High speed microanalysis system

Heli Sirén; Lotta Amundsen; Stella Rovio; Hans Söderlund; Kristiina Takkinen; Tarja Nevanen; Ari Hokkanen; Ingmar Stuns; Kai Kolari; Santeri Tuomikoski; Sami Franssila

High speed microanalysis system

Heli Sirén, Lotta Amundsen, Stella Rovio, Hans Söderlund, Kristiina Takkinen, Tarja Nevanen, Ari Hokkanen, Ingmar Stuns, Kai Kolari, Santeri Tuomikoski, Sami Franssila

Research output: Contribution to conference › Conference article › Scientific

Abstract

A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.

Original language	Finnish
Number of pages	1
Publication status	Published - 2005
MoE publication type	Not Eligible
Event	Uudenmaan osaamiskeskuspäivät - Otaniemi, Espoo, Finland Duration: 16 Nov 2005 → 16 Nov 2005

Conference

Conference	Uudenmaan osaamiskeskuspäivät
Country/Territory	Finland
City	Otaniemi, Espoo
Period	16/11/05 → 16/11/05

Cite this

@conference{d547c34a0aa349e08644a6d41ef6ba40,

title = "High speed microanalysis system",

abstract = "A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.",

author = "Heli Sir{\'e}n and Lotta Amundsen and Stella Rovio and Hans S{\"o}derlund and Kristiina Takkinen and Tarja Nevanen and Ari Hokkanen and Ingmar Stuns and Kai Kolari and Santeri Tuomikoski and Sami Franssila",

note = "Project code: C2SU02062; Uudenmaan osaamiskeskusp{\"a}iv{\"a}t ; Conference date: 16-11-2005 Through 16-11-2005",

year = "2005",

language = "Finnish",

}

TY - CONF

T1 - High speed microanalysis system

AU - Sirén, Heli

AU - Amundsen, Lotta

AU - Rovio, Stella

AU - Söderlund, Hans

AU - Takkinen, Kristiina

AU - Nevanen, Tarja

AU - Hokkanen, Ari

AU - Stuns, Ingmar

AU - Kolari, Kai

AU - Tuomikoski, Santeri

AU - Franssila, Sami

N1 - Project code: C2SU02062

PY - 2005

Y1 - 2005

N2 - A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.

AB - A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.

M3 - Conference article

T2 - Uudenmaan osaamiskeskuspäivät

Y2 - 16 November 2005 through 16 November 2005

ER -