High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

Anders E. Wallin (Corresponding author), Heikki Ojala, Gabija Ziedaite, Linda Degerth, Dennis Bamford, Edward Haeggström

Research output: Chapter in Book/Report/Conference proceedingConference article in proceedingsScientific


Paper Abstract
A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.
Original languageEnglish
Title of host publicationOptical Trapping and Optical Micromanipulation VI
PublisherInternational Society for Optics and Photonics SPIE
Number of pages8
Publication statusPublished - Aug 2009
MoE publication typeB3 Non-refereed article in conference proceedings

Publication series

SeriesProceedings of SPIE


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