Genetically engineered hairy root cultures of Hyoscyamus senecionis and H. muticus: ploidy as a promising parameter in the metabolic engineering of tropane alkaloids

Esmaeil Dehghan, Darwin W. Reed, Patrick S. Covello, Zeinab Hasanpour, Javier Palazon, Kirsi-Marja Oksman-Caldentey, Farajollah Shahriari Ahmadi

    Research output: Contribution to journalArticleScientificpeer-review

    11 Citations (Scopus)

    Abstract

    Key message: Tetraploidy improves overexpression ofh6hand scopolamine production ofH. muticus,while inH. senecionis, pmtoverexpression and elicitation can be used as effective methods for increasing tropane alkaloids. Abstract: The effects of metabolic engineering in a polyploid context were studied by overexpression of h6h in the tetraploid hairy root cultures of H. muticus. Flow cytometry analysis indicated genetic stability in the majority of the clones, while only a few clones showed genetic instability. Among all the diploid and tetraploid clones, the highest level of h6h transgene expression and scopolamine accumulation was interestingly observed in the tetraploid clones of H. muticus. Therefore, metabolic engineering of the tropane biosynthetic pathway in polyploids is suggested as a potential system for increasing the production of tropane alkaloids. Transgenic hairy root cultures of Hyoscyamus senecionis were also established. While overexpression of pmt in H. senecionis was correlated with a sharp increase in hyoscyamine production, the h6h-overexpressing clones were not able to accumulate higher levels of scopolamine than the leaves of intact plants. Applying methyl jasmonate was followed by a sharp increase in the expression of pmt and a drop in the expression of tropinone reductase II (trII) which consequently resulted in the higher biosynthesis of hyoscyamine and total alkaloids in H. senecionis.
    Original languageEnglish
    Pages (from-to)1615-1626
    JournalPlant Cell Reports
    Volume36
    Issue number10
    DOIs
    Publication statusPublished - 1 Oct 2017
    MoE publication typeA1 Journal article-refereed

    Keywords

    • hairy roots
    • metabolic engineering
    • methyl jasmonate
    • tetraploidy
    • tropane alkaloids

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