Highly specific layers and non-label detection methods

Inger Vikholm-Lundin, Kirsi Tappura

    Research output: Contribution to conferenceConference articleScientificpeer-review

    Abstract

    We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.
    Original languageEnglish
    Publication statusPublished - 2009
    MoE publication typeNot Eligible
    EventInternational Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009 - Dublin, Ireland
    Duration: 26 Jan 200927 Jan 2009

    Conference

    ConferenceInternational Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009
    Abbreviated titleICTBSB
    CountryIreland
    CityDublin
    Period26/01/0927/01/09

    Fingerprint

    Artificial Receptors
    Antibodies
    Sensors
    Surface plasmon resonance
    Antigens

    Cite this

    Vikholm-Lundin, I., & Tappura, K. (2009). Highly specific layers and non-label detection methods. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.
    Vikholm-Lundin, Inger ; Tappura, Kirsi. / Highly specific layers and non-label detection methods. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.
    @conference{30b849afeb854f10869c105f11735f96,
    title = "Highly specific layers and non-label detection methods",
    abstract = "We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.",
    author = "Inger Vikholm-Lundin and Kirsi Tappura",
    note = "ext. abstract; International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, ICTBSB ; Conference date: 26-01-2009 Through 27-01-2009",
    year = "2009",
    language = "English",

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    Vikholm-Lundin, I & Tappura, K 2009, 'Highly specific layers and non-label detection methods', Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland, 26/01/09 - 27/01/09.

    Highly specific layers and non-label detection methods. / Vikholm-Lundin, Inger; Tappura, Kirsi.

    2009. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.

    Research output: Contribution to conferenceConference articleScientificpeer-review

    TY - CONF

    T1 - Highly specific layers and non-label detection methods

    AU - Vikholm-Lundin, Inger

    AU - Tappura, Kirsi

    N1 - ext. abstract

    PY - 2009

    Y1 - 2009

    N2 - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

    AB - We show that the oriented highly specific layer exhibits a five-fold higher response to antigen than antibody F(ab)2-fragments that are randomly adsorbed on the sensor surface. With surface plasmon resonance CRP has been detected from serum patient samples with a detection limit that is 1000-fold of that needed for detecting cardiovascular deceases. We also report on a new concept applied to develop a synthetic receptor layer that has the same response as that of the oriented antibody layer. Very high sensitivity and selectivity of the sensing layers are even more important when non-label detection methods are used. The critical aspects of the design of a non-label molecular sensor is reported.

    M3 - Conference article

    ER -

    Vikholm-Lundin I, Tappura K. Highly specific layers and non-label detection methods. 2009. Paper presented at International Conference on Trends in Bioanalytical Sciences and Biosensors, ICTBSB 2009, Dublin, Ireland.