High-volume fabrication of sylgard 184 devices for single cell analytics

Christina Liedert; Benedek Poor; Olli Heikki Huttunen; Johanna Hiitola-Keinänen; Sanna Aikio; Heli Pessa; Pinja Elomaa; Jussi Hiltunen; Päivi Saavalainen; Leena Hakalahti

High-volume fabrication of sylgard 184 devices for single cell analytics

Christina Liedert, Benedek Poor, Olli Heikki Huttunen, Johanna Hiitola-Keinänen, Sanna Aikio, Heli Pessa, Pinja Elomaa, Jussi Hiltunen, Päivi Saavalainen, Leena Hakalahti

University of Helsinki

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

Abstract

Several lab-on-chip prototypes rely on PDMS (polydimethylsiloxane) processed with laborious lithographic techniques. Upscaling the manufacturing volumes requires typically material change which increases the market entry time and costs. As a solution, a novel, high-volume fabrication method called Roll-to-Roll PDMS thermal imprinting is presented. This study demonstrates high accuracy and high volume (480 chips/h) replication of microwells for single cell RNA sequencing (Seq-Well). Sealing of these devices with semipermeable membrane followed by lysis of compartmentalized human cells is further shown.

Original language	English
Title of host publication	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Pages	1138-1139
Number of pages	2
ISBN (Electronic)	978-173341900-0
Publication status	Published - 2019
MoE publication type	A4 Article in a conference publication
Event	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019

Conference

Conference	23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019
Country/Territory	Switzerland
City	Basel
Period	27/10/19 → 31/10/19

Keywords

PDMS (polydimethylsiloxane)
Roll-to-roll imprinting
Single cell RNA sequencing

Cite this

@inproceedings{bcdbac6bac3f4c7abf3197063d056f77,

title = "High-volume fabrication of sylgard 184 devices for single cell analytics",

abstract = "Several lab-on-chip prototypes rely on PDMS (polydimethylsiloxane) processed with laborious lithographic techniques. Upscaling the manufacturing volumes requires typically material change which increases the market entry time and costs. As a solution, a novel, high-volume fabrication method called Roll-to-Roll PDMS thermal imprinting is presented. This study demonstrates high accuracy and high volume (480 chips/h) replication of microwells for single cell RNA sequencing (Seq-Well). Sealing of these devices with semipermeable membrane followed by lysis of compartmentalized human cells is further shown.",

keywords = "PDMS (polydimethylsiloxane), Roll-to-roll imprinting, Single cell RNA sequencing",

author = "Christina Liedert and Benedek Poor and Huttunen, {Olli Heikki} and Johanna Hiitola-Kein{\"a}nen and Sanna Aikio and Heli Pessa and Pinja Elomaa and Jussi Hiltunen and P{\"a}ivi Saavalainen and Leena Hakalahti",

note = "Funding Information: We thank VTT{\textquoteright}s pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018. Funding Information: We thank VTT's pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018. Publisher Copyright: {\textcopyright} 2019 CBMS-0001.; 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 ; Conference date: 27-10-2019 Through 31-10-2019",

year = "2019",

language = "English",

pages = "1138--1139",

booktitle = "23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019",

}

Liedert, C, Poor, B, Huttunen, OH , Hiitola-Keinänen, J , Aikio, S, Pessa, H, Elomaa, P, Hiltunen, J, Saavalainen, P & Hakalahti, L 2019, High-volume fabrication of sylgard 184 devices for single cell analytics. in 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. pp. 1138-1139, 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019, Basel, Switzerland, 27/10/19.

High-volume fabrication of sylgard 184 devices for single cell analytics. / Liedert, Christina; Poor, Benedek; Huttunen, Olli Heikki et al.
23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019. 2019. p. 1138-1139.

Research output: Chapter in Book/Report/Conference proceeding › Conference article in proceedings › Scientific › peer-review

TY - GEN

T1 - High-volume fabrication of sylgard 184 devices for single cell analytics

AU - Liedert, Christina

AU - Poor, Benedek

AU - Huttunen, Olli Heikki

AU - Hiitola-Keinänen, Johanna

AU - Aikio, Sanna

AU - Pessa, Heli

AU - Elomaa, Pinja

AU - Hiltunen, Jussi

AU - Saavalainen, Päivi

AU - Hakalahti, Leena

N1 - Funding Information: We thank VTT’s pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018. Funding Information: We thank VTT's pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018. Publisher Copyright: © 2019 CBMS-0001.

PY - 2019

Y1 - 2019

N2 - Several lab-on-chip prototypes rely on PDMS (polydimethylsiloxane) processed with laborious lithographic techniques. Upscaling the manufacturing volumes requires typically material change which increases the market entry time and costs. As a solution, a novel, high-volume fabrication method called Roll-to-Roll PDMS thermal imprinting is presented. This study demonstrates high accuracy and high volume (480 chips/h) replication of microwells for single cell RNA sequencing (Seq-Well). Sealing of these devices with semipermeable membrane followed by lysis of compartmentalized human cells is further shown.

AB - Several lab-on-chip prototypes rely on PDMS (polydimethylsiloxane) processed with laborious lithographic techniques. Upscaling the manufacturing volumes requires typically material change which increases the market entry time and costs. As a solution, a novel, high-volume fabrication method called Roll-to-Roll PDMS thermal imprinting is presented. This study demonstrates high accuracy and high volume (480 chips/h) replication of microwells for single cell RNA sequencing (Seq-Well). Sealing of these devices with semipermeable membrane followed by lysis of compartmentalized human cells is further shown.

KW - PDMS (polydimethylsiloxane)

KW - Roll-to-roll imprinting

KW - Single cell RNA sequencing

UR - http://www.scopus.com/inward/record.url?scp=85094965175&partnerID=8YFLogxK

M3 - Conference article in proceedings

AN - SCOPUS:85094965175

SP - 1138

EP - 1139

BT - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

T2 - 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019

Y2 - 27 October 2019 through 31 October 2019

ER -

High-volume fabrication of sylgard 184 devices for single cell analytics

Abstract

Conference

Keywords

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