Hydrochloric acid extractable protein patterns of Pectinatus cerevisiophilus strains

Elias Hakalehto, Auli Haikara (Corresponding Author), Tor-Magnus Enari, Kari Lounatmaa

Research output: Contribution to journalArticleScientificpeer-review

14 Citations (Scopus)

Abstract

Pectinatus cerevisiophilus strains were subjected to mild hydrochloric acid extraction. Electron microscopic examination revealed that the bacterial cells still retained their original form. The extract contained vesicles, derived mostly from the outer membranes of the bacteria. The extracted material from ten isolates was studied by SDS polyacrylamide gel electrophoresis and a number of different protein patterns were found. These allowed two main groups and four subgroups to be distinguished among the isolates. The molecular weights of the major proteins varied between 13 and 62 kilodaltons. The mild hydrochloric acid extractable protein patterns provide a useful tool for typing different strains.

Original languageEnglish
Pages (from-to)209 - 216
Number of pages8
JournalFood Microbiology
Volume1
Issue number3
DOIs
Publication statusPublished - 1984
MoE publication typeA1 Journal article-refereed

Fingerprint

Pectinatus
Hydrochloric Acid
hydrochloric acid
Proteins
proteins
polyacrylamide gel electrophoresis
Polyacrylamide Gel Electrophoresis
Molecular Weight
electrons
Electrons
molecular weight
Bacteria
Membranes
bacteria
extracts
cells

Keywords

  • Pectinatus

Cite this

Hakalehto, Elias ; Haikara, Auli ; Enari, Tor-Magnus ; Lounatmaa, Kari. / Hydrochloric acid extractable protein patterns of Pectinatus cerevisiophilus strains. In: Food Microbiology. 1984 ; Vol. 1, No. 3. pp. 209 - 216.
@article{c646374b815243dc8d00438965377fcc,
title = "Hydrochloric acid extractable protein patterns of Pectinatus cerevisiophilus strains",
abstract = "Pectinatus cerevisiophilus strains were subjected to mild hydrochloric acid extraction. Electron microscopic examination revealed that the bacterial cells still retained their original form. The extract contained vesicles, derived mostly from the outer membranes of the bacteria. The extracted material from ten isolates was studied by SDS polyacrylamide gel electrophoresis and a number of different protein patterns were found. These allowed two main groups and four subgroups to be distinguished among the isolates. The molecular weights of the major proteins varied between 13 and 62 kilodaltons. The mild hydrochloric acid extractable protein patterns provide a useful tool for typing different strains.",
keywords = "Pectinatus",
author = "Elias Hakalehto and Auli Haikara and Tor-Magnus Enari and Kari Lounatmaa",
year = "1984",
doi = "10.1016/0740-0020(84)90036-4",
language = "English",
volume = "1",
pages = "209 -- 216",
journal = "Food Microbiology",
issn = "0740-0020",
publisher = "Elsevier",
number = "3",

}

Hydrochloric acid extractable protein patterns of Pectinatus cerevisiophilus strains. / Hakalehto, Elias; Haikara, Auli (Corresponding Author); Enari, Tor-Magnus; Lounatmaa, Kari.

In: Food Microbiology, Vol. 1, No. 3, 1984, p. 209 - 216.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Hydrochloric acid extractable protein patterns of Pectinatus cerevisiophilus strains

AU - Hakalehto, Elias

AU - Haikara, Auli

AU - Enari, Tor-Magnus

AU - Lounatmaa, Kari

PY - 1984

Y1 - 1984

N2 - Pectinatus cerevisiophilus strains were subjected to mild hydrochloric acid extraction. Electron microscopic examination revealed that the bacterial cells still retained their original form. The extract contained vesicles, derived mostly from the outer membranes of the bacteria. The extracted material from ten isolates was studied by SDS polyacrylamide gel electrophoresis and a number of different protein patterns were found. These allowed two main groups and four subgroups to be distinguished among the isolates. The molecular weights of the major proteins varied between 13 and 62 kilodaltons. The mild hydrochloric acid extractable protein patterns provide a useful tool for typing different strains.

AB - Pectinatus cerevisiophilus strains were subjected to mild hydrochloric acid extraction. Electron microscopic examination revealed that the bacterial cells still retained their original form. The extract contained vesicles, derived mostly from the outer membranes of the bacteria. The extracted material from ten isolates was studied by SDS polyacrylamide gel electrophoresis and a number of different protein patterns were found. These allowed two main groups and four subgroups to be distinguished among the isolates. The molecular weights of the major proteins varied between 13 and 62 kilodaltons. The mild hydrochloric acid extractable protein patterns provide a useful tool for typing different strains.

KW - Pectinatus

U2 - 10.1016/0740-0020(84)90036-4

DO - 10.1016/0740-0020(84)90036-4

M3 - Article

VL - 1

SP - 209

EP - 216

JO - Food Microbiology

JF - Food Microbiology

SN - 0740-0020

IS - 3

ER -