Graphene biosensor programming with genetically engineered fusion protein monolayers

Miika Soikkeli, Katri Kurppa, Markku Kainlauri, Sanna Arpiainen, Arja Paananen, David Gunnarsson, Jussi J. Joensuu, Päivi Laaksonen, Mika Prunnila, Markus B. Linder, Jouni Ahopelto (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

28 Citations (Scopus)

Abstract

We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented).
Original languageEnglish
Pages (from-to)8257-8264
JournalACS applied materials & interfaces
Volume8
Issue number12
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fingerprint

Graphite
Biosensors
Graphene
Monolayers
Fusion reactions
Proteins
Molecules
Sensors
Field effect transistors
Fungi
Anchors
Self assembly
Peptides
Immunoglobulins
Labels
Drying

Keywords

  • biosensor
  • Debye length
  • fusion protein
  • graphene
  • hydrophobin
  • self-assembly

Cite this

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title = "Graphene biosensor programming with genetically engineered fusion protein monolayers",
abstract = "We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented).",
keywords = "biosensor, Debye length, fusion protein, graphene, hydrophobin, self-assembly",
author = "Miika Soikkeli and Katri Kurppa and Markku Kainlauri and Sanna Arpiainen and Arja Paananen and David Gunnarsson and Joensuu, {Jussi J.} and P{\"a}ivi Laaksonen and Mika Prunnila and Linder, {Markus B.} and Jouni Ahopelto",
year = "2016",
doi = "10.1021/acsami.6b00123",
language = "English",
volume = "8",
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journal = "ACS applied materials & interfaces",
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publisher = "American Chemical Society",
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Graphene biosensor programming with genetically engineered fusion protein monolayers. / Soikkeli, Miika; Kurppa, Katri; Kainlauri, Markku; Arpiainen, Sanna; Paananen, Arja; Gunnarsson, David; Joensuu, Jussi J.; Laaksonen, Päivi; Prunnila, Mika; Linder, Markus B.; Ahopelto, Jouni (Corresponding Author).

In: ACS applied materials & interfaces, Vol. 8, No. 12, 2016, p. 8257-8264.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Graphene biosensor programming with genetically engineered fusion protein monolayers

AU - Soikkeli, Miika

AU - Kurppa, Katri

AU - Kainlauri, Markku

AU - Arpiainen, Sanna

AU - Paananen, Arja

AU - Gunnarsson, David

AU - Joensuu, Jussi J.

AU - Laaksonen, Päivi

AU - Prunnila, Mika

AU - Linder, Markus B.

AU - Ahopelto, Jouni

PY - 2016

Y1 - 2016

N2 - We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented).

AB - We demonstrate a label-free biosensor concept based on specific receptor modules, which provide immobilization and selectivity to the desired analyte molecules, and on charge sensing with a graphene field effect transistor. The receptor modules are fusion proteins in which small hydrophobin proteins act as the anchor to immobilize the receptor moiety. The functionalization of the graphene sensor is a single-step process based on directed self-assembly of the receptor modules on a hydrophobic surface. The modules are produced separately in fungi or plants and purified before use. The modules form a dense and well-oriented monolayer on the graphene transistor channel and the receptor module monolayer can be removed, and a new module monolayer with a different selectivity can be assembled in situ. The receptor module monolayers survive drying, showing that the functionalized devices can be stored and have a reasonable shelf life. The sensor is tested with small charged peptides and large immunoglobulin molecules. The measured sensitivities are in the femtomolar range, and the response is relatively fast, of the order of one second. (Graph Presented).

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