High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production

Karin Öhgren, Jari Vehmaanperä, Matti Siika-Aho, Mats Galbe, Liisa Viikari, Guido Zacchi

Research output: Contribution to journalArticleScientificpeer-review

122 Citations (Scopus)

Abstract

As a consequence of heightened concern for the greenhouse effect, depleting oil reserves and skyrocketing oil prices, renewable fuels, such as bioethanol, are becoming increasingly important. Cellulosic biomass like wood, agricultural residue and herbaceous material is a huge, cheap natural resource that can be used for ethanol production. Decreasing production costs through technological development is, however, still needed to make ethanol from cellulosics economically feasible. In this study, the effect on overall ethanol yield of an enzymatic prehydrolysis prior to simultaneous saccharification and fermentation (SSF) of steam pretreated corn stover was investigated. Two enzyme mixtures were utilised. A mixture of commercially available enzymes (Novozymes A/S, Baegersvaerd, Denmark) was compared with a developmental thermo-active cellulase complex produced by Roal Oy (Rajamäki, Finland). The thermoactive preparation comprised three essential cellulases and one xylanase enzyme. The prehydrolysis was, under the conditions evaluated, found to have no or negative effect on the overall ethanol yield. Longer prehydrolysis time resulted in a larger decrease in overall ethanol yield than shorter prehydrolysis. Using the experimental thermo-active enzyme mixture was shown to give a higher glucose concentration after prehydrolysis than when the commercial enzyme mixture was used in similar experiments. The highest ethanol concentration, 33.8 g/L, was reached in the SSF with 11.5% water-insoluble substances using the developmental thermo-active cellulase complex, and 1.8 g/L compressed baker's yeast. This concentration corresponded to 80.2% overall ethanol yield based on the glucose content in the raw material. However, if the xylose present in the beer at the end of the SSF could be fermented to ethanol, another 12.6 g ethanol/L could theoretically be produced (0.51 g ethanol/g xylose).
Original languageEnglish
Pages (from-to)607-613
JournalEnzyme and Microbial Technology
Volume40
Issue number4
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fingerprint

Saccharification
Steam
Fermentation
Zea mays
Ethanol
Temperature
Enzymes
Xylose
Cellulase
Glucose
Oils
Greenhouse Effect
Greenhouse effect
Cellulases
Beer
Agricultural wastes
Bioethanol
Natural resources
Denmark
Finland

Keywords

  • Prehydrolysis
  • SSF
  • Ethanol production
  • Ethanol yield
  • Steam pretreatment
  • Thermo-active cellulase complex

Cite this

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title = "High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production",
abstract = "As a consequence of heightened concern for the greenhouse effect, depleting oil reserves and skyrocketing oil prices, renewable fuels, such as bioethanol, are becoming increasingly important. Cellulosic biomass like wood, agricultural residue and herbaceous material is a huge, cheap natural resource that can be used for ethanol production. Decreasing production costs through technological development is, however, still needed to make ethanol from cellulosics economically feasible. In this study, the effect on overall ethanol yield of an enzymatic prehydrolysis prior to simultaneous saccharification and fermentation (SSF) of steam pretreated corn stover was investigated. Two enzyme mixtures were utilised. A mixture of commercially available enzymes (Novozymes A/S, Baegersvaerd, Denmark) was compared with a developmental thermo-active cellulase complex produced by Roal Oy (Rajam{\"a}ki, Finland). The thermoactive preparation comprised three essential cellulases and one xylanase enzyme. The prehydrolysis was, under the conditions evaluated, found to have no or negative effect on the overall ethanol yield. Longer prehydrolysis time resulted in a larger decrease in overall ethanol yield than shorter prehydrolysis. Using the experimental thermo-active enzyme mixture was shown to give a higher glucose concentration after prehydrolysis than when the commercial enzyme mixture was used in similar experiments. The highest ethanol concentration, 33.8 g/L, was reached in the SSF with 11.5{\%} water-insoluble substances using the developmental thermo-active cellulase complex, and 1.8 g/L compressed baker's yeast. This concentration corresponded to 80.2{\%} overall ethanol yield based on the glucose content in the raw material. However, if the xylose present in the beer at the end of the SSF could be fermented to ethanol, another 12.6 g ethanol/L could theoretically be produced (0.51 g ethanol/g xylose).",
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author = "Karin {\"O}hgren and Jari Vehmaanper{\"a} and Matti Siika-Aho and Mats Galbe and Liisa Viikari and Guido Zacchi",
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High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production. / Öhgren, Karin; Vehmaanperä, Jari; Siika-Aho, Matti; Galbe, Mats; Viikari, Liisa; Zacchi, Guido.

In: Enzyme and Microbial Technology, Vol. 40, No. 4, 2007, p. 607-613.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - High temperature enzymatic prehydrolysis prior to simultaneous saccharification and fermentation of steam pretreated corn stover for ethanol production

AU - Öhgren, Karin

AU - Vehmaanperä, Jari

AU - Siika-Aho, Matti

AU - Galbe, Mats

AU - Viikari, Liisa

AU - Zacchi, Guido

PY - 2007

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N2 - As a consequence of heightened concern for the greenhouse effect, depleting oil reserves and skyrocketing oil prices, renewable fuels, such as bioethanol, are becoming increasingly important. Cellulosic biomass like wood, agricultural residue and herbaceous material is a huge, cheap natural resource that can be used for ethanol production. Decreasing production costs through technological development is, however, still needed to make ethanol from cellulosics economically feasible. In this study, the effect on overall ethanol yield of an enzymatic prehydrolysis prior to simultaneous saccharification and fermentation (SSF) of steam pretreated corn stover was investigated. Two enzyme mixtures were utilised. A mixture of commercially available enzymes (Novozymes A/S, Baegersvaerd, Denmark) was compared with a developmental thermo-active cellulase complex produced by Roal Oy (Rajamäki, Finland). The thermoactive preparation comprised three essential cellulases and one xylanase enzyme. The prehydrolysis was, under the conditions evaluated, found to have no or negative effect on the overall ethanol yield. Longer prehydrolysis time resulted in a larger decrease in overall ethanol yield than shorter prehydrolysis. Using the experimental thermo-active enzyme mixture was shown to give a higher glucose concentration after prehydrolysis than when the commercial enzyme mixture was used in similar experiments. The highest ethanol concentration, 33.8 g/L, was reached in the SSF with 11.5% water-insoluble substances using the developmental thermo-active cellulase complex, and 1.8 g/L compressed baker's yeast. This concentration corresponded to 80.2% overall ethanol yield based on the glucose content in the raw material. However, if the xylose present in the beer at the end of the SSF could be fermented to ethanol, another 12.6 g ethanol/L could theoretically be produced (0.51 g ethanol/g xylose).

AB - As a consequence of heightened concern for the greenhouse effect, depleting oil reserves and skyrocketing oil prices, renewable fuels, such as bioethanol, are becoming increasingly important. Cellulosic biomass like wood, agricultural residue and herbaceous material is a huge, cheap natural resource that can be used for ethanol production. Decreasing production costs through technological development is, however, still needed to make ethanol from cellulosics economically feasible. In this study, the effect on overall ethanol yield of an enzymatic prehydrolysis prior to simultaneous saccharification and fermentation (SSF) of steam pretreated corn stover was investigated. Two enzyme mixtures were utilised. A mixture of commercially available enzymes (Novozymes A/S, Baegersvaerd, Denmark) was compared with a developmental thermo-active cellulase complex produced by Roal Oy (Rajamäki, Finland). The thermoactive preparation comprised three essential cellulases and one xylanase enzyme. The prehydrolysis was, under the conditions evaluated, found to have no or negative effect on the overall ethanol yield. Longer prehydrolysis time resulted in a larger decrease in overall ethanol yield than shorter prehydrolysis. Using the experimental thermo-active enzyme mixture was shown to give a higher glucose concentration after prehydrolysis than when the commercial enzyme mixture was used in similar experiments. The highest ethanol concentration, 33.8 g/L, was reached in the SSF with 11.5% water-insoluble substances using the developmental thermo-active cellulase complex, and 1.8 g/L compressed baker's yeast. This concentration corresponded to 80.2% overall ethanol yield based on the glucose content in the raw material. However, if the xylose present in the beer at the end of the SSF could be fermented to ethanol, another 12.6 g ethanol/L could theoretically be produced (0.51 g ethanol/g xylose).

KW - Prehydrolysis

KW - SSF

KW - Ethanol production

KW - Ethanol yield

KW - Steam pretreatment

KW - Thermo-active cellulase complex

U2 - 10.1016/j.enzmictec.2006.05.014

DO - 10.1016/j.enzmictec.2006.05.014

M3 - Article

VL - 40

SP - 607

EP - 613

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -