High speed microanalysis system

Heli Sirén, Lotta Amundsen, Stella Rovio, Hans Söderlund, Kristiina Takkinen, Tarja Nevanen, Ari Hokkanen, Ingmar Stuns, Kai Kolari, Santeri Tuomikoski, Sami Franssila

    Research output: Contribution to conferenceConference articleScientific

    Abstract

    A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.
    Original languageFinnish
    Number of pages1
    Publication statusPublished - 2005
    MoE publication typeNot Eligible
    EventUudenmaan osaamiskeskuspäivät - Otaniemi, Espoo, Finland
    Duration: 16 Nov 200516 Nov 2005

    Conference

    ConferenceUudenmaan osaamiskeskuspäivät
    CountryFinland
    CityOtaniemi, Espoo
    Period16/11/0516/11/05

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