Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 9355-9363 |
| Number of pages | 9 |
| Journal | ChemistrySelect |
| Volume | 2 |
| Issue number | 29 |
| DOIs | |
| Publication status | Published - 1 Jan 2017 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- brewer's spent grain
- deep eutectic solvent
- fractionation
- ionic liquid
- protein
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High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt : Urea Aqueous Deep Eutectic Solvents. / Wahlström, Ronny (Corresponding Author); Rommi, Katariina; Willberg-Keyriläinen, Pia; Ercili-Cura, Dilek; Holopainen-Mantila, Ulla; Hiltunen, Jaakko; Mäkinen, Outi; Nygren, Heli; Mikkelson, Atte; Kuutti, Lauri.
In: ChemistrySelect, Vol. 2, No. 29, 01.01.2017, p. 9355-9363.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt
T2 - Urea Aqueous Deep Eutectic Solvents
AU - Wahlström, Ronny
AU - Rommi, Katariina
AU - Willberg-Keyriläinen, Pia
AU - Ercili-Cura, Dilek
AU - Holopainen-Mantila, Ulla
AU - Hiltunen, Jaakko
AU - Mäkinen, Outi
AU - Nygren, Heli
AU - Mikkelson, Atte
AU - Kuutti, Lauri
PY - 2017/1/1
Y1 - 2017/1/1
N2 - The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.
AB - The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.
KW - brewer's spent grain
KW - deep eutectic solvent
KW - fractionation
KW - ionic liquid
KW - protein
UR - http://www.scopus.com/inward/record.url?scp=85041961458&partnerID=8YFLogxK
U2 - 10.1002/slct.201701492
DO - 10.1002/slct.201701492
M3 - Article
VL - 2
SP - 9355
EP - 9363
JO - ChemistrySelect
JF - ChemistrySelect
SN - 2365-6549
IS - 29
ER -