Heterotrophic microorganisms in air and biofilm samples from Roman catacombs, with special emphasis on actinobacteria and fungi

Maria Saarela (Corresponding Author), Hanna-Leena Alakomi, Maija-Liisa Suihko, Liisa Maunuksela, Laura Raaska, Tiina Mattila-Sandholm

Research output: Contribution to journalArticleScientificpeer-review

74 Citations (Scopus)

Abstract

The aim of the study was to characterize the heterotrophic microbiota in surface samples and in the air of the Roman catacombs of St. Callistus and St. Domitilla. The microbiotas inhabiting different environments and substrates, including plaster, marble and tufa in illuminated and dark sites, were studied. Microbial groups examined were aerobic microorganisms, anaerobic and facultatively anaerobic bacteria, proteolytic microorganisms, actinobacteria, yeasts and moulds. Filamentous actinobacteria, Streptomyces spp., were dominant in the biofilm samples. 16S rDNA sequence similarity analysis indicated that many of the isolates were novel species. Gram-negative bacteria were a minority among the isolated bacteria: few slime forming bacteria or types most closely related to them, like Pseudomonas spp., Stenotrophomonas nitritireducens, Sinorhizobium morelense and Bosea thiooxidans, were isolated. Fungi, mainly white Lecanicillium psalliotae, Torrubiella spp., Beauveria alba and L. aranearum, were isolated from both air and biofilm samples.
Original languageEnglish
Pages (from-to)27 - 37
Number of pages11
JournalInternational Biodeterioration and Biodegradation
Volume54
Issue number1
DOIs
Publication statusPublished - 2004
MoE publication typeA1 Journal article-refereed

Fingerprint

Actinobacteria
Microbiota
Biofilms
Fungi
Microorganisms
biofilm
Bacteria
Sinorhizobium
Stenotrophomonas
microorganism
Myxococcales
Air
Beauveria
fungus
bacterium
Anaerobic Bacteria
Calcium Carbonate
Streptomyces
air
Ribosomal DNA

Keywords

  • Catacomb
  • Mould
  • Actinobacterium
  • Air
  • Biofilm
  • Heterotroph

Cite this

Saarela, Maria ; Alakomi, Hanna-Leena ; Suihko, Maija-Liisa ; Maunuksela, Liisa ; Raaska, Laura ; Mattila-Sandholm, Tiina. / Heterotrophic microorganisms in air and biofilm samples from Roman catacombs, with special emphasis on actinobacteria and fungi. In: International Biodeterioration and Biodegradation. 2004 ; Vol. 54, No. 1. pp. 27 - 37.
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Heterotrophic microorganisms in air and biofilm samples from Roman catacombs, with special emphasis on actinobacteria and fungi. / Saarela, Maria (Corresponding Author); Alakomi, Hanna-Leena; Suihko, Maija-Liisa; Maunuksela, Liisa; Raaska, Laura; Mattila-Sandholm, Tiina.

In: International Biodeterioration and Biodegradation, Vol. 54, No. 1, 2004, p. 27 - 37.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Saarela, Maria

AU - Alakomi, Hanna-Leena

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