Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance

Inger Vikholm-Lundin (Corresponding Author), Reetta Piskonen, Martin Albers

Research output: Contribution to journalArticleScientificpeer-review

23 Citations (Scopus)

Abstract

We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remaining free space was filled with polymer and (b) SH-ssDNA and the polymer was attached onto the surface from the same solution. The immobilisation methods were compared for various concentrations of SH-ssDNA. Hybridisation was dependent on both the immobilisation method and the concentration of the components. The highest hybridisation was obtained when SH-ssDNA and the polymer was immobilised from the same solution at low SH-ssDNA concentration or when high concentrations of oligos were spread onto the surface and the surface was post-treated with polymer. The target response corresponded to a surface coverage of 100 ± 15 ng/cm2. The same surface coverage on hybridisation was also obtained when low concentration of SH-ssDNA and polymer was attached onto the surface from the same solution. The non-specific binding of sample DNA was very low at optimal concentrations due to the polymer and the hybridisation was linearly dependent on target concentration.
Original languageEnglish
Pages (from-to)1323-1329
JournalBiosensors & Bioelectronics
Volume22
Issue number7
DOIs
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

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Surface Plasmon Resonance
Oligonucleotides
Surface plasmon resonance
Polymers
Immobilization
DNA
Single-Stranded DNA
Polyacrylates
Sulfhydryl Compounds
Gold
Kinetics

Keywords

  • SH-ssDNA
  • Oligonucleotides
  • Non-specific binding
  • Repellent
  • polymers
  • Surface plasmon resonance
  • Hybridisation

Cite this

Vikholm-Lundin, Inger ; Piskonen, Reetta ; Albers, Martin. / Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance. In: Biosensors & Bioelectronics. 2007 ; Vol. 22, No. 7. pp. 1323-1329.
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Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance. / Vikholm-Lundin, Inger (Corresponding Author); Piskonen, Reetta; Albers, Martin.

In: Biosensors & Bioelectronics, Vol. 22, No. 7, 2007, p. 1323-1329.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance

AU - Vikholm-Lundin, Inger

AU - Piskonen, Reetta

AU - Albers, Martin

PY - 2007

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N2 - We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remaining free space was filled with polymer and (b) SH-ssDNA and the polymer was attached onto the surface from the same solution. The immobilisation methods were compared for various concentrations of SH-ssDNA. Hybridisation was dependent on both the immobilisation method and the concentration of the components. The highest hybridisation was obtained when SH-ssDNA and the polymer was immobilised from the same solution at low SH-ssDNA concentration or when high concentrations of oligos were spread onto the surface and the surface was post-treated with polymer. The target response corresponded to a surface coverage of 100 ± 15 ng/cm2. The same surface coverage on hybridisation was also obtained when low concentration of SH-ssDNA and polymer was attached onto the surface from the same solution. The non-specific binding of sample DNA was very low at optimal concentrations due to the polymer and the hybridisation was linearly dependent on target concentration.

AB - We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remaining free space was filled with polymer and (b) SH-ssDNA and the polymer was attached onto the surface from the same solution. The immobilisation methods were compared for various concentrations of SH-ssDNA. Hybridisation was dependent on both the immobilisation method and the concentration of the components. The highest hybridisation was obtained when SH-ssDNA and the polymer was immobilised from the same solution at low SH-ssDNA concentration or when high concentrations of oligos were spread onto the surface and the surface was post-treated with polymer. The target response corresponded to a surface coverage of 100 ± 15 ng/cm2. The same surface coverage on hybridisation was also obtained when low concentration of SH-ssDNA and polymer was attached onto the surface from the same solution. The non-specific binding of sample DNA was very low at optimal concentrations due to the polymer and the hybridisation was linearly dependent on target concentration.

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KW - Repellent

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KW - Hybridisation

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