Human alfa3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNacbeta1-4(Fucalfa1-3)GlcNAc beta1-4R determinant at the nonreducing terminus

Jari Natunen, Olli Aitio, Jari Helin, Hannu Maaheimo, Ritva Niemelä, Sami Heikkinen, Ossi Renkonen

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Gal beta1-4(Fuc alpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAc beta1-4GlcNAc, generating GalNAc beta1-4(Fuc alpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAc beta1-4(Fuc alpha1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.
Original languageEnglish
Pages (from-to)209-216
Number of pages8
JournalGlycobiology
Volume11
Issue number3
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

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Fucosyltransferases
Chitin
Fucose
Oligosaccharides
Mesorhizobium
Acetylglucosamine
Human Milk
Galactose
Mass spectrometry
Mass Spectrometry
Spectrum Analysis
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance
Spectroscopy
Antigens
Enzymes
Experiments

Cite this

Natunen, Jari ; Aitio, Olli ; Helin, Jari ; Maaheimo, Hannu ; Niemelä, Ritva ; Heikkinen, Sami ; Renkonen, Ossi. / Human alfa3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNacbeta1-4(Fucalfa1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. In: Glycobiology. 2001 ; Vol. 11, No. 3. pp. 209-216.
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abstract = "Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Gal beta1-4(Fuc alpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAc beta1-4GlcNAc, generating GalNAc beta1-4(Fuc alpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAc beta1-4(Fuc alpha1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.",
author = "Jari Natunen and Olli Aitio and Jari Helin and Hannu Maaheimo and Ritva Niemel{\"a} and Sami Heikkinen and Ossi Renkonen",
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Human alfa3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNacbeta1-4(Fucalfa1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. / Natunen, Jari; Aitio, Olli; Helin, Jari; Maaheimo, Hannu; Niemelä, Ritva; Heikkinen, Sami; Renkonen, Ossi.

In: Glycobiology, Vol. 11, No. 3, 2001, p. 209-216.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Human alfa3-fucosyltransferases convert chitin oligosaccharides to products containing a GlcNacbeta1-4(Fucalfa1-3)GlcNAc beta1-4R determinant at the nonreducing terminus

AU - Natunen, Jari

AU - Aitio, Olli

AU - Helin, Jari

AU - Maaheimo, Hannu

AU - Niemelä, Ritva

AU - Heikkinen, Sami

AU - Renkonen, Ossi

PY - 2001

Y1 - 2001

N2 - Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Gal beta1-4(Fuc alpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAc beta1-4GlcNAc, generating GalNAc beta1-4(Fuc alpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAc beta1-4(Fuc alpha1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.

AB - Human alpha3-fucosyltransferases (Fuc-Ts) are known to convert N-acetyllactosamine to Gal beta1-4(Fuc alpha1-3)GlcNAc (Lewis x antigen); some of them transfer fucose also to GalNAc beta1-4GlcNAc, generating GalNAc beta1-4(Fuc alpha1-3)GlcNAc determinants. Here, we report that recombinant forms of Fuc-TV and Fuc-TVI as well as Fuc-Ts of human milk converted chitin oligosaccharides of 2-4 GlcNAc units efficiently to products containing a GlcNAc beta1-4(Fuc alpha1-3)GlcNAc beta1-4R determinant at the nonreducing terminus. The product structures were identified by mass spectrometry and nuclear magnetic resonance experiments; rotating frame nuclear Overhauser spectroscopy data suggested that the fucose and the distal N-acetylglucosamine are stacked in the same way as the fucose and the distal galactose of the Lewis x determinant. The products closely resembled a nodulation factor of Mesorhizobium loti but were distinct from nodulation signals generated by NodZ-enzyme.

M3 - Article

VL - 11

SP - 209

EP - 216

JO - Glycobiology

JF - Glycobiology

SN - 0959-6658

IS - 3

ER -