Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study

Mari C.W. Myhrstad (Corresponding Author), Vanessa D. de Mello, Ingrid Dahlman, Marjukka Kolehmainen, Jussi Paananen, Amanda Rundblad, Carsten Carlberg, Ole Kristoffer Olstad, Jussi Pihlajamäki, Kirsten B. Holven, Kjeld Hermansen, Lars O. Dragsted, Ingibjörg Gunnarsdottir, Lieselotte Cloetens, Matilda Ulmius Storm, Björn Åkesson, Fredrik Rosqvist, Janne Hukkanen, Karl Heinz Herzig, Ulf RisérusInga Thorsdottir, Kaisa S. Poutanen, Markku J. Savolainen, Ursula Schwab, Peter Arner, Matti Uusitupa, Stine M. Ulven

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

Original languageEnglish
Article number1801405
JournalMolecular Nutrition and Food Research
Volume63
Issue number13
Early online date9 Apr 2019
DOIs
Publication statusPublished - 1 Jul 2019
MoE publication typeA1 Journal article-refereed

Fingerprint

mononuclear leukocytes
Blood Cells
immune response
Gene Expression
gene expression
diet
metabolic syndrome
Down-Regulation
Diet
Gene Expression Profiling
Electron Transport
Transcriptome
Cell Adhesion
healthy diet
electron transport chain
transcriptomics
Cell Cycle
cell adhesion
transcriptome
Transcription Factors

Keywords

  • gene-expression
  • healthy Nordic diet
  • metabolic syndrome
  • peripheral blood mononuclear cells
  • transcriptome

Cite this

Myhrstad, Mari C.W. ; de Mello, Vanessa D. ; Dahlman, Ingrid ; Kolehmainen, Marjukka ; Paananen, Jussi ; Rundblad, Amanda ; Carlberg, Carsten ; Olstad, Ole Kristoffer ; Pihlajamäki, Jussi ; Holven, Kirsten B. ; Hermansen, Kjeld ; Dragsted, Lars O. ; Gunnarsdottir, Ingibjörg ; Cloetens, Lieselotte ; Storm, Matilda Ulmius ; Åkesson, Björn ; Rosqvist, Fredrik ; Hukkanen, Janne ; Herzig, Karl Heinz ; Risérus, Ulf ; Thorsdottir, Inga ; Poutanen, Kaisa S. ; Savolainen, Markku J. ; Schwab, Ursula ; Arner, Peter ; Uusitupa, Matti ; Ulven, Stine M. / Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study. In: Molecular Nutrition and Food Research. 2019 ; Vol. 63, No. 13.
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title = "Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study",
abstract = "Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.",
keywords = "gene-expression, healthy Nordic diet, metabolic syndrome, peripheral blood mononuclear cells, transcriptome",
author = "Myhrstad, {Mari C.W.} and {de Mello}, {Vanessa D.} and Ingrid Dahlman and Marjukka Kolehmainen and Jussi Paananen and Amanda Rundblad and Carsten Carlberg and Olstad, {Ole Kristoffer} and Jussi Pihlajam{\"a}ki and Holven, {Kirsten B.} and Kjeld Hermansen and Dragsted, {Lars O.} and Ingibj{\"o}rg Gunnarsdottir and Lieselotte Cloetens and Storm, {Matilda Ulmius} and Bj{\"o}rn {\AA}kesson and Fredrik Rosqvist and Janne Hukkanen and Herzig, {Karl Heinz} and Ulf Ris{\'e}rus and Inga Thorsdottir and Poutanen, {Kaisa S.} and Savolainen, {Markku J.} and Ursula Schwab and Peter Arner and Matti Uusitupa and Ulven, {Stine M.}",
year = "2019",
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language = "English",
volume = "63",
journal = "Molecular Nutrition and Food Research",
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Myhrstad, MCW, de Mello, VD, Dahlman, I, Kolehmainen, M, Paananen, J, Rundblad, A, Carlberg, C, Olstad, OK, Pihlajamäki, J, Holven, KB, Hermansen, K, Dragsted, LO, Gunnarsdottir, I, Cloetens, L, Storm, MU, Åkesson, B, Rosqvist, F, Hukkanen, J, Herzig, KH, Risérus, U, Thorsdottir, I, Poutanen, KS, Savolainen, MJ, Schwab, U, Arner, P, Uusitupa, M & Ulven, SM 2019, 'Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study', Molecular Nutrition and Food Research, vol. 63, no. 13, 1801405. https://doi.org/10.1002/mnfr.201801405

Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study. / Myhrstad, Mari C.W. (Corresponding Author); de Mello, Vanessa D.; Dahlman, Ingrid; Kolehmainen, Marjukka; Paananen, Jussi; Rundblad, Amanda; Carlberg, Carsten; Olstad, Ole Kristoffer; Pihlajamäki, Jussi; Holven, Kirsten B.; Hermansen, Kjeld; Dragsted, Lars O.; Gunnarsdottir, Ingibjörg; Cloetens, Lieselotte; Storm, Matilda Ulmius; Åkesson, Björn; Rosqvist, Fredrik; Hukkanen, Janne; Herzig, Karl Heinz; Risérus, Ulf; Thorsdottir, Inga; Poutanen, Kaisa S.; Savolainen, Markku J.; Schwab, Ursula; Arner, Peter; Uusitupa, Matti; Ulven, Stine M.

In: Molecular Nutrition and Food Research, Vol. 63, No. 13, 1801405, 01.07.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Healthy Nordic Diet Modulates the Expression of Genes Related to Mitochondrial Function and Immune Response in Peripheral Blood Mononuclear Cells from Subjects with Metabolic Syndrome–A SYSDIET Sub-Study

AU - Myhrstad, Mari C.W.

AU - de Mello, Vanessa D.

AU - Dahlman, Ingrid

AU - Kolehmainen, Marjukka

AU - Paananen, Jussi

AU - Rundblad, Amanda

AU - Carlberg, Carsten

AU - Olstad, Ole Kristoffer

AU - Pihlajamäki, Jussi

AU - Holven, Kirsten B.

AU - Hermansen, Kjeld

AU - Dragsted, Lars O.

AU - Gunnarsdottir, Ingibjörg

AU - Cloetens, Lieselotte

AU - Storm, Matilda Ulmius

AU - Åkesson, Björn

AU - Rosqvist, Fredrik

AU - Hukkanen, Janne

AU - Herzig, Karl Heinz

AU - Risérus, Ulf

AU - Thorsdottir, Inga

AU - Poutanen, Kaisa S.

AU - Savolainen, Markku J.

AU - Schwab, Ursula

AU - Arner, Peter

AU - Uusitupa, Matti

AU - Ulven, Stine M.

PY - 2019/7/1

Y1 - 2019/7/1

N2 - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

AB - Scope: To explore the effect of a healthy Nordic diet on the global transcriptome profile in peripheral blood mononuclear cells (PBMCs) of subjects with metabolic syndrome. Methods and results: Subjects with metabolic syndrome undergo a 18/24 week randomized intervention study comparing an isocaloric healthy Nordic diet with an average habitual Nordic diet served as control (SYSDIET study). Altogether, 68 participants are included. PBMCs are obtained before and after intervention and total RNA is subjected to global transcriptome analysis. 1302 probe sets are differentially expressed between the diet groups (p-value < 0.05). Twenty-five of these are significantly regulated (FDR q-value < 0.25) and are mainly involved in mitochondrial function, cell growth, and cell adhesion. The list of 1302 regulated probe sets is subjected to functional analyses. Pathways and processes involved in the mitochondrial electron transport chain, immune response, and cell cycle are downregulated in the healthy Nordic diet group. In addition, gene transcripts with common motifs for 42 transcription factors, including NFR1, NFR2, and NF-κB, are downregulated in the healthy Nordic diet group. Conclusion: These results suggest that benefits of a healthy diet may be mediated by improved mitochondrial function and reduced inflammation.

KW - gene-expression

KW - healthy Nordic diet

KW - metabolic syndrome

KW - peripheral blood mononuclear cells

KW - transcriptome

UR - http://www.scopus.com/inward/record.url?scp=85064710489&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201801405

DO - 10.1002/mnfr.201801405

M3 - Article

AN - SCOPUS:85064710489

VL - 63

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

SN - 1613-4125

IS - 13

M1 - 1801405

ER -