Heterologous production of a ligninolytic enzyme: Expression of the Phlebia radiata laccase gene in Trichoderma reesei

Markku Saloheimo*, Marja-Leena Niku-Paavola

*Corresponding author for this work

    Research output: Contribution to journalArticleScientificpeer-review

    78 Citations (Scopus)

    Abstract

    We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.

    Original languageEnglish
    Pages (from-to)987-990
    JournalBio/Technology
    Volume9
    Issue number10
    DOIs
    Publication statusPublished - 1991
    MoE publication typeA1 Journal article-refereed

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