Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo; Astrid E. Mars; Stijn van Loon; Enrico Orsi; Gerrit Eggink; Johan P.M. Sanders; Ruud A. Weusthuis

doi:10.3389/fmicb.2015.00849

Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Kiira S. Vuoristo^* (Corresponding Author), Astrid E. Mars, Stijn van Loon, Enrico Orsi, Gerrit Eggink, Johan P.M. Sanders, Ruud A. Weusthuis (Corresponding Author)

^*Corresponding author for this work

Not published at VTT

Wageningen University & Research (WUR)

Research output: Contribution to journal › Article › Scientific › peer-review

13 Citations (Scopus)

Abstract

Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

Original language	English
Article number	849
Journal	Frontiers in Microbiology
Volume	6
DOIs	https://doi.org/10.3389/fmicb.2015.00849
Publication status	Published - Aug 2015
MoE publication type	A1 Journal article-refereed

Keywords

Cis-aconitate decarboxylase
Codon harmonization
Codon optimization
Escherichia coli
Immunoresponsive gene 1
Itaconic acid
Metabolic engineering

Access to Document

10.3389/fmicb.2015.00849

Cite this

@article{b686324e26f4421395f789406bfc1c32,

title = "Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production",

abstract = "Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.",

keywords = "Cis-aconitate decarboxylase, Codon harmonization, Codon optimization, Escherichia coli, Immunoresponsive gene 1, Itaconic acid, Metabolic engineering",

author = "Vuoristo, {Kiira S.} and Mars, {Astrid E.} and {van Loon}, Stijn and Enrico Orsi and Gerrit Eggink and Sanders, {Johan P.M.} and Weusthuis, {Ruud A.}",

year = "2015",

month = aug,

doi = "10.3389/fmicb.2015.00849",

language = "English",

volume = "6",

journal = "Frontiers in Microbiology",

issn = "1664-302X",

publisher = "Frontiers Media SA",

}

TY - JOUR

T1 - Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

AU - Vuoristo, Kiira S.

AU - Mars, Astrid E.

AU - van Loon, Stijn

AU - Orsi, Enrico

AU - Eggink, Gerrit

AU - Sanders, Johan P.M.

AU - Weusthuis, Ruud A.

PY - 2015/8

Y1 - 2015/8

N2 - Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

AB - Itaconic acid, a C5-dicarboxylic acid, is a potential biobased building block for the polymer industry. It is obtained from the citric acid cycle by decarboxylation of cis-aconitic acid. This reaction is catalyzed by CadA in the native itaconic acid producer Aspergillus terreus. Recently, another enzyme encoded by the mammalian immunoresponsive gene 1 (irg1), was found to decarboxylate cis-aconitate to itaconate in vitro. We show that heterologous expression of irg1 enabled itaconate production in Escherichia coli with production titres up to 560 mg/L.

KW - Cis-aconitate decarboxylase

KW - Codon harmonization

KW - Codon optimization

KW - Escherichia coli

KW - Immunoresponsive gene 1

KW - Itaconic acid

KW - Metabolic engineering

UR - http://www.scopus.com/inward/record.url?scp=84940985581&partnerID=8YFLogxK

U2 - 10.3389/fmicb.2015.00849

DO - 10.3389/fmicb.2015.00849

M3 - Article

AN - SCOPUS:84940985581

SN - 1664-302X

VL - 6

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

M1 - 849

ER -

Heterologous expression of Mus musculus immunoresponsive gene 1 (irg1) in Escherichia coli results in itaconate production

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this