A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.
|Title of host publication||Optical Trapping and Optical Micromanipulation VI|
|Publisher||International Society for Optics and Photonics SPIE|
|Number of pages||8|
|Publication status||Published - Aug 2009|
|MoE publication type||B3 Non-refereed article in conference proceedings|
|Series||Proceedings of SPIE|