High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt

Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström (Corresponding Author), Katariina Rommi, Pia Willberg-Keyriläinen, Dilek Ercili-Cura, Ulla Holopainen-Mantila, Jaakko Hiltunen, Outi Mäkinen, Heli Nygren, Atte Mikkelson, Lauri Kuutti

Research output: Contribution to journalArticleScientificpeer-review

2 Citations (Scopus)

Abstract

The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.
Original languageEnglish
Pages (from-to)9355-9363
Number of pages9
JournalChemistrySelect
Volume2
Issue number29
DOIs
Publication statusPublished - 1 Jan 2017
MoE publication typeA1 Journal article-refereed

Fingerprint

Eutectics
Urea
Salts
Proteins
Brewing
Choline
Sustainable development
Microscopic examination
Dissolution

Keywords

  • brewer's spent grain
  • deep eutectic solvent
  • fractionation
  • ionic liquid
  • protein

Cite this

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title = "High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents",
abstract = "The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt{\%} NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79{\%} extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt{\%} NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt{\%} was demonstrated and the fractions were characterized in detail.",
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High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt : Urea Aqueous Deep Eutectic Solvents. / Wahlström, Ronny (Corresponding Author); Rommi, Katariina; Willberg-Keyriläinen, Pia; Ercili-Cura, Dilek; Holopainen-Mantila, Ulla; Hiltunen, Jaakko; Mäkinen, Outi; Nygren, Heli; Mikkelson, Atte; Kuutti, Lauri.

In: ChemistrySelect, Vol. 2, No. 29, 01.01.2017, p. 9355-9363.

Research output: Contribution to journalArticleScientificpeer-review

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AU - Holopainen-Mantila, Ulla

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AU - Nygren, Heli

AU - Mikkelson, Atte

AU - Kuutti, Lauri

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