Genetic heterogeneity and functional properties of intestinal bifidobacteria

Jaana Mättö, Eija Malinen, Maija-Liisa Suihko, Minna Alander, Airi Palva, Maria Saarela

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Aims: The aim of the present study was to compare several molecular methods for the identification and genotyping of bifidobacteria, and further to investigate genetic heterogeneity and functional properties of bifidobacterial isolates from intestinal samples of Finnish adult subjects. Methods and Results: A total of 153 intestinal bifidobacterial isolates were included in initial screening and 34 isolates were further characterized. Identification results obtained with PCR-ELISA and ribotyping were well in accordance with each other, while randomly amplified polymorphic DNA (RAPD) gave tentative identification only to Bifidobacterium bifidum and to 65% of the B. longum isolates. The most commonly detected species were B. longum biotype longum followed by B. adolescentis and B. bifidum. In addition, B. animalis (lactis), B. angulatum and B. pseudocatenulatum were found. Ribotyping and pulsed-field gel electrophoresis (PFGE) proved to be discriminatory methods for bifidobacteria, but also RAPD revealed intraspecies heterogeneity. Besides two B. animalis (lactis) isolates with very close similarity to a commercially available probiotic strain, none of the intestinal isolates showed optimal survival in all tolerance (acid, bile and oxygen) or growth performance tests. Conclusions: Several species/strains of bifidobacteria simultaneously colonize the gastrointestinal tract of healthy Finnish adults and intestinal Bifidobacterium isolates were genetically heterogeneous. Functional properties of bifidobacteria were strain-dependent. Significance and Impact of the Study: Applicability of ribotyping with the automated RiboPrinter® System for identification and genotyping of bifidobacteria was shown in the present study.
Original languageEnglish
Pages (from-to)459-470
JournalJournal of Applied Microbiology
Volume97
Issue number3
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

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Bifidobacterium
Genetic Heterogeneity
Ribotyping
Sulfalene
Pulsed Field Gel Electrophoresis
DNA
Probiotics
Bile Acids and Salts
Gastrointestinal Tract
Enzyme-Linked Immunosorbent Assay
Oxygen
Polymerase Chain Reaction
Growth

Keywords

  • bifidobacteria
  • faecal
  • functional properties
  • heterogeneity
  • ribotyping

Cite this

Mättö, J., Malinen, E., Suihko, M-L., Alander, M., Palva, A., & Saarela, M. (2004). Genetic heterogeneity and functional properties of intestinal bifidobacteria. Journal of Applied Microbiology, 97(3), 459-470. https://doi.org/10.1111/j.1365-2672.2004.02340.x
Mättö, Jaana ; Malinen, Eija ; Suihko, Maija-Liisa ; Alander, Minna ; Palva, Airi ; Saarela, Maria. / Genetic heterogeneity and functional properties of intestinal bifidobacteria. In: Journal of Applied Microbiology. 2004 ; Vol. 97, No. 3. pp. 459-470.
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Mättö, J, Malinen, E, Suihko, M-L, Alander, M, Palva, A & Saarela, M 2004, 'Genetic heterogeneity and functional properties of intestinal bifidobacteria', Journal of Applied Microbiology, vol. 97, no. 3, pp. 459-470. https://doi.org/10.1111/j.1365-2672.2004.02340.x

Genetic heterogeneity and functional properties of intestinal bifidobacteria. / Mättö, Jaana; Malinen, Eija; Suihko, Maija-Liisa; Alander, Minna; Palva, Airi; Saarela, Maria.

In: Journal of Applied Microbiology, Vol. 97, No. 3, 2004, p. 459-470.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Genetic heterogeneity and functional properties of intestinal bifidobacteria

AU - Mättö, Jaana

AU - Malinen, Eija

AU - Suihko, Maija-Liisa

AU - Alander, Minna

AU - Palva, Airi

AU - Saarela, Maria

PY - 2004

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N2 - Aims: The aim of the present study was to compare several molecular methods for the identification and genotyping of bifidobacteria, and further to investigate genetic heterogeneity and functional properties of bifidobacterial isolates from intestinal samples of Finnish adult subjects. Methods and Results: A total of 153 intestinal bifidobacterial isolates were included in initial screening and 34 isolates were further characterized. Identification results obtained with PCR-ELISA and ribotyping were well in accordance with each other, while randomly amplified polymorphic DNA (RAPD) gave tentative identification only to Bifidobacterium bifidum and to 65% of the B. longum isolates. The most commonly detected species were B. longum biotype longum followed by B. adolescentis and B. bifidum. In addition, B. animalis (lactis), B. angulatum and B. pseudocatenulatum were found. Ribotyping and pulsed-field gel electrophoresis (PFGE) proved to be discriminatory methods for bifidobacteria, but also RAPD revealed intraspecies heterogeneity. Besides two B. animalis (lactis) isolates with very close similarity to a commercially available probiotic strain, none of the intestinal isolates showed optimal survival in all tolerance (acid, bile and oxygen) or growth performance tests. Conclusions: Several species/strains of bifidobacteria simultaneously colonize the gastrointestinal tract of healthy Finnish adults and intestinal Bifidobacterium isolates were genetically heterogeneous. Functional properties of bifidobacteria were strain-dependent. Significance and Impact of the Study: Applicability of ribotyping with the automated RiboPrinter® System for identification and genotyping of bifidobacteria was shown in the present study.

AB - Aims: The aim of the present study was to compare several molecular methods for the identification and genotyping of bifidobacteria, and further to investigate genetic heterogeneity and functional properties of bifidobacterial isolates from intestinal samples of Finnish adult subjects. Methods and Results: A total of 153 intestinal bifidobacterial isolates were included in initial screening and 34 isolates were further characterized. Identification results obtained with PCR-ELISA and ribotyping were well in accordance with each other, while randomly amplified polymorphic DNA (RAPD) gave tentative identification only to Bifidobacterium bifidum and to 65% of the B. longum isolates. The most commonly detected species were B. longum biotype longum followed by B. adolescentis and B. bifidum. In addition, B. animalis (lactis), B. angulatum and B. pseudocatenulatum were found. Ribotyping and pulsed-field gel electrophoresis (PFGE) proved to be discriminatory methods for bifidobacteria, but also RAPD revealed intraspecies heterogeneity. Besides two B. animalis (lactis) isolates with very close similarity to a commercially available probiotic strain, none of the intestinal isolates showed optimal survival in all tolerance (acid, bile and oxygen) or growth performance tests. Conclusions: Several species/strains of bifidobacteria simultaneously colonize the gastrointestinal tract of healthy Finnish adults and intestinal Bifidobacterium isolates were genetically heterogeneous. Functional properties of bifidobacteria were strain-dependent. Significance and Impact of the Study: Applicability of ribotyping with the automated RiboPrinter® System for identification and genotyping of bifidobacteria was shown in the present study.

KW - bifidobacteria

KW - faecal

KW - functional properties

KW - heterogeneity

KW - ribotyping

U2 - 10.1111/j.1365-2672.2004.02340.x

DO - 10.1111/j.1365-2672.2004.02340.x

M3 - Article

VL - 97

SP - 459

EP - 470

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 3

ER -