Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo (Corresponding Author), Astrid E. Mars, Stijn van Loon, Enrico Orsi, Gerrit Eggink, Johan P.M. Sanders, Ruud A. Weusthuis (Corresponding Author)

Research output: Contribution to journalArticleScientificpeer-review

7 Citations (Scopus)

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

Original languageEnglish
Article number849
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - Aug 2015
MoE publication typeA1 Journal article-refereed

Fingerprint

Aconitic Acid
Escherichia coli
NSC 153174
Genes
Dicarboxylic Acids
Decarboxylation
Citric Acid Cycle
Aspergillus
Industry
Polymers
Gene Expression
itaconic acid
Enzymes

Keywords

  • Cis-aconitate decarboxylase
  • Codon harmonization
  • Codon optimization
  • Escherichia coli
  • Immunoresponsive gene 1
  • Itaconic acid
  • Metabolic engineering

Cite this

Vuoristo, K. S., Mars, A. E., van Loon, S., Orsi, E., Eggink, G., Sanders, J. P. M., & Weusthuis, R. A. (2015). Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production. Frontiers in Microbiology, 6, [849]. https://doi.org/10.3389/fmicb.2015.00849
Vuoristo, Kiira S. ; Mars, Astrid E. ; van Loon, Stijn ; Orsi, Enrico ; Eggink, Gerrit ; Sanders, Johan P.M. ; Weusthuis, Ruud A. / Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production. In: Frontiers in Microbiology. 2015 ; Vol. 6.
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Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production. / Vuoristo, Kiira S. (Corresponding Author); Mars, Astrid E.; van Loon, Stijn; Orsi, Enrico; Eggink, Gerrit; Sanders, Johan P.M.; Weusthuis, Ruud A. (Corresponding Author).

In: Frontiers in Microbiology, Vol. 6, 849, 08.2015.

Research output: Contribution to journalArticleScientificpeer-review

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