Highly specific layers and non-label detection methods

Inger Vikholm-Lundin, Kirsi Tappura

Research output: Contribution to conferenceConference articleScientificpeer-review

Abstract

We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.
Original languageEnglish
Publication statusPublished - 2009
MoE publication typeNot Eligible
EventInternational Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009 - Dublin, Ireland
Duration: 26 Jan 200927 Jan 2009

Conference

ConferenceInternational Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009
Abbreviated titleICTBSB
CountryIreland
CityDublin
Period26/01/0927/01/09

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Artificial Receptors
Antibodies
Sensors
Surface plasmon resonance
Antigens

Cite this

Vikholm-Lundin, I., & Tappura, K. (2009). Highly specific layers and non-label detection methods. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.
Vikholm-Lundin, Inger ; Tappura, Kirsi. / Highly specific layers and non-label detection methods. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.
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author = "Inger Vikholm-Lundin and Kirsi Tappura",
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Vikholm-Lundin, I & Tappura, K 2009, 'Highly specific layers and non-label detection methods' Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland, 26/01/09 - 27/01/09, .

Highly specific layers and non-label detection methods. / Vikholm-Lundin, Inger; Tappura, Kirsi.

2009. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.

Research output: Contribution to conferenceConference articleScientificpeer-review

TY - CONF

T1 - Highly specific layers and non-label detection methods

AU - Vikholm-Lundin, Inger

AU - Tappura, Kirsi

N1 - ext. abstract

PY - 2009

Y1 - 2009

N2 - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

AB - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

M3 - Conference article

ER -

Vikholm-Lundin I, Tappura K. Highly specific layers and non-label detection methods. 2009. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.