Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver

Anna Kotronen (Corresponding Author), Tuulikki Seppänen-Laakso, Jukka Westerbacka, Tuula Kiviluoto, Johanna T. Arola, Anna-Liisa Ruskeepää, Matej Orešič, Hannele Yki-Järvinen

Research output: Contribution to journalArticleScientificpeer-review

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Abstract

OBJECTIVE—To determine whether 1) hepatic ceramide and diacylglycerol concentrations, 2) SCD1 activity, and 3) hepatic lipogenic index are increased in the human nonalcoholic fatty liver.

RESEARCH DESIGN AND METHODS—We studied 16 subjects with (n = 8) and without (n = 8) histologically determined nonalcoholic fatty liver (NAFL+ and NAFL) matched for age, sex, and BMI. Hepatic concentrations of lipids and fatty acids were quantitated using ultra-performance liquid chromatography coupled to mass spectrometry and gas chromatography.

RESULTS—The absolute (nmol/mg) hepatic concentrations of diacylglycerols but not ceramides were increased in the NAFL+ group compared with the NAFL group. The livers of the NAFL+ group contained proportionally less long-chain polyunsaturated fatty acids as compared with the NAFL group. Liver fat percent was positively related to hepatic stearoyl-CoA desaturase 1 (SCD1) activity index (r = 0.70, P = 0.003) and the hepatic lipogenic index (r = 0.54, P = 0.030). Hepatic SCD1 activity index was positively related to the concentrations of diacylglycerols (r = 0.71, P = 0.002) but not ceramides (r = 0.07, NS).

CONCLUSIONS—We conclude that diacylglycerols but not ceramides are increased in NAFL. The human fatty liver is also characterized by depletion of long polyunsaturated fatty acids in the liver and increases in hepatic SCD1 and lipogenic activities.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalDiabetes
Volume58
Issue number1
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

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Stearoyl-CoA Desaturase
Ceramides
Diglycerides
Liver
Unsaturated Fatty Acids
Non-alcoholic Fatty Liver Disease
Fatty Liver
Liquid Chromatography
Gas Chromatography-Mass Spectrometry

Keywords

  • Non-alcoholic fatty liver disease
  • diacylglycerols
  • ceramides
  • SCD1
  • lipogenesis
  • long polyunsaturated fatty acids

Cite this

Kotronen, Anna ; Seppänen-Laakso, Tuulikki ; Westerbacka, Jukka ; Kiviluoto, Tuula ; Arola, Johanna T. ; Ruskeepää, Anna-Liisa ; Orešič, Matej ; Yki-Järvinen, Hannele. / Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver. In: Diabetes. 2009 ; Vol. 58, No. 1. pp. 203-208.
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title = "Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver",
abstract = "OBJECTIVE—To determine whether 1) hepatic ceramide and diacylglycerol concentrations, 2) SCD1 activity, and 3) hepatic lipogenic index are increased in the human nonalcoholic fatty liver.RESEARCH DESIGN AND METHODS—We studied 16 subjects with (n = 8) and without (n = 8) histologically determined nonalcoholic fatty liver (NAFL+ and NAFL−) matched for age, sex, and BMI. Hepatic concentrations of lipids and fatty acids were quantitated using ultra-performance liquid chromatography coupled to mass spectrometry and gas chromatography.RESULTS—The absolute (nmol/mg) hepatic concentrations of diacylglycerols but not ceramides were increased in the NAFL+ group compared with the NAFL− group. The livers of the NAFL+ group contained proportionally less long-chain polyunsaturated fatty acids as compared with the NAFL− group. Liver fat percent was positively related to hepatic stearoyl-CoA desaturase 1 (SCD1) activity index (r = 0.70, P = 0.003) and the hepatic lipogenic index (r = 0.54, P = 0.030). Hepatic SCD1 activity index was positively related to the concentrations of diacylglycerols (r = 0.71, P = 0.002) but not ceramides (r = 0.07, NS).CONCLUSIONS—We conclude that diacylglycerols but not ceramides are increased in NAFL. The human fatty liver is also characterized by depletion of long polyunsaturated fatty acids in the liver and increases in hepatic SCD1 and lipogenic activities.",
keywords = "Non-alcoholic fatty liver disease, diacylglycerols, ceramides, SCD1, lipogenesis, long polyunsaturated fatty acids",
author = "Anna Kotronen and Tuulikki Sepp{\"a}nen-Laakso and Jukka Westerbacka and Tuula Kiviluoto and Arola, {Johanna T.} and Anna-Liisa Ruskeep{\"a}{\"a} and Matej Orešič and Hannele Yki-J{\"a}rvinen",
year = "2009",
doi = "10.2337/db08-1074",
language = "English",
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pages = "203--208",
journal = "Diabetes",
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Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver. / Kotronen, Anna (Corresponding Author); Seppänen-Laakso, Tuulikki; Westerbacka, Jukka; Kiviluoto, Tuula; Arola, Johanna T.; Ruskeepää, Anna-Liisa; Orešič, Matej; Yki-Järvinen, Hannele.

