Genetic engineering of Trichoderma to produce strains with novel cellulase profiles

Anu Harkki, Arja Mäntylä, Merja Penttilä, Susanna Muttilainen, Rolf Bühler, Pirkko Suominen, Jonathan Knowles, Helena Nevalainen

    Research output: Contribution to journalArticleScientificpeer-review

    77 Citations (Scopus)

    Abstract

    Genetic engineering has been used to modify the proportion of different cellulases produced by a hypercellulolytic Trichoderma reesei mutant strain. A general expression vector, pAMH110, containing the promoter and terminator sequences of the strongly expressed main cellobiohydrolase 1 (cbh1) gene was used to overexpress a cDNA coding for EGI, the major endoglucanase (1,4,β-d-glucan glucanohydrolase, EC 3.2.1.4). An in vitro modified cbh1 cDNA, incapable of coding for active enzyme, was used to inactivate the major cellobiohydrolase (1,4-β-d-glucan cellobiohydrolase, EC 3.2.1.91) gene. In this way, new strains producing elevated amounts of the specific endoglucanase 1 (EGI) and/or lacking the major cellobiohydrolase (CBHI) were produced, and these have been further characterized.

    Original languageEnglish
    Pages (from-to)227-233
    Number of pages7
    JournalEnzyme and Microbial Technology
    Volume13
    Issue number3
    DOIs
    Publication statusPublished - 1 Jan 1991
    MoE publication typeA1 Journal article-refereed

    Keywords

    • cellulases
    • gene inactivation
    • hypercellulolytic mutant
    • overexpression
    • strain improvement
    • Trichoderma reesei

    Fingerprint Dive into the research topics of 'Genetic engineering of Trichoderma to produce strains with novel cellulase profiles'. Together they form a unique fingerprint.

    Cite this