High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström; Katariina Rommi; Pia Willberg-Keyriläinen; Dilek Ercili-Cura; Ulla Holopainen-Mantila; Jaakko Hiltunen; Outi Mäkinen; Heli Nygren; Atte Mikkelson; Lauri Kuutti

doi:10.1002/slct.201701492

High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Ronny Wahlström^*
, Katariina Rommi
, Pia Willberg-Keyriläinen
, Dilek Ercili-Cura
, Ulla Holopainen-Mantila
, Jaakko Hiltunen
, Outi Mäkinen
, Heli Nygren
, Atte Mikkelson
, Lauri Kuutti

^*Corresponding author for this work

VTT (former employee or external)

Research output: Contribution to journal › Article › Scientific › peer-review

84 Citations (Scopus)

Abstract

The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.

Original language	English
Pages (from-to)	9355-9363
Journal	ChemistrySelect
Volume	2
Issue number	29
DOIs	https://doi.org/10.1002/slct.201701492
Publication status	Published - 11 Oct 2017
MoE publication type	A1 Journal article-refereed

Funding

The authors gratefully acknowledge the VTT Business from Emerging Technologies (iBET) program for funding this study. Funding from the Academy of Finland (grant number 286124) is gratefully acknowledged (Ronny Wahlström).

Keywords

brewer's spent grain
deep eutectic solvent
fractionation
ionic liquid
protein

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10.1002/slct.201701492

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title = "High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents",

abstract = "The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt\% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79\% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt\% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt\% was demonstrated and the fractions were characterized in detail.",

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AU - Willberg-Keyriläinen, Pia

AU - Ercili-Cura, Dilek

AU - Holopainen-Mantila, Ulla

AU - Hiltunen, Jaakko

AU - Mäkinen, Outi

AU - Nygren, Heli

AU - Mikkelson, Atte

AU - Kuutti, Lauri

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AB - The sustainability of the global protein production is seriously challenged, why upgrading protein from new or under-utilized sources such as brewer's spent grain (BSG) is desirable. In this article a new method for high-yield protein extraction from BSG with deep eutectic solvents (DESs) is reported. Novel carboxylate salt - urea DESs are described and protein extraction from BSG using 6 of these mixtures is compared. The extraction with 90 wt% NaAcO : urea (molar ratio 1:2) was compared to choline chloride : urea (1:2), of which the first was found to be a more suitable protein extractant with up to 79% extraction yields from BSG. Based on microscopy, it is proposed that the high extraction yield from BSG with 90 wt% NaAcO : urea (1:2) was due to dissolution of proteins insoluble by nature or denatured during the brewing process. The production of protein concentrates with a protein content of >50 wt% was demonstrated and the fractions were characterized in detail.

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KW - fractionation

KW - ionic liquid

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High Yield Protein Extraction from Brewer's Spent Grainwith Novel Carboxylate Salt: Urea Aqueous Deep Eutectic Solvents

Abstract

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Keywords

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