Hot water extraction and steam explosion as pretreatments for ethanol production from spruce bark

Katariina Kemppainen, Jenni Inkinen, Jaana Uusitalo, Tiina Nakari-Setälä, Matti Siika-aho

Research output: Contribution to journalArticleScientificpeer-review

38 Citations (Scopus)

Abstract

Spruce bark is a source of interesting polyphenolic compounds and also a potential but little studied feedstock for sugar route biorefinery processes. Enzymatic hydrolysis and fermentation of spruce bark sugars to ethanol were studied after three different pretreatments: steam explosion (SE), hot water extraction (HWE) at 80 °C, and sequential hot water extraction and steam explosion (HWE + SE), and the recovery of different components was determined during the pretreatments. The best steam explosion conditions were 5 min at 190 °C without acid catalyst based on the efficiency of enzymatic hydrolysis of the material. However, when pectinase was included in the enzyme mixture, the hydrolysis rate and yield of HWE bark was as good as that of SE and HWE + SE barks. Ethanol was produced efficiently with the yeast Saccharomyces cerevisiae from the pretreated and hydrolysed materials suggesting the suitability of spruce bark to various lignocellulosic ethanol process concepts.
Original languageEnglish
Pages (from-to)131-139
JournalBioresource Technology
Volume117
DOIs
Publication statusPublished - 2012
MoE publication typeA1 Journal article-refereed

Fingerprint

Steam
bark
Explosions
explosion
ethanol
Ethanol
Water
hydrolysis
Enzymatic hydrolysis
Sugars
Yeast
sugar
Polyphenolic compounds
Polygalacturonase
Fermentation
Feedstocks
fermentation
yeast
hot water
water extraction

Keywords

  • Bark
  • lignocellulosic ethanol
  • pretreatment
  • enzymatic hydrolysis
  • SSF

Cite this

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title = "Hot water extraction and steam explosion as pretreatments for ethanol production from spruce bark",
abstract = "Spruce bark is a source of interesting polyphenolic compounds and also a potential but little studied feedstock for sugar route biorefinery processes. Enzymatic hydrolysis and fermentation of spruce bark sugars to ethanol were studied after three different pretreatments: steam explosion (SE), hot water extraction (HWE) at 80 °C, and sequential hot water extraction and steam explosion (HWE + SE), and the recovery of different components was determined during the pretreatments. The best steam explosion conditions were 5 min at 190 °C without acid catalyst based on the efficiency of enzymatic hydrolysis of the material. However, when pectinase was included in the enzyme mixture, the hydrolysis rate and yield of HWE bark was as good as that of SE and HWE + SE barks. Ethanol was produced efficiently with the yeast Saccharomyces cerevisiae from the pretreated and hydrolysed materials suggesting the suitability of spruce bark to various lignocellulosic ethanol process concepts.",
keywords = "Bark, lignocellulosic ethanol, pretreatment, enzymatic hydrolysis, SSF",
author = "Katariina Kemppainen and Jenni Inkinen and Jaana Uusitalo and Tiina Nakari-Set{\"a}l{\"a} and Matti Siika-aho",
year = "2012",
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language = "English",
volume = "117",
pages = "131--139",
journal = "Bioresource Technology",
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Hot water extraction and steam explosion as pretreatments for ethanol production from spruce bark. / Kemppainen, Katariina; Inkinen, Jenni; Uusitalo, Jaana; Nakari-Setälä, Tiina; Siika-aho, Matti.

In: Bioresource Technology, Vol. 117, 2012, p. 131-139.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hot water extraction and steam explosion as pretreatments for ethanol production from spruce bark

AU - Kemppainen, Katariina

AU - Inkinen, Jenni

AU - Uusitalo, Jaana

AU - Nakari-Setälä, Tiina

AU - Siika-aho, Matti

PY - 2012

Y1 - 2012

N2 - Spruce bark is a source of interesting polyphenolic compounds and also a potential but little studied feedstock for sugar route biorefinery processes. Enzymatic hydrolysis and fermentation of spruce bark sugars to ethanol were studied after three different pretreatments: steam explosion (SE), hot water extraction (HWE) at 80 °C, and sequential hot water extraction and steam explosion (HWE + SE), and the recovery of different components was determined during the pretreatments. The best steam explosion conditions were 5 min at 190 °C without acid catalyst based on the efficiency of enzymatic hydrolysis of the material. However, when pectinase was included in the enzyme mixture, the hydrolysis rate and yield of HWE bark was as good as that of SE and HWE + SE barks. Ethanol was produced efficiently with the yeast Saccharomyces cerevisiae from the pretreated and hydrolysed materials suggesting the suitability of spruce bark to various lignocellulosic ethanol process concepts.

AB - Spruce bark is a source of interesting polyphenolic compounds and also a potential but little studied feedstock for sugar route biorefinery processes. Enzymatic hydrolysis and fermentation of spruce bark sugars to ethanol were studied after three different pretreatments: steam explosion (SE), hot water extraction (HWE) at 80 °C, and sequential hot water extraction and steam explosion (HWE + SE), and the recovery of different components was determined during the pretreatments. The best steam explosion conditions were 5 min at 190 °C without acid catalyst based on the efficiency of enzymatic hydrolysis of the material. However, when pectinase was included in the enzyme mixture, the hydrolysis rate and yield of HWE bark was as good as that of SE and HWE + SE barks. Ethanol was produced efficiently with the yeast Saccharomyces cerevisiae from the pretreated and hydrolysed materials suggesting the suitability of spruce bark to various lignocellulosic ethanol process concepts.

KW - Bark

KW - lignocellulosic ethanol

KW - pretreatment

KW - enzymatic hydrolysis

KW - SSF

U2 - 10.1016/j.biortech.2012.04.080

DO - 10.1016/j.biortech.2012.04.080

M3 - Article

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SP - 131

EP - 139

JO - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -