High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice

Kaisa Airaksinen (Corresponding Author), Jenna Jokkala, Ilmari Ahonen, Seppo Auriola, Marjukka Kolehmainen, Kati Hanhineva, Kirsti Tiihonen

Research output: Contribution to journalArticleScientificpeer-review

3 Citations (Scopus)

Abstract

Scope: High-fat diets are a likely cause of low-grade inflammation and obesity-related pathologies. This study measures the effects of a high-fat diet, in combination with two dietary supplements—betaine and polydextrose—on metabolism and inflammation in the adipose tissue of diet-induced obese mice. Methods and Results: Forty male C57BL/6J mice are fed a high-fat diet for 8 weeks and compared with low-fat-diet-fed control animals (n = 10). For the last 4 weeks, the high-fat-diet-fed animals are supplemented with 1% betaine, 3.33% polydextrose, their combination, or plain water. Fat depots from subcutaneous and visceral adipose tissue are analyzed for inflammatory markers and nontargeted metabolomics by quantitative PCR and LC–QTOF-MS. The high-fat diet significantly increases adipose tissue inflammation in both fat depots. By metabolic profiling, clear differences are noted between low-fat-diet and high-fat-diet groups with regard to the levels of several metabolite species—primarily carnitines, lipids, and amino acids. Dietary betaine mitigates the high-fat-diet-induced IL-6 expression and significantly increases betaine and butyrobetaine levels in adipose tissue. Conclusions: The high-fat diet induces patent changes in carnitine and lipid metabolism in adipose tissue. Betaine supplementation elevates the levels of betaine and its derivatives and certain carnitine species, as reported in muscle and liver, and moderately reduces inflammation.
Original languageEnglish
Article number1800455
JournalMolecular Nutrition and Food Research
Volume62
Issue number23
Early online date5 Oct 2018
DOIs
Publication statusPublished - 1 Dec 2018
MoE publication typeNot Eligible

Fingerprint

polydextrose
Betaine
betaine
High Fat Diet
high fat diet
Inbred C57BL Mouse
adipose tissue
Adipose Tissue
inflammation
Inflammation
metabolism
mice
Carnitine
carnitine
Fat-Restricted Diet
low fat diet
metabolomics
lipids
Fats
Obese Mice

Keywords

  • carnitine
  • inflammatory
  • lipids
  • metabolomics
  • obesity

Cite this

Airaksinen, K., Jokkala, J., Ahonen, I., Auriola, S., Kolehmainen, M., Hanhineva, K., & Tiihonen, K. (2018). High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice. Molecular Nutrition and Food Research, 62(23), [1800455]. https://doi.org/10.1002/mnfr.201800455
Airaksinen, Kaisa ; Jokkala, Jenna ; Ahonen, Ilmari ; Auriola, Seppo ; Kolehmainen, Marjukka ; Hanhineva, Kati ; Tiihonen, Kirsti. / High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice. In: Molecular Nutrition and Food Research. 2018 ; Vol. 62, No. 23.
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abstract = "Scope: High-fat diets are a likely cause of low-grade inflammation and obesity-related pathologies. This study measures the effects of a high-fat diet, in combination with two dietary supplements—betaine and polydextrose—on metabolism and inflammation in the adipose tissue of diet-induced obese mice. Methods and Results: Forty male C57BL/6J mice are fed a high-fat diet for 8 weeks and compared with low-fat-diet-fed control animals (n = 10). For the last 4 weeks, the high-fat-diet-fed animals are supplemented with 1{\%} betaine, 3.33{\%} polydextrose, their combination, or plain water. Fat depots from subcutaneous and visceral adipose tissue are analyzed for inflammatory markers and nontargeted metabolomics by quantitative PCR and LC–QTOF-MS. The high-fat diet significantly increases adipose tissue inflammation in both fat depots. By metabolic profiling, clear differences are noted between low-fat-diet and high-fat-diet groups with regard to the levels of several metabolite species—primarily carnitines, lipids, and amino acids. Dietary betaine mitigates the high-fat-diet-induced IL-6 expression and significantly increases betaine and butyrobetaine levels in adipose tissue. Conclusions: The high-fat diet induces patent changes in carnitine and lipid metabolism in adipose tissue. Betaine supplementation elevates the levels of betaine and its derivatives and certain carnitine species, as reported in muscle and liver, and moderately reduces inflammation.",
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Airaksinen, K, Jokkala, J, Ahonen, I, Auriola, S, Kolehmainen, M, Hanhineva, K & Tiihonen, K 2018, 'High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice', Molecular Nutrition and Food Research, vol. 62, no. 23, 1800455. https://doi.org/10.1002/mnfr.201800455

High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice. / Airaksinen, Kaisa (Corresponding Author); Jokkala, Jenna; Ahonen, Ilmari; Auriola, Seppo; Kolehmainen, Marjukka; Hanhineva, Kati; Tiihonen, Kirsti.

In: Molecular Nutrition and Food Research, Vol. 62, No. 23, 1800455, 01.12.2018.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - High-Fat Diet, Betaine, and Polydextrose Induce Changes in Adipose Tissue Inflammation and Metabolism in C57BL/6J Mice

AU - Airaksinen, Kaisa

AU - Jokkala, Jenna

AU - Ahonen, Ilmari

AU - Auriola, Seppo

AU - Kolehmainen, Marjukka

AU - Hanhineva, Kati

AU - Tiihonen, Kirsti

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Scope: High-fat diets are a likely cause of low-grade inflammation and obesity-related pathologies. This study measures the effects of a high-fat diet, in combination with two dietary supplements—betaine and polydextrose—on metabolism and inflammation in the adipose tissue of diet-induced obese mice. Methods and Results: Forty male C57BL/6J mice are fed a high-fat diet for 8 weeks and compared with low-fat-diet-fed control animals (n = 10). For the last 4 weeks, the high-fat-diet-fed animals are supplemented with 1% betaine, 3.33% polydextrose, their combination, or plain water. Fat depots from subcutaneous and visceral adipose tissue are analyzed for inflammatory markers and nontargeted metabolomics by quantitative PCR and LC–QTOF-MS. The high-fat diet significantly increases adipose tissue inflammation in both fat depots. By metabolic profiling, clear differences are noted between low-fat-diet and high-fat-diet groups with regard to the levels of several metabolite species—primarily carnitines, lipids, and amino acids. Dietary betaine mitigates the high-fat-diet-induced IL-6 expression and significantly increases betaine and butyrobetaine levels in adipose tissue. Conclusions: The high-fat diet induces patent changes in carnitine and lipid metabolism in adipose tissue. Betaine supplementation elevates the levels of betaine and its derivatives and certain carnitine species, as reported in muscle and liver, and moderately reduces inflammation.

AB - Scope: High-fat diets are a likely cause of low-grade inflammation and obesity-related pathologies. This study measures the effects of a high-fat diet, in combination with two dietary supplements—betaine and polydextrose—on metabolism and inflammation in the adipose tissue of diet-induced obese mice. Methods and Results: Forty male C57BL/6J mice are fed a high-fat diet for 8 weeks and compared with low-fat-diet-fed control animals (n = 10). For the last 4 weeks, the high-fat-diet-fed animals are supplemented with 1% betaine, 3.33% polydextrose, their combination, or plain water. Fat depots from subcutaneous and visceral adipose tissue are analyzed for inflammatory markers and nontargeted metabolomics by quantitative PCR and LC–QTOF-MS. The high-fat diet significantly increases adipose tissue inflammation in both fat depots. By metabolic profiling, clear differences are noted between low-fat-diet and high-fat-diet groups with regard to the levels of several metabolite species—primarily carnitines, lipids, and amino acids. Dietary betaine mitigates the high-fat-diet-induced IL-6 expression and significantly increases betaine and butyrobetaine levels in adipose tissue. Conclusions: The high-fat diet induces patent changes in carnitine and lipid metabolism in adipose tissue. Betaine supplementation elevates the levels of betaine and its derivatives and certain carnitine species, as reported in muscle and liver, and moderately reduces inflammation.

KW - carnitine

KW - inflammatory

KW - lipids

KW - metabolomics

KW - obesity

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