Genetic engineering of Trichoderma to produce strains with novel cellulase profiles

Anu Harkki, Arja Mäntylä, Merja Penttilä, Susanna Muttilainen, Rolf Bühler, Pirkko Suominen, Jonathan Knowles, Helena Nevalainen

Research output: Contribution to journalArticleScientificpeer-review

76 Citations (Scopus)

Abstract

Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,β-d-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-β-d-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized.

Original languageEnglish
Pages (from-to)227-233
Number of pages7
JournalEnzyme and Microbial Technology
Volume13
Issue number3
DOIs
Publication statusPublished - 1 Jan 1991
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulose 1,4-beta-Cellobiosidase
Genetic engineering
Trichoderma
Genetic Engineering
Cellulase
Genes
Glucans
Enzymes
Genetic Terminator Regions
Complementary DNA
Cellulases

Keywords

  • cellulases
  • gene inactivation
  • hypercellulolytic mutant
  • overexpression
  • strain improvement
  • Trichoderma reesei

Cite this

Harkki, A., Mäntylä, A., Penttilä, M., Muttilainen, S., Bühler, R., Suominen, P., ... Nevalainen, H. (1991). Genetic engineering of Trichoderma to produce strains with novel cellulase profiles. Enzyme and Microbial Technology, 13(3), 227-233. https://doi.org/10.1016/0141-0229(91)90133-U
Harkki, Anu ; Mäntylä, Arja ; Penttilä, Merja ; Muttilainen, Susanna ; Bühler, Rolf ; Suominen, Pirkko ; Knowles, Jonathan ; Nevalainen, Helena. / Genetic engineering of Trichoderma to produce strains with novel cellulase profiles. In: Enzyme and Microbial Technology. 1991 ; Vol. 13, No. 3. pp. 227-233.
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abstract = "Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,β-d-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-β-d-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized.",
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Harkki, A, Mäntylä, A, Penttilä, M, Muttilainen, S, Bühler, R, Suominen, P, Knowles, J & Nevalainen, H 1991, 'Genetic engineering of Trichoderma to produce strains with novel cellulase profiles', Enzyme and Microbial Technology, vol. 13, no. 3, pp. 227-233. https://doi.org/10.1016/0141-0229(91)90133-U

Genetic engineering of Trichoderma to produce strains with novel cellulase profiles. / Harkki, Anu; Mäntylä, Arja; Penttilä, Merja; Muttilainen, Susanna; Bühler, Rolf; Suominen, Pirkko; Knowles, Jonathan; Nevalainen, Helena.

In: Enzyme and Microbial Technology, Vol. 13, No. 3, 01.01.1991, p. 227-233.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Mäntylä, Arja

AU - Penttilä, Merja

AU - Muttilainen, Susanna

AU - Bühler, Rolf

AU - Suominen, Pirkko

AU - Knowles, Jonathan

AU - Nevalainen, Helena

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