Human papillomavirus 16 E5 modulates the expression of host microRNAs

Dario Greco; Nina Kivi; Kui Qian; Suvi-Katri Leivonen; Petri Auvinen; Eeva Auvinen

doi:10.1371/journal.pone.0021646

Human papillomavirus 16 E5 modulates the expression of host microRNAs

Dario Greco, Nina Kivi, Kui Qian, Suvi-Katri Leivonen, Petri Auvinen, Eeva Auvinen (Corresponding Author)

VTT Technical Research Centre of Finland

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. HPV 16 encodes three oncogenes, E5, E6, and E7, of which E5 is the least studied so far. Its roles in regulating replication and pathogenesis of HPV are not fully understood. Here we utilize high-throughput screening to coordinately investigate the effect of E5 on the expression of host protein-coding and microRNA genes. MicroRNAs form a class of 22nt long noncoding RNAs with regulatory activity. Among the altered cellular microRNAs we focus on the alteration in the expression of miR-146a, miR-203 and miR-324-5p and their target genes in a time interval of 96 hours of E5 induction. Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E5 protein.

Original language	English
Article number	e21646
Number of pages	13
Journal	PLoS ONE
Volume	6
Issue number	7
DOIs	https://doi.org/10.1371/journal.pone.0021646
Publication status	Published - 2011
MoE publication type	A1 Journal article-refereed

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title = "Human papillomavirus 16 E5 modulates the expression of host microRNAs",

abstract = "Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. HPV 16 encodes three oncogenes, E5, E6, and E7, of which E5 is the least studied so far. Its roles in regulating replication and pathogenesis of HPV are not fully understood. Here we utilize high-throughput screening to coordinately investigate the effect of E5 on the expression of host protein-coding and microRNA genes. MicroRNAs form a class of 22nt long noncoding RNAs with regulatory activity. Among the altered cellular microRNAs we focus on the alteration in the expression of miR-146a, miR-203 and miR-324-5p and their target genes in a time interval of 96 hours of E5 induction. Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E5 protein.",

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T1 - Human papillomavirus 16 E5 modulates the expression of host microRNAs

AU - Greco, Dario

AU - Kivi, Nina

AU - Qian, Kui

AU - Leivonen, Suvi-Katri

AU - Auvinen, Petri

AU - Auvinen, Eeva

PY - 2011

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AB - Human papillomavirus (HPV) infection is a prerequisite of developing cervical cancer, approximately half of which are associated with HPV type 16. HPV 16 encodes three oncogenes, E5, E6, and E7, of which E5 is the least studied so far. Its roles in regulating replication and pathogenesis of HPV are not fully understood. Here we utilize high-throughput screening to coordinately investigate the effect of E5 on the expression of host protein-coding and microRNA genes. MicroRNAs form a class of 22nt long noncoding RNAs with regulatory activity. Among the altered cellular microRNAs we focus on the alteration in the expression of miR-146a, miR-203 and miR-324-5p and their target genes in a time interval of 96 hours of E5 induction. Our results indicate that HPV infection and subsequent transformation take place through complex regulatory patterns of gene expression in the host cells, part of which are regulated by the E5 protein.

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Human papillomavirus 16 E5 modulates the expression of host microRNAs

Abstract

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