of these pentoses through the corresponding fungal pathways creates an imbalance of redox cofactors. The process, although redox neutral, requires NADPH which must be regenerated in a separate process. To facilitate the NADPH regeneration, the recently discovered gene GDP1 coding for a fungal NADP GAPDH was expressed in a S. cerevisiae strain with the D-xylose pathway. Glucose 6-phosphate dehydrogenase is the main path for NADPH regeneration, however it causes futile CO2 production and creates a redox imbalance on the pathway for anaerobic fermentation to ethanol. The deletion of the corresponding gene, zwf1, in combination with overexpression of GDP1 could stimulate D-xylose fermentation with respect to rate and yield; i.e. less CO2 and xylitol were produced. Through redox engineering a yeast strain, which was mainly producing xylitol and CO2 from D-xylose, was converted to a strain producing mainly ethanol.",
author = "Peter Richard and Ritva Verho and Mikko Putkonen and John Londesborough and Merja Penttil{\"a}",
note = "NT Food Solutions CA2: BEL2 CA: BEL",
year = "2003",
language = "English",
pages = "9--10",
booktitle = "1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003",
publisher = "Elsevier",
address = "Netherlands",

}

Richard, P, Verho, R, Putkonen, M, Londesborough, J & Penttilä, M 2003, Genetic engineering of S. cerevisiae for pentose utilization. in 1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003: Abstract Book. Elsevier, pp. 9-10, 1st FEMS Congress of European Microbiologists, Ljubljana, Slovenia, 29/06/03.

Genetic engineering of S. cerevisiae for pentose utilization. / Richard, Peter; Verho, Ritva; Putkonen, Mikko; Londesborough, John; Penttilä, Merja.

1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003: Abstract Book. Elsevier, 2003. p. 9-10.

Research output: Chapter in Book/Report/Conference proceedingConference abstract in proceedingsScientific

TY - CHAP

T1 - Genetic engineering of S. cerevisiae for pentose utilization

AU - Richard, Peter

AU - Verho, Ritva

AU - Putkonen, Mikko

AU - Londesborough, John

AU - Penttilä, Merja

N1 - NT Food Solutions CA2: BEL2 CA: BEL

PY - 2003

Y1 - 2003

N2 - The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. The pentose catabolic pathways are relevant for microorganisms living on decaying plant material and also in biotechnology when cheap raw materials such as plant hydrolysates are fermented to ethanol. We recently identified the two missing genes in the fungal pathway for L-arabinose catabolism. The functional overexpression of all the genes of the pathway in S cerevisiae led to growth on L-arabinose and ethanol production under anaerobic conditions however at very low rates. Pentose, i.e. D-xylose and L-arabinose fermentation to ethanol with recombinant S. cerevisiae is slow and has low yield. One reason is that the catabolism of these pentoses through the corresponding fungal pathways creates an imbalance of redox cofactors. The process, although redox neutral, requires NADPH which must be regenerated in a separate process. To facilitate the NADPH regeneration, the recently discovered gene GDP1 coding for a fungal NADP GAPDH was expressed in a S. cerevisiae strain with the D-xylose pathway. Glucose 6-phosphate dehydrogenase is the main path for NADPH regeneration, however it causes futile CO2 production and creates a redox imbalance on the pathway for anaerobic fermentation to ethanol. The deletion of the corresponding gene, zwf1, in combination with overexpression of GDP1 could stimulate D-xylose fermentation with respect to rate and yield; i.e. less CO2 and xylitol were produced. Through redox engineering a yeast strain, which was mainly producing xylitol and CO2 from D-xylose, was converted to a strain producing mainly ethanol.

AB - The two most widespread pentose sugars in our biosphere are D-xylose and L-arabinose. The pentose catabolic pathways are relevant for microorganisms living on decaying plant material and also in biotechnology when cheap raw materials such as plant hydrolysates are fermented to ethanol. We recently identified the two missing genes in the fungal pathway for L-arabinose catabolism. The functional overexpression of all the genes of the pathway in S cerevisiae led to growth on L-arabinose and ethanol production under anaerobic conditions however at very low rates. Pentose, i.e. D-xylose and L-arabinose fermentation to ethanol with recombinant S. cerevisiae is slow and has low yield. One reason is that the catabolism of these pentoses through the corresponding fungal pathways creates an imbalance of redox cofactors. The process, although redox neutral, requires NADPH which must be regenerated in a separate process. To facilitate the NADPH regeneration, the recently discovered gene GDP1 coding for a fungal NADP GAPDH was expressed in a S. cerevisiae strain with the D-xylose pathway. Glucose 6-phosphate dehydrogenase is the main path for NADPH regeneration, however it causes futile CO2 production and creates a redox imbalance on the pathway for anaerobic fermentation to ethanol. The deletion of the corresponding gene, zwf1, in combination with overexpression of GDP1 could stimulate D-xylose fermentation with respect to rate and yield; i.e. less CO2 and xylitol were produced. Through redox engineering a yeast strain, which was mainly producing xylitol and CO2 from D-xylose, was converted to a strain producing mainly ethanol.

M3 - Conference abstract in proceedings

SP - 9

EP - 10

BT - 1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003

PB - Elsevier

ER -

Richard P, Verho R, Putkonen M, Londesborough J, Penttilä M. Genetic engineering of S. cerevisiae for pentose utilization. In 1st FEMS Congress of European Microbiologists. Ljubljana, Slovenia, 29 June - 3 July 2003: Abstract Book. Elsevier. 2003. p. 9-10