Abstract
The invention refers to fungal cells, and especially to oleaginous fungal cells that have been genetically modified to produce enzymes of the pyruvate dehydrogenase bypass route to enhance their lipid production. Especially the cells are modified to over-express genes encoding pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD) and/or acetyl-CoA synthetase (ACS), optionally together with a gene encoding diacylglycerol acyltransferase (DAT), or to express genes encoding PDC together with ALD and/or ACS. Methods of producing lipids, biofuels and lubricants using the modified fungi are also disclosed as well as expression cassettes useful therein. A new enzyme having phosholipid: diacylglycerol acyltransferase (PDAT)activity it and a polynucleotide encoding it are also disclosed, which are useful in the lipid production. A recombinant Cryptococcus cell and its construction is described.
Patent family as of 27.9.2021
EP2585588 A2 20130501 EP20110738253 20110621
FI122886 B 20120831 FI20100005733 20100624
FI20105733 A 20111225 FI20100005733 20100624
FI20105733 A0 20100624 FI20100005733 20100624
SG186431 A1 20130130 SG20120094389 20110621
US2013102042 AA 20130425 US20110806281 20110621
US9518276 BB 20161213 US20110806281 20110621
US2017088842 AA 20170330 US20160374811 20161209
WO11161317 A2 20111229 WO2011FI50594 20110621
WO11161317 A3 20120223 WO2011FI50594 20110621
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| Original language | English |
|---|---|
| Patent number | US2013102042 |
| IPC | C12N 15/ 80 A I |
| Priority date | 21/06/11 |
| Publication status | Published - 25 Apr 2013 |
| MoE publication type | H1 Granted patent |