Graphene biosensor programming by genetically engineered receptor modules

Sanna Arpiainen, Markku Kainlauri, Miika Soikkeli, Katri Kurppa, Jussi Joensuu, Päivi Laaksonen, Mika Prunnila, M. Linder, Jouni Ahopelto

    Research output: Contribution to conferenceConference AbstractScientific


    Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies in high ionic strength buffers resembling physiological media.
    Original languageEnglish
    Publication statusPublished - 2014
    EventGraphene Week, 23 - 27 June 2014, Gothenburg, Sweden - Gothenburg, Sweden
    Duration: 23 Jun 201427 Jun 2014


    ConferenceGraphene Week, 23 - 27 June 2014, Gothenburg, Sweden


    • graphene
    • hydrophobin
    • fusion protein
    • biosensor


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