Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo; Astrid E. Mars; Stijn van Loon; Enrico Orsi; Gerrit Eggink; Johan P.M. Sanders; Ruud A. Weusthuis

doi:10.3389/fmicb.2015.00849

Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo (Corresponding Author), Astrid E. Mars, Stijn van Loon, Enrico Orsi, Gerrit Eggink, Johan P.M. Sanders, Ruud A. Weusthuis (Corresponding Author)

Not published at VTT

Wageningen University & Research (WUR)

Research output: Contribution to journal › Article › Scientific › peer-review

9 Citations (Scopus)

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

Original language	English
Article number	849
Journal	Frontiers in Microbiology
Volume	6
DOIs	https://doi.org/10.3389/fmicb.2015.00849
Publication status	Published - Aug 2015
MoE publication type	A1 Journal article-refereed

Keywords

Cis-aconitate decarboxylase
Codon harmonization
Codon optimization
Escherichia coli
Immunoresponsive gene 1
Itaconic acid
Metabolic engineering

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10.3389/fmicb.2015.00849

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title = "Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production",

abstract = "Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.",

keywords = "Cis-aconitate decarboxylase, Codon harmonization, Codon optimization, Escherichia coli, Immunoresponsive gene 1, Itaconic acid, Metabolic engineering",

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month = aug,

doi = "10.3389/fmicb.2015.00849",

language = "English",

volume = "6",

journal = "Frontiers in Microbiology",

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T1 - Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

AU - Vuoristo, Kiira S.

AU - Mars, Astrid E.

AU - van Loon, Stijn

AU - Orsi, Enrico

AU - Eggink, Gerrit

AU - Sanders, Johan P.M.

AU - Weusthuis, Ruud A.

PY - 2015/8

Y1 - 2015/8

N2 - Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

AB - Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

KW - Cis-aconitate decarboxylase

KW - Codon harmonization

KW - Codon optimization

KW - Escherichia coli

KW - Immunoresponsive gene 1

KW - Itaconic acid

KW - Metabolic engineering

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U2 - 10.3389/fmicb.2015.00849

DO - 10.3389/fmicb.2015.00849

M3 - Article

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SN - 1664-302X

VL - 6

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 849

ER -

Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

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