High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström (Corresponding Author), Katariina Rommi, Pia Willberg-Keyriläinen, Dilek Ercili-Cura, Ulla Holopainen-Mantila, Jaakko Hiltunen, Outi Mäkinen, Heli Nygren, Atte Mikkelson, Lauri Kuutti

    Research output: Contribution to journalArticleScientificpeer-review

    13 Citations (Scopus)

    Abstract

    The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.
    Original languageEnglish
    Pages (from-to)9355-9363
    JournalChemistrySelect
    Volume2
    Issue number29
    DOIs
    Publication statusPublished - 11 Oct 2017
    MoE publication typeA1 Journal article-refereed

    Keywords

    • brewer's spent grain
    • deep eutectic solvent
    • fractionation
    • ionic liquid
    • protein

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