Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo (Corresponding Author), Astrid E. Mars, Stijn van Loon, Enrico Orsi, Gerrit Eggink, Johan P.M. Sanders, Ruud A. Weusthuis (Corresponding Author)

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7 Citations (Scopus)

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

Original languageEnglish
Article number849
JournalFrontiers in Microbiology
Volume6
DOIs
Publication statusPublished - Aug 2015
MoE publication typeA1 Journal article-refereed

Keywords

  • Cis-aconitate decarboxylase
  • Codon harmonization
  • Codon optimization
  • Escherichia coli
  • Immunoresponsive gene 1
  • Itaconic acid
  • Metabolic engineering

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    Vuoristo, K. S., Mars, A. E., van Loon, S., Orsi, E., Eggink, G., Sanders, J. P. M., & Weusthuis, R. A. (2015). Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production. Frontiers in Microbiology, 6, [849]. https://doi.org/10.3389/fmicb.2015.00849