Hemicellulolytic enzymes in P- and S-strains of Heterobasidion annosum

Pekka Maijala (Corresponding Author), Marjatta Raudaskoski, Liisa Viikari

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

The secretion of several hemicellulolytic enzymes and endoglucanase was studied in S- and P-group strains of a wood-rotting pathogen, Heterobasidio annosum, using enzyme activity assays and isoelectric focusing. In liquid cultures supplemented with birch sawdust or pure xylan, both strains of the fungus produced xylanases, mannanases, endoglucanases and α-galactosidases.
Low activities were detected in cultures containing only glucose as a carbon source. When S-strain cultures were provided with a cart matrix, protein secretion to the culture medium started earlier and was significantly increased compared to free cell cultures. This was not the case with the P-strain. In both strains of H. annosum the pH of the birch sawdust culture medium fell within 12 d to below 3.5, and then started to increase towards the end of the cultivation period.
The increase in pH appeared to corresprelate with the formation of hemicellulolytic and endoglucanase activitie. The isoenzyme patterns of xylanase, mannanase and endoglucanase were typical for P- and S-strains. In P-strain cultures containing xylan, a xylanase with an alkaline pl value was induced which, however, was absent when the strain was grown with the birch sawdust substrate. In the P-strain the mannanase pattern at the early stage of growth was comparable to that of t S-strain at the later stages of growth on birch sawdust medium. Later in the growth of the P-strain, this pattern was replaced by mannanases with more acid pl values.
The relative intensities of the individual endoglucanases varie between the P- and S-strains.
Original languageEnglish
Pages (from-to)743-750
JournalMicrobiology
Volume141
Issue number3
DOIs
Publication statusPublished - 1995
MoE publication typeA1 Journal article-refereed

Fingerprint

Cellulase
Betula
Enzymes
Xylans
Culture Media
Growth
Galactosidases
Enzyme Assays
Isoelectric Focusing
Isoenzymes
Fungi
Carbon
Cell Culture Techniques
Glucose
Acids
Proteins

Keywords

  • secretion
  • xylanase
  • cellulase
  • Heterobasidion annosum
  • mannanase

Cite this

Maijala, Pekka ; Raudaskoski, Marjatta ; Viikari, Liisa. / Hemicellulolytic enzymes in P- and S-strains of Heterobasidion annosum. In: Microbiology. 1995 ; Vol. 141, No. 3. pp. 743-750.
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Hemicellulolytic enzymes in P- and S-strains of Heterobasidion annosum. / Maijala, Pekka (Corresponding Author); Raudaskoski, Marjatta; Viikari, Liisa.

In: Microbiology, Vol. 141, No. 3, 1995, p. 743-750.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hemicellulolytic enzymes in P- and S-strains of Heterobasidion annosum

AU - Maijala, Pekka

AU - Raudaskoski, Marjatta

AU - Viikari, Liisa

N1 - Project code: BIO2022

PY - 1995

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N2 - The secretion of several hemicellulolytic enzymes and endoglucanase was studied in S- and P-group strains of a wood-rotting pathogen, Heterobasidio annosum, using enzyme activity assays and isoelectric focusing. In liquid cultures supplemented with birch sawdust or pure xylan, both strains of the fungus produced xylanases, mannanases, endoglucanases and α-galactosidases. Low activities were detected in cultures containing only glucose as a carbon source. When S-strain cultures were provided with a cart matrix, protein secretion to the culture medium started earlier and was significantly increased compared to free cell cultures. This was not the case with the P-strain. In both strains of H. annosum the pH of the birch sawdust culture medium fell within 12 d to below 3.5, and then started to increase towards the end of the cultivation period. The increase in pH appeared to corresprelate with the formation of hemicellulolytic and endoglucanase activitie. The isoenzyme patterns of xylanase, mannanase and endoglucanase were typical for P- and S-strains. In P-strain cultures containing xylan, a xylanase with an alkaline pl value was induced which, however, was absent when the strain was grown with the birch sawdust substrate. In the P-strain the mannanase pattern at the early stage of growth was comparable to that of t S-strain at the later stages of growth on birch sawdust medium. Later in the growth of the P-strain, this pattern was replaced by mannanases with more acid pl values. The relative intensities of the individual endoglucanases varie between the P- and S-strains.

AB - The secretion of several hemicellulolytic enzymes and endoglucanase was studied in S- and P-group strains of a wood-rotting pathogen, Heterobasidio annosum, using enzyme activity assays and isoelectric focusing. In liquid cultures supplemented with birch sawdust or pure xylan, both strains of the fungus produced xylanases, mannanases, endoglucanases and α-galactosidases. Low activities were detected in cultures containing only glucose as a carbon source. When S-strain cultures were provided with a cart matrix, protein secretion to the culture medium started earlier and was significantly increased compared to free cell cultures. This was not the case with the P-strain. In both strains of H. annosum the pH of the birch sawdust culture medium fell within 12 d to below 3.5, and then started to increase towards the end of the cultivation period. The increase in pH appeared to corresprelate with the formation of hemicellulolytic and endoglucanase activitie. The isoenzyme patterns of xylanase, mannanase and endoglucanase were typical for P- and S-strains. In P-strain cultures containing xylan, a xylanase with an alkaline pl value was induced which, however, was absent when the strain was grown with the birch sawdust substrate. In the P-strain the mannanase pattern at the early stage of growth was comparable to that of t S-strain at the later stages of growth on birch sawdust medium. Later in the growth of the P-strain, this pattern was replaced by mannanases with more acid pl values. The relative intensities of the individual endoglucanases varie between the P- and S-strains.

KW - secretion

KW - xylanase

KW - cellulase

KW - Heterobasidion annosum

KW - mannanase

U2 - 10.1099/13500872-141-3-743

DO - 10.1099/13500872-141-3-743

M3 - Article

VL - 141

SP - 743

EP - 750

JO - Microbiology

JF - Microbiology

SN - 1350-0872

IS - 3

ER -