High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

Anders E. Wallin; Heikki Ojala; Gabija Ziedaite; Linda Degerth; Dennis Bamford; Edward Haeggström

doi:10.1117/12.826470

High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

Anders E. Wallin^*
, Heikki Ojala
, Gabija Ziedaite
, Linda Degerth
, Dennis Bamford
, Edward Haeggström

^*Corresponding author for this work

Not published at VTT

University of Helsinki

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

Abstract

Paper Abstract
A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.

Original language	English
Title of host publication	Optical Trapping and Optical Micromanipulation VI
Publisher	International Society for Optics and Photonics SPIE
Number of pages	8
DOIs	https://doi.org/10.1117/12.826470
Publication status	Published - Aug 2009
MoE publication type	B3 Non-refereed article in conference proceedings

Publication series

Series	Proceedings of SPIE
Volume	6644
ISSN	0277-786X

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10.1117/12.826470

Cite this

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title = "High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors",

abstract = " Paper Abstract A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.",

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High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors. / Wallin, Anders E.; Ojala, Heikki; Ziedaite, Gabija et al.
Optical Trapping and Optical Micromanipulation VI. International Society for Optics and Photonics SPIE, 2009. (Proceedings of SPIE, Vol. 6644).

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific

TY - GEN

T1 - High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

AU - Wallin, Anders E.

AU - Ojala, Heikki

AU - Ziedaite, Gabija

AU - Degerth, Linda

AU - Bamford, Dennis

AU - Haeggström, Edward

PY - 2009/8

Y1 - 2009/8

N2 - Paper Abstract A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.

AB - Paper Abstract A double-trap optical tweezers instrument was constructed and its spatial resolution measured. The instrument features real-time control that allows feedback based position- and force-clamping experiments. To study RNA-polymerization by QDE-1, an RNA-dependent RNA-polymerase, we tethered a 7250 nt single-stranded DNA molecule between two optically trapped microspheres. Preliminary constant-force extension trajectories and force-extension curves were collected.

U2 - 10.1117/12.826470

DO - 10.1117/12.826470

M3 - Conference article in proceedings

T3 - Proceedings of SPIE

BT - Optical Trapping and Optical Micromanipulation VI

PB - International Society for Optics and Photonics SPIE

ER -

High-resolution optical tweezers for investigating DNA-binding/translocating molecular motors

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