Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1323-1329 |
| Journal | Biosensors & Bioelectronics |
| Volume | 22 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - 2007 |
| MoE publication type | A1 Journal article-refereed |
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Keywords
- SH-ssDNA
- Oligonucleotides
- Non-specific binding
- Repellent
- polymers
- Surface plasmon resonance
- Hybridisation
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Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance. / Vikholm-Lundin, Inger (Corresponding Author); Piskonen, Reetta; Albers, Martin.
In: Biosensors & Bioelectronics, Vol. 22, No. 7, 2007, p. 1323-1329.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Hybridisation of surface-immobilised single-stranded oligonucleotides and polymer monitored by surface plasmon resonance
AU - Vikholm-Lundin, Inger
AU - Piskonen, Reetta
AU - Albers, Martin
PY - 2007
Y1 - 2007
N2 - We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remaining free space was filled with polymer and (b) SH-ssDNA and the polymer was attached onto the surface from the same solution. The immobilisation methods were compared for various concentrations of SH-ssDNA. Hybridisation was dependent on both the immobilisation method and the concentration of the components. The highest hybridisation was obtained when SH-ssDNA and the polymer was immobilised from the same solution at low SH-ssDNA concentration or when high concentrations of oligos were spread onto the surface and the surface was post-treated with polymer. The target response corresponded to a surface coverage of 100 ± 15 ng/cm2. The same surface coverage on hybridisation was also obtained when low concentration of SH-ssDNA and polymer was attached onto the surface from the same solution. The non-specific binding of sample DNA was very low at optimal concentrations due to the polymer and the hybridisation was linearly dependent on target concentration.
AB - We have investigated the hybridisation of thiol-modified single-stranded DNA embedded in a polyacrylamide layer through the technique of surface plasmon resonance (SPR). Kinetic studies were carried out by two different immobilisation methods: (a) SH-ssDNA was firstly attached on gold and the remaining free space was filled with polymer and (b) SH-ssDNA and the polymer was attached onto the surface from the same solution. The immobilisation methods were compared for various concentrations of SH-ssDNA. Hybridisation was dependent on both the immobilisation method and the concentration of the components. The highest hybridisation was obtained when SH-ssDNA and the polymer was immobilised from the same solution at low SH-ssDNA concentration or when high concentrations of oligos were spread onto the surface and the surface was post-treated with polymer. The target response corresponded to a surface coverage of 100 ± 15 ng/cm2. The same surface coverage on hybridisation was also obtained when low concentration of SH-ssDNA and polymer was attached onto the surface from the same solution. The non-specific binding of sample DNA was very low at optimal concentrations due to the polymer and the hybridisation was linearly dependent on target concentration.
KW - SH-ssDNA
KW - Oligonucleotides
KW - Non-specific binding
KW - Repellent
KW - polymers
KW - Surface plasmon resonance
KW - Hybridisation
U2 - 10.1016/j.bios.2006.05.029
DO - 10.1016/j.bios.2006.05.029
M3 - Article
VL - 22
SP - 1323
EP - 1329
JO - Biosensors & Bioelectronics
JF - Biosensors & Bioelectronics
SN - 0956-5663
IS - 7
ER -