Graphene biosensor programming by genetically engineered receptor modules

Research output: Contribution to conferenceConference AbstractScientific

Abstract

Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies in high ionic strength buffers resembling physiological media.
Original languageEnglish
Publication statusPublished - 2014
EventGraphene Week, 23 - 27 June 2014, Gothenburg, Sweden - Gothenburg, Sweden
Duration: 23 Jun 201427 Jun 2014

Conference

ConferenceGraphene Week, 23 - 27 June 2014, Gothenburg, Sweden
CountrySweden
CityGothenburg
Period23/06/1427/06/14

Keywords

  • graphene
  • hydrophobin
  • fusion protein
  • biosensor

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    Arpiainen, S., Kainlauri, M., Soikkeli, M., Kurppa, K., Joensuu, J., Laaksonen, P., Prunnila, M., Linder, M., & Ahopelto, J. (2014). Graphene biosensor programming by genetically engineered receptor modules. Abstract from Graphene Week, 23 - 27 June 2014, Gothenburg, Sweden, Gothenburg, Sweden.