Abstract
We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.
| Original language | English |
|---|---|
| Pages (from-to) | 987 - 990 |
| Number of pages | 4 |
| Journal | Bio/Technology |
| Volume | 9 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - 1991 |
| MoE publication type | A1 Journal article-refereed |
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Heterologous production of a ligninolytic enzyme : Expression of the Phlebia radiata laccase gene in Trichoderma reesei. / Saloheimo, Markku (Corresponding Author); Niku-Paavola, Marja-Leena.
In: Bio/Technology, Vol. 9, No. 10, 1991, p. 987 - 990.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Heterologous production of a ligninolytic enzyme
T2 - Expression of the Phlebia radiata laccase gene in Trichoderma reesei
AU - Saloheimo, Markku
AU - Niku-Paavola, Marja-Leena
N1 - Project code: BIO1022
PY - 1991
Y1 - 1991
N2 - We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.
AB - We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.
U2 - 10.1038/nbt1091-987
DO - 10.1038/nbt1091-987
M3 - Article
VL - 9
SP - 987
EP - 990
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 10
ER -