Biosensing devices based on nanostructures and graphene facilitate label-free detection with sensitivities beyond traditional methods. Specific biorecognition relies on selective immobilization of analytes on the close vicinity of the sensor surface. We demonstrate single-step functionalization of graphene biosensors by using surface-active hydrophobin proteins that have been biologically fused with a receptor moiety. The fusion proteins self-align to form a dense and specifically oriented receptor monolayer and can be easily detached from the surface for sensor re-programming. Detection sensitivities are in femtomolar range both for small charged peptide analytes and for large immunoglobulin antibodies in high ionic strength buffers resembling physiological media.
|Publication status||Published - 2014|
|Event||Graphene Week, 23 - 27 June 2014, Gothenburg, Sweden - Gothenburg, Sweden|
Duration: 23 Jun 2014 → 27 Jun 2014
|Conference||Graphene Week, 23 - 27 June 2014, Gothenburg, Sweden|
|Period||23/06/14 → 27/06/14|
- fusion protein