Highly specific layers and non-label detection methods

Inger Vikholm-Lundin; Kirsi Tappura

Highly specific layers and non-label detection methods

Inger Vikholm-Lundin, Kirsi Tappura

Research output: Contribution to conference › Conference article › Scientific › peer-review

Abstract

We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

Original language	English
Publication status	Published - 2009
MoE publication type	Not Eligible
Event	International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009 - Dublin, Ireland Duration: 26 Jan 2009 → 27 Jan 2009

Conference

Conference	International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009
Abbreviated title	ICTBSB
Country/Territory	Ireland
City	Dublin
Period	26/01/09 → 27/01/09

Cite this

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title = "Highly specific layers and non-label detection methods",

abstract = "We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.",

author = "Inger Vikholm-Lundin and Kirsi Tappura",

note = "ext. abstract; International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, ICTBSB ; Conference date: 26-01-2009 Through 27-01-2009",

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language = "English",

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TY - CONF

T1 - Highly specific layers and non-label detection methods

AU - Vikholm-Lundin, Inger

AU - Tappura, Kirsi

N1 - ext. abstract

PY - 2009

Y1 - 2009

N2 - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

AB - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

M3 - Conference article

T2 - International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009

Y2 - 26 January 2009 through 27 January 2009

ER -

Highly specific layers and non-label detection methods

Abstract

Conference

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