TY - JOUR
T1 - High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt
T2 - Urea Aqueous Deep Eutectic Solvents
AU - Wahlström, Ronny
AU - Rommi, Katariina
AU - Willberg-Keyriläinen, Pia
AU - Ercili-Cura, Dilek
AU - Holopainen-Mantila, Ulla
AU - Hiltunen, Jaakko
AU - Mäkinen, Outi
AU - Nygren, Heli
AU - Mikkelson, Atte
AU - Kuutti, Lauri
N1 - Funding Information:
The authors gratefully acknowledge the VTT Business from Emerging Technologies (iBET) program for funding this study. Mari Leino, Ritva Heinonen, Jenni Lehtonen and Helinä Heikkinen at VTT - Technical Research Centre of Finland Ltd are thanked for skillful technical assistance. Funding from the Academy of Finland (grant number 286124) is gratefully acknowledged (Ronny Wahlström).
Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2017/10/11
Y1 - 2017/10/11
N2 - The sustainability of the global protein production is
seriously challenged, why upgrading protein from new or
under-utilized sources such as brewer's spent grain (BSG)
is desirable. In this article a new method for high-yield
protein extraction from BSG with deep eutectic solvents
(DESs) is reported. Novel carboxylate salt - urea DESs
are described and protein extraction from BSG using 6 of
these mixtures is compared. The extraction with 90 wt%
NaAcO : urea (molar ratio 1:2) was compared to choline
chloride : urea (1:2), of which the first was found to be
a more suitable protein extractant with up to 79%
extraction yields from BSG. Based on microscopy, it is
proposed that the high extraction yield from BSG with 90
wt% NaAcO : urea (1:2) was due to dissolution of proteins
insoluble by nature or denatured during the brewing
process. The production of protein concentrates with a
protein content of >50 wt% was demonstrated and the
fractions were characterized in detail.
AB - The sustainability of the global protein production is
seriously challenged, why upgrading protein from new or
under-utilized sources such as brewer's spent grain (BSG)
is desirable. In this article a new method for high-yield
protein extraction from BSG with deep eutectic solvents
(DESs) is reported. Novel carboxylate salt - urea DESs
are described and protein extraction from BSG using 6 of
these mixtures is compared. The extraction with 90 wt%
NaAcO : urea (molar ratio 1:2) was compared to choline
chloride : urea (1:2), of which the first was found to be
a more suitable protein extractant with up to 79%
extraction yields from BSG. Based on microscopy, it is
proposed that the high extraction yield from BSG with 90
wt% NaAcO : urea (1:2) was due to dissolution of proteins
insoluble by nature or denatured during the brewing
process. The production of protein concentrates with a
protein content of >50 wt% was demonstrated and the
fractions were characterized in detail.
KW - brewer's spent grain
KW - deep eutectic solvent
KW - fractionation
KW - ionic liquid
KW - protein
UR - http://www.scopus.com/inward/record.url?scp=85041961458&partnerID=8YFLogxK
U2 - 10.1002/slct.201701492
DO - 10.1002/slct.201701492
M3 - Article
VL - 2
SP - 9355
EP - 9363
JO - ChemistrySelect
JF - ChemistrySelect
SN - 2365-6549
IS - 29
ER -