Abstract
We have investigated the hybridisation of thiol-modified single-stranded
DNA embedded in a polyacrylamide layer through the technique of surface
plasmon resonance (SPR). Kinetic studies were carried out by two different
immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the
remaining free space was filled with polymer and (b) SH-ssDNA and the polymer
was attached onto the surface from the same solution. The immobilisation
methods were compared for various concentrations of SH-ssDNA. Hybridisation
was dependent on both the immobilisation method and the concentration of the
components. The highest hybridisation was obtained when SH-ssDNA and the
polymer was immobilised from the same solution at low SH-ssDNA concentration
or when high concentrations of oligos were spread onto the surface and the
surface was post-treated with polymer. The target response corresponded to a
surface coverage of 100 ± 15 ng/cm2. The same surface coverage on
hybridisation was also obtained when low concentration of SH-ssDNA and polymer
was attached onto the surface from the same solution. The non-specific
binding of sample DNA was very low at optimal concentrations due to the
polymer and the hybridisation was linearly dependent on target concentration.
Original language | English |
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Pages (from-to) | 1323-1329 |
Journal | Biosensors & Bioelectronics |
Volume | 22 |
Issue number | 7 |
DOIs | |
Publication status | Published - 2007 |
MoE publication type | A1 Journal article-refereed |
Keywords
- SH-ssDNA
- Oligonucleotides
- Non-specific binding
- Repellent
- polymers
- Surface plasmon resonance
- Hybridisation