Heterologous production of a ligninolytic enzyme: Expression of the Phlebia radiata laccase gene in Trichoderma reesei

Markku Saloheimo (Corresponding Author), Marja-Leena Niku-Paavola

Research output: Contribution to journalArticleScientificpeer-review

73 Citations (Scopus)

Abstract

We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.

Original languageEnglish
Pages (from-to)987 - 990
Number of pages4
JournalBio/Technology
Volume9
Issue number10
DOIs
Publication statusPublished - 1991
MoE publication typeA1 Journal article-refereed

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Laccase
Trichoderma
Enzymes
Genes
Fungi
Gene encoding
Lignin
Fermentation
Industrial applications
Cellulase
Molecular weight
Complementary DNA
Molecular Weight

Cite this

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title = "Heterologous production of a ligninolytic enzyme: Expression of the Phlebia radiata laccase gene in Trichoderma reesei",
abstract = "We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.",
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Heterologous production of a ligninolytic enzyme : Expression of the Phlebia radiata laccase gene in Trichoderma reesei. / Saloheimo, Markku (Corresponding Author); Niku-Paavola, Marja-Leena.

In: Bio/Technology, Vol. 9, No. 10, 1991, p. 987 - 990.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Heterologous production of a ligninolytic enzyme

T2 - Expression of the Phlebia radiata laccase gene in Trichoderma reesei

AU - Saloheimo, Markku

AU - Niku-Paavola, Marja-Leena

N1 - Project code: BIO1022

PY - 1991

Y1 - 1991

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AB - We have expressed a gene encoding the ligninolytic laccase enzyme of the white–rot fungus Phlebia radiata in the soft rot fungus Trichoderma reesei under the promoter of the major cellulase gene (cbh1). Constructions from the laccase cDNA or the chromosomal gene were equivalent in expression, yielding 20 mg/l of secreted active laccase in small–scale fermentations. The recombinant enzyme has a similar molecular weight, antigenic properties and specific activity as the Phlebia laccase and is analogous to the Phlebia enzyme in removing monomeric lignin–related compounds from residual lignin of the pulping process, thus demonstrating its potential for industrial applications.

U2 - 10.1038/nbt1091-987

DO - 10.1038/nbt1091-987

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JF - Nature Biotechnology

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