Heterologous expression of Vitreoscilla haemoglobin in barley (Hordeum vulgare)

Annika Wilhelmson (Corresponding Author), Pauli T. Kallio, Kirsi-Marja Oksman-Caldentey, Anna Maria Nuutila

Research output: Contribution to journalArticleScientificpeer-review

9 Citations (Scopus)

Abstract

The vhb gene encoding Vitreoscilla haemoglobin (VHb) was transferred to barley with the aim of studying the role of oxygen availability in germination and growth. Previous findings indicate that VHb expression improves the efficiency of energy generation during oxygen-limited growth, and germination is known to be an energy demanding growth stage during which the embryos also suffer from oxygen deficiency. When subjected to oxygen deficiency, the roots of vhb-expressing barley plants showed a smaller increase in alcohol dehydrogenase (ADH) activity than those of the control plants. This indicates that VHb plants experienced less severe oxygen deficiency than the control plants, possibly due to the ability of VHb to substitute ADH for recycling NADH and maintaining glycolysis. In contrast to previous findings, we found that constitutive vhb expression did not improve the germination rate of barley kernels in any of the conditions studied. In some cases, vhb expression even slowed down germination slightly. VHb production also appeared to restrict root formation in young seedlings. The adverse effects of VHb on germination and root growth may be related to its ability to scavenge nitric oxide (NO), an important signal molecule in both seed germination and root formation. Because NO has both cytotoxic and stimulating properties, the effect of vhb expression in plants may depend on the level and role of endogenous NO in the conditions studied. VHb production also affected the levels of endogenous barley haemoglobin, which may explain the relatively moderate effects of VHb in this study.
Original languageEnglish
Pages (from-to)1773-1783
JournalPlant Cell Reports
Volume26
Issue number10
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Vitreoscilla
Hordeum vulgare
hemoglobin
barley
germination
nitric oxide
hypoxia
alcohol dehydrogenase
oxygen
energy efficiency
glycolysis
embryo (plant)
recycling
root growth
seed germination
adverse effects

Keywords

  • Barley
  • Hordeum vulgare
  • Germination
  • Haemoglobin
  • Vitreoscilla

Cite this

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title = "Heterologous expression of Vitreoscilla haemoglobin in barley (Hordeum vulgare)",
abstract = "The vhb gene encoding Vitreoscilla haemoglobin (VHb) was transferred to barley with the aim of studying the role of oxygen availability in germination and growth. Previous findings indicate that VHb expression improves the efficiency of energy generation during oxygen-limited growth, and germination is known to be an energy demanding growth stage during which the embryos also suffer from oxygen deficiency. When subjected to oxygen deficiency, the roots of vhb-expressing barley plants showed a smaller increase in alcohol dehydrogenase (ADH) activity than those of the control plants. This indicates that VHb plants experienced less severe oxygen deficiency than the control plants, possibly due to the ability of VHb to substitute ADH for recycling NADH and maintaining glycolysis. In contrast to previous findings, we found that constitutive vhb expression did not improve the germination rate of barley kernels in any of the conditions studied. In some cases, vhb expression even slowed down germination slightly. VHb production also appeared to restrict root formation in young seedlings. The adverse effects of VHb on germination and root growth may be related to its ability to scavenge nitric oxide (NO), an important signal molecule in both seed germination and root formation. Because NO has both cytotoxic and stimulating properties, the effect of vhb expression in plants may depend on the level and role of endogenous NO in the conditions studied. VHb production also affected the levels of endogenous barley haemoglobin, which may explain the relatively moderate effects of VHb in this study.",
keywords = "Barley, Hordeum vulgare, Germination, Haemoglobin, Vitreoscilla",
author = "Annika Wilhelmson and Kallio, {Pauli T.} and Kirsi-Marja Oksman-Caldentey and Nuutila, {Anna Maria}",
year = "2007",
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Heterologous expression of Vitreoscilla haemoglobin in barley (Hordeum vulgare). / Wilhelmson, Annika (Corresponding Author); Kallio, Pauli T.; Oksman-Caldentey, Kirsi-Marja; Nuutila, Anna Maria.

In: Plant Cell Reports, Vol. 26, No. 10, 2007, p. 1773-1783.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Heterologous expression of Vitreoscilla haemoglobin in barley (Hordeum vulgare)

AU - Wilhelmson, Annika

AU - Kallio, Pauli T.

AU - Oksman-Caldentey, Kirsi-Marja

AU - Nuutila, Anna Maria

PY - 2007

Y1 - 2007

N2 - The vhb gene encoding Vitreoscilla haemoglobin (VHb) was transferred to barley with the aim of studying the role of oxygen availability in germination and growth. Previous findings indicate that VHb expression improves the efficiency of energy generation during oxygen-limited growth, and germination is known to be an energy demanding growth stage during which the embryos also suffer from oxygen deficiency. When subjected to oxygen deficiency, the roots of vhb-expressing barley plants showed a smaller increase in alcohol dehydrogenase (ADH) activity than those of the control plants. This indicates that VHb plants experienced less severe oxygen deficiency than the control plants, possibly due to the ability of VHb to substitute ADH for recycling NADH and maintaining glycolysis. In contrast to previous findings, we found that constitutive vhb expression did not improve the germination rate of barley kernels in any of the conditions studied. In some cases, vhb expression even slowed down germination slightly. VHb production also appeared to restrict root formation in young seedlings. The adverse effects of VHb on germination and root growth may be related to its ability to scavenge nitric oxide (NO), an important signal molecule in both seed germination and root formation. Because NO has both cytotoxic and stimulating properties, the effect of vhb expression in plants may depend on the level and role of endogenous NO in the conditions studied. VHb production also affected the levels of endogenous barley haemoglobin, which may explain the relatively moderate effects of VHb in this study.

AB - The vhb gene encoding Vitreoscilla haemoglobin (VHb) was transferred to barley with the aim of studying the role of oxygen availability in germination and growth. Previous findings indicate that VHb expression improves the efficiency of energy generation during oxygen-limited growth, and germination is known to be an energy demanding growth stage during which the embryos also suffer from oxygen deficiency. When subjected to oxygen deficiency, the roots of vhb-expressing barley plants showed a smaller increase in alcohol dehydrogenase (ADH) activity than those of the control plants. This indicates that VHb plants experienced less severe oxygen deficiency than the control plants, possibly due to the ability of VHb to substitute ADH for recycling NADH and maintaining glycolysis. In contrast to previous findings, we found that constitutive vhb expression did not improve the germination rate of barley kernels in any of the conditions studied. In some cases, vhb expression even slowed down germination slightly. VHb production also appeared to restrict root formation in young seedlings. The adverse effects of VHb on germination and root growth may be related to its ability to scavenge nitric oxide (NO), an important signal molecule in both seed germination and root formation. Because NO has both cytotoxic and stimulating properties, the effect of vhb expression in plants may depend on the level and role of endogenous NO in the conditions studied. VHb production also affected the levels of endogenous barley haemoglobin, which may explain the relatively moderate effects of VHb in this study.

KW - Barley

KW - Hordeum vulgare

KW - Germination

KW - Haemoglobin

KW - Vitreoscilla

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DO - 10.1007/s00299-007-0393-9

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JO - Plant Cell Reports

JF - Plant Cell Reports

SN - 0721-7714

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ER -