High speed microanalysis system

Heli Sirén, Lotta Amundsen, Stella Rovio, Hans Söderlund, Kristiina Takkinen, Tarja Nevanen, Ari Hokkanen, Ingmar Stuns, Kai Kolari, Santeri Tuomikoski, Sami Franssila

Research output: Contribution to conferenceConference articleScientific

Abstract

A microanalysis system that combines analyte specific immunoextraction and subsequent capillary electrophoretic analysis was developed. The extraction chamber is filled with a solid support material coupled with antibody fragments that have specific affinity against the analyte molecules. As a model system, an anti-testosterone antibody fragment was developed and applied in the analysis of testosterone. Testosterone was labelled with fluorescein isothiocyanate (FITC) to enhance laser induced fluorescence detection. Anti-testosterone fragments were covalently immobilized on a porous polymer support material. To ensure maximal binding capacity of the immunomatrix, the antibody binding pockets were oriented away from the support material.
Original languageFinnish
Number of pages1
Publication statusPublished - 2005
MoE publication typeNot Eligible
EventUudenmaan osaamiskeskuspäivät - Otaniemi, Espoo, Finland
Duration: 16 Nov 200516 Nov 2005

Conference

ConferenceUudenmaan osaamiskeskuspäivät
CountryFinland
CityOtaniemi, Espoo
Period16/11/0516/11/05

Cite this

Sirén, H., Amundsen, L., Rovio, S., Söderlund, H., Takkinen, K., Nevanen, T., Hokkanen, A., Stuns, I., Kolari, K., Tuomikoski, S., & Franssila, S. (2005). High speed microanalysis system. Paper presented at Uudenmaan osaamiskeskuspäivät, Otaniemi, Espoo, Finland.