Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

Ruixue Zhu; Tatiana Avsievich; Xinyang Su; Alexander Bykov; Alexey Popov; Igor Meglinski

doi:10.1016/j.jphotobiol.2022.112438

Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

Ruixue Zhu^*, Tatiana Avsievich, Xinyang Su, Alexander Bykov, Alexey Popov, Igor Meglinski

^*Corresponding author for this work

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Abstract

Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm² radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm² by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.

Original language	English
Article number	112438
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	230
DOIs	https://doi.org/10.1016/j.jphotobiol.2022.112438
Publication status	Published - May 2022
MoE publication type	A1 Journal article-refereed

Keywords

Aggregation
Biomodulation
Deformation
Low-level laser radiation
Optical tweezers
Red blood cells

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@article{12348ea8dea542b694422b1c6db33025,

title = "Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation",

abstract = "Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm2 radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm2 by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.",

keywords = "Aggregation, Biomodulation, Deformation, Low-level laser radiation, Optical tweezers, Red blood cells",

author = "Ruixue Zhu and Tatiana Avsievich and Xinyang Su and Alexander Bykov and Alexey Popov and Igor Meglinski",

note = "Funding Information: This work was funded by China Scholarship Council (CSC No. 201706410089 , R.Z.) and the Suomen Kulttuurirahasto (grant No. 00190188 , T.A.). The authors also acknowledge the contribution of Russian Science Foundation (project: 19-72-30012 ) and support from the Academy of Finland (project 325097 ). I.M. acknowledges the support from the Leverhulme Trust and the Royal Society (Ref. no.: APX111232 APEX Awards 2021). The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021). Publisher Copyright: {\textcopyright} 2022 The Authors",

year = "2022",

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doi = "10.1016/j.jphotobiol.2022.112438",

language = "English",

volume = "230",

journal = "Journal of Photochemistry and Photobiology B: Biology",

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T1 - Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

AU - Zhu, Ruixue

AU - Avsievich, Tatiana

AU - Su, Xinyang

AU - Bykov, Alexander

AU - Popov, Alexey

AU - Meglinski, Igor

N1 - Funding Information: This work was funded by China Scholarship Council (CSC No. 201706410089 , R.Z.) and the Suomen Kulttuurirahasto (grant No. 00190188 , T.A.). The authors also acknowledge the contribution of Russian Science Foundation (project: 19-72-30012 ) and support from the Academy of Finland (project 325097 ). I.M. acknowledges the support from the Leverhulme Trust and the Royal Society (Ref. no.: APX111232 APEX Awards 2021). The research was carried out with the support of a grant under the Decree of the Government of the Russian Federation No. 220 of 09 April 2010 (Agreement No. 075-15-2021-615 of 04 June 2021). Publisher Copyright: © 2022 The Authors

PY - 2022/5

Y1 - 2022/5

N2 - Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm2 radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm2 by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.

AB - Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm2 radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm2 by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.

KW - Aggregation

KW - Biomodulation

KW - Deformation

KW - Low-level laser radiation

KW - Optical tweezers

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AN - SCOPUS:85127773790

SN - 1011-1344

VL - 230

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

M1 - 112438

ER -

Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

Abstract

Keywords

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