Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice

Jussi Joensuu (Corresponding Author), Mirkka Kotiaho, Teemu Teeri, Leena Valmu, Anna-Maria Nuutila, Kirsi-Marja Oksman-Caldentey, Viola Niklander-Teeri

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level endosperm-specific production (1% of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes.
Original languageEnglish
Pages (from-to)359-373
Number of pages15
JournalTransgenic Research
Volume15
Issue number3
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fingerprint

Bacterial Adhesins
Enterotoxigenic Escherichia coli
Endosperm
enterotoxigenic Escherichia coli
adhesins
fimbriae
Hordeum
Neutralizing Antibodies
neutralizing antibodies
endosperm
piglets
Swine
barley
mucosal immunity
oral vaccination
Mucosal Immunity
receptors
swine
antibodies
Enterocytes

Keywords

  • barley
  • enterotoxigenic Escherichia coli
  • F4 (K88) fimbriae
  • FaeG
  • N-glycosylation
  • piglet

Cite this

@article{4ba271ee90fd41d19fcdb00b6bb602c5,
title = "Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice",
abstract = "The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level endosperm-specific production (1{\%} of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes.",
keywords = "barley, enterotoxigenic Escherichia coli, F4 (K88) fimbriae, FaeG, N-glycosylation, piglet",
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year = "2006",
doi = "10.1007/s11248-006-0010-7",
language = "English",
volume = "15",
pages = "359--373",
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}

Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice. / Joensuu, Jussi (Corresponding Author); Kotiaho, Mirkka; Teeri, Teemu; Valmu, Leena; Nuutila, Anna-Maria; Oksman-Caldentey, Kirsi-Marja; Niklander-Teeri, Viola.

In: Transgenic Research, Vol. 15, No. 3, 2006, p. 359-373.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Glycosylated F4 (K88) fimbrial adhesin FaeG expressed in barley endosperm induces ETEC-neutralizing antibodies in mice

AU - Joensuu, Jussi

AU - Kotiaho, Mirkka

AU - Teeri, Teemu

AU - Valmu, Leena

AU - Nuutila, Anna-Maria

AU - Oksman-Caldentey, Kirsi-Marja

AU - Niklander-Teeri, Viola

PY - 2006

Y1 - 2006

N2 - The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level endosperm-specific production (1% of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes.

AB - The F4-positive enterotoxigenic Escherichia coli (ETEC) strains are a frequent cause of porcine post-weaning diarrhea. Orally administered F4 fimbriae or FaeG, the major subunit and adhesin of F4, induce a protective mucosal immune response in F4 receptor-positive piglets. Feed plants carrying immunogenic subunit proteins can offer great advantages for oral vaccination of domestic animals. Here, we describe high-level endosperm-specific production (1% of total soluble proteins) of FaeG in the crop plant barley. The endoplasmic reticulum-targeted recombinant endospermic FaeG (erFaeG) was shown to be heterogeneously glycosylated. The erFaeG showed resistance at digestive conditions simulating piglet gastric fluid. Glycosylation did not abolish the immunogenic character of the FaeG protein, since erFaeG was able to induce F4 fimbria-specific antibodies in mice. Biological activity of these anti-F4 antibodies was demonstrated in vitro by blocking the attachment of the F4+ ETEC to the F4 receptors present on porcine intestinal enterocytes.

KW - barley

KW - enterotoxigenic Escherichia coli

KW - F4 (K88) fimbriae

KW - FaeG

KW - N-glycosylation

KW - piglet

U2 - 10.1007/s11248-006-0010-7

DO - 10.1007/s11248-006-0010-7

M3 - Article

VL - 15

SP - 359

EP - 373

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 3

ER -