In: Diabetes, Vol. 58, No. 1, 2009, p. 203-208.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hepatic stearoyl-CoA desaturase (SCD)-1 activity and diacylglycerol but not ceramide concentrations are increased in the nonalcoholic human fatty liver

AU - Kotronen, Anna

AU - Seppänen-Laakso, Tuulikki

AU - Westerbacka, Jukka

AU - Kiviluoto, Tuula

AU - Arola, Johanna T.

AU - Ruskeepää, Anna-Liisa

AU - Orešič, Matej

AU - Yki-Järvinen, Hannele

PY - 2009

Y1 - 2009

N2 - OBJECTIVE—To determine whether 1) hepatic ceramide and diacylglycerol concentrations, 2) SCD1 activity, and 3) hepatic lipogenic index are increased in the human nonalcoholic fatty liver.RESEARCH DESIGN AND METHODS—We studied 16 subjects with (n = 8) and without (n = 8) histologically determined nonalcoholic fatty liver (NAFL+ and NAFL−) matched for age, sex, and BMI. Hepatic concentrations of lipids and fatty acids were quantitated using ultra-performance liquid chromatography coupled to mass spectrometry and gas chromatography.RESULTS—The absolute (nmol/mg) hepatic concentrations of diacylglycerols but not ceramides were increased in the NAFL+ group compared with the NAFL− group. The livers of the NAFL+ group contained proportionally less long-chain polyunsaturated fatty acids as compared with the NAFL− group. Liver fat percent was positively related to hepatic stearoyl-CoA desaturase 1 (SCD1) activity index (r = 0.70, P = 0.003) and the hepatic lipogenic index (r = 0.54, P = 0.030). Hepatic SCD1 activity index was positively related to the concentrations of diacylglycerols (r = 0.71, P = 0.002) but not ceramides (r = 0.07, NS).CONCLUSIONS—We conclude that diacylglycerols but not ceramides are increased in NAFL. The human fatty liver is also characterized by depletion of long polyunsaturated fatty acids in the liver and increases in hepatic SCD1 and lipogenic activities.

AB - OBJECTIVE—To determine whether 1) hepatic ceramide and diacylglycerol concentrations, 2) SCD1 activity, and 3) hepatic lipogenic index are increased in the human nonalcoholic fatty liver.RESEARCH DESIGN AND METHODS—We studied 16 subjects with (n = 8) and without (n = 8) histologically determined nonalcoholic fatty liver (NAFL+ and NAFL−) matched for age, sex, and BMI. Hepatic concentrations of lipids and fatty acids were quantitated using ultra-performance liquid chromatography coupled to mass spectrometry and gas chromatography.RESULTS—The absolute (nmol/mg) hepatic concentrations of diacylglycerols but not ceramides were increased in the NAFL+ group compared with the NAFL− group. The livers of the NAFL+ group contained proportionally less long-chain polyunsaturated fatty acids as compared with the NAFL− group. Liver fat percent was positively related to hepatic stearoyl-CoA desaturase 1 (SCD1) activity index (r = 0.70, P = 0.003) and the hepatic lipogenic index (r = 0.54, P = 0.030). Hepatic SCD1 activity index was positively related to the concentrations of diacylglycerols (r = 0.71, P = 0.002) but not ceramides (r = 0.07, NS).CONCLUSIONS—We conclude that diacylglycerols but not ceramides are increased in NAFL. The human fatty liver is also characterized by depletion of long polyunsaturated fatty acids in the liver and increases in hepatic SCD1 and lipogenic activities.

KW - Non-alcoholic fatty liver disease

KW - diacylglycerols

KW - ceramides

KW - SCD1

KW - lipogenesis

KW - long polyunsaturated fatty acids

U2 - 10.2337/db08-1074

DO - 10.2337/db08-1074

M3 - Article

VL - 58

SP - 203

EP - 208

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 1

ER -