Abstract
Several lab-on-chip prototypes rely on PDMS (polydimethylsiloxane) processed with laborious lithographic techniques. Upscaling the manufacturing volumes requires typically material change which increases the market entry time and costs. As a solution, a novel, high-volume fabrication method called Roll-to-Roll PDMS thermal imprinting is presented. This study demonstrates high accuracy and high volume (480 chips/h) replication of microwells for single cell RNA sequencing (Seq-Well). Sealing of these devices with semipermeable membrane followed by lysis of compartmentalized human cells is further shown.
| Original language | English |
|---|---|
| Title of host publication | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
| Pages | 1138-1139 |
| Number of pages | 2 |
| ISBN (Electronic) | 978-173341900-0 |
| Publication status | Published - 2019 |
| MoE publication type | A4 Article in a conference publication |
| Event | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 - Basel, Switzerland Duration: 27 Oct 2019 → 31 Oct 2019 |
Conference
| Conference | 23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019 |
|---|---|
| Country/Territory | Switzerland |
| City | Basel |
| Period | 27/10/19 → 31/10/19 |
Funding
We thank VTT’s pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018. We thank VTT's pilot line operator Mrs. Jenni Tomperi for her assistance. The study received financial support from Business Finland, grant 5490/31/2018.
Keywords
- PDMS (polydimethylsiloxane)
- Roll-to-roll imprinting
- Single cell RNA sequencing
