Hemorheological alterations of red blood cells induced by 450-nm and 520-nm laser radiation

Ruixue Zhu, Tatiana Avsievich, Xinyang Su, Alexander Bykov, Alexey Popov, Igor Meglinski

Research output: Contribution to journalArticleScientificpeer-review

5 Citations (Scopus)


Proper rheological properties of red blood cells (RBC) including flexibility and aggregability are essential for healthy blood microcirculation. Excessive RBC aggregation has been observed to be associated with many pathological conditions and is crucial in acute circulatory problems. Low-level laser radiation (LLLR) has been found to have positive effects on the rheology of human blood, however, the detailed mechanisms of blood photobiomodulation remains unclear. In this study, utilizing the single-cell technique optical tweezers (OT) and traditional light microscopy, the influence of photobiomodulation of human RBC was examined under different conditions of laser irradiation. The results revealed that high radiant exposure (over 170.5 J/cm2 radiant fluence) caused enhanced RBC aggregation and cell shape transformation while the aggregation force between single RBC remained unchanged. LLLR with radiant fluence below 9.5 J/cm2 by 450 nm wavelength improved the RBC deformability, weakened the strength of cell-cell interaction in the RBC disaggregation process, and showed rejuvenating effects on RBC suspended in a harsh cell environment.

Original languageEnglish
Article number112438
JournalJournal of Photochemistry and Photobiology B: Biology
Publication statusPublished - May 2022
MoE publication typeA1 Journal article-refereed


  • Aggregation
  • Biomodulation
  • Deformation
  • Low-level laser radiation
  • Optical tweezers
  • Red blood cells


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