Genetic engineering of Trichoderma to produce strains with novel cellulase profiles

Anu Harkki, Arja Mäntylä, Merja Penttilä, Susanna Muttilainen, Rolf Bühler, Pirkko Suominen, Jonathan Knowles, Helena Nevalainen

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Abstract

Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,β-d-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-β-d-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalEnzyme and Microbial Technology
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Jan 1991
MoE publication typeA1 Journal article-refereed

Keywords

  • cellulases
  • gene inactivation
  • hypercellulolytic mutant
  • overexpression
  • strain improvement
  • Trichoderma reesei

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    Harkki, A., Mäntylä, A., Penttilä, M., Muttilainen, S., Bühler, R., Suominen, P., Knowles, J., & Nevalainen, H. (1991). Genetic engineering of Trichoderma to produce strains with novel cellulase profiles. Enzyme and Microbial Technology, 13(3), 227-233. https://doi.org/10.1016/0141-0229(91)90133-U