High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström; Katariina Rommi; Pia Willberg-Keyriläinen; Dilek Ercili-Cura; Ulla Holopainen-Mantila; Jaakko Hiltunen; Outi Mäkinen; Heli Nygren; Atte Mikkelson; Lauri Kuutti

doi:10.1002/slct.201701492

High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström (Corresponding Author), Katariina Rommi, Pia Willberg-Keyriläinen, Dilek Ercili-Cura, Ulla Holopainen-Mantila, Jaakko Hiltunen, Outi Mäkinen, Heli Nygren, Atte Mikkelson, Lauri Kuutti

Research output: Contribution to journal › Article › Scientific › peer-review

5 Citations (Scopus)

Abstract

The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.

Original language	English
Pages (from-to)	9355-9363
Number of pages	9
Journal	ChemistrySelect
Volume	2
Issue number	29
DOIs	https://doi.org/10.1002/slct.201701492
Publication status	Published - 1 Jan 2017
MoE publication type	A1 Journal article-refereed

Keywords

brewer's spent grain
deep eutectic solvent
fractionation
ionic liquid
protein

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10.1002/slct.201701492

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Wahlström, R., Rommi, K., Willberg-Keyriläinen, P., Ercili-Cura, D., Holopainen-Mantila, U., Hiltunen, J., Mäkinen, O., Nygren, H., Mikkelson, A., & Kuutti, L. (2017). High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents. ChemistrySelect, 2(29), 9355-9363. https://doi.org/10.1002/slct.201701492

Wahlström, Ronny ; Rommi, Katariina ; Willberg-Keyriläinen, Pia ; Ercili-Cura, Dilek ; Holopainen-Mantila, Ulla ; Hiltunen, Jaakko ; Mäkinen, Outi ; Nygren, Heli ; Mikkelson, Atte ; Kuutti, Lauri. / High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt : Urea Aqueous Deep Eutectic Solvents. In: ChemistrySelect. 2017 ; Vol. 2, No. 29. pp. 9355-9363.

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abstract = "The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.",

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High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt : Urea Aqueous Deep Eutectic Solvents. / Wahlström, Ronny (Corresponding Author); Rommi, Katariina; Willberg-Keyriläinen, Pia; Ercili-Cura, Dilek; Holopainen-Mantila, Ulla; Hiltunen, Jaakko; Mäkinen, Outi; Nygren, Heli ; Mikkelson, Atte ; Kuutti, Lauri.

In: ChemistrySelect, Vol. 2, No. 29, 01.01.2017, p. 9355-9363.

Research output: Contribution to journal › Article › Scientific › peer-review

